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Preparation and method of immunoregulatory peptide

An immune regulation and immune polypeptide technology, applied in the biological field, can solve the problem of no relevant reports on the preparation of polypeptides, and achieve the effects of simple and efficient enzymatic hydrolysis technology and avoiding cost waste.

Active Publication Date: 2020-01-07
FUZHOU UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been many studies on the chemical composition and medicinal efficacy of Pseudostellariae Pseudostellariae, but there is no relevant report on the preparation of polypeptides by proteolysis from Pseudostellariae Pseudostellariae

Method used

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  • Preparation and method of immunoregulatory peptide
  • Preparation and method of immunoregulatory peptide
  • Preparation and method of immunoregulatory peptide

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Experimental program
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Embodiment 1

[0023] The amino acid sequence of the immunomodulatory peptide of the present invention is Arg-Gly-Pro-Pro-Pro.

[0024] The preparation method is as follows:

[0025] Step 1: Grind Pseudostellariae Radix Pseudostellariae into powder with a traditional Chinese medicine grinder, pass through a 60-mesh sieve; weigh 5.0 g Pseudostellariae Pseudostellariae powder, add 105 mL of 0.27 mol / L sodium hydroxide solution, and extract at 52.1 °C for 1 h, 10,000 After centrifuging at rpm for 20 min, take the supernatant, adjust the pH to 3.0 with 1 mol / L hydrochloric acid, let it stand for 1 h, centrifuge at 5,000 rpm for 20 min, take the precipitate, and freeze-dry it to obtain Pseudostellaria protein.

[0026] Step 2: Weigh 10.0 g of Pseudostellariae protein, add 100 mL of deionized water, adjust the pH to 2.0, add 0.8 g of pepsin, place it at 37 °C for 5.0 h, adjust the pH to 7.0, add 0.8 g of pancreatic Protease, hydrolyzed at 37 ℃ for 3.0 h, boiled in a boiling water bath for 10 min ...

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Abstract

The invention provides a method for separating and purifying immunoregulatory peptide by using radix pseudostellariae. The method for separating and purifying the immunoregulatory peptide by using theradix pseudostellariae comprises the steps that the radix pseudostellariae is used as a raw material, through protein extraction and dual-step enzymolysis of pepsase and trypsin, separation and purification and freeze drying are carried out to obtain specific immunity polypeptide, the molecular weight of the specific immunity polypeptide is 522 Da, and an amino acid total sequence of the specificimmunity polypeptide is Arg-Gly-Pro-Pro-Pro. According to the method for separating and purifying the immunoregulatory peptide by using the radix pseudostellariae, the defects of immunoregulatory drugs are overcome, the anxiety of the public to artificial immunity regulators is eliminated, and a theoretical basis is laid for developing immune polypeptide based on a food source and searching the wide application of the immune polypeptide in food and medicine.

Description

technical field [0001] The invention relates to a method for separating and purifying immunoregulatory peptides from Radix Pseudostellariae, belonging to the field of biotechnology. Background technique [0002] Immunity is a specific physiological reaction that occurs when the body contacts "antigenic foreign bodies" or "foreign molecules", and its main purpose is to maintain the homeostasis of the body. The immune system, which is responsible for carrying out the immune function of the body, is a network of cells, tissues and organs that eliminates potentially harmful substances such as bacteria, viruses, fungi, protozoa, and prevents the growth of cancer cells. In the body's survival, the immune system acts as the first line of defense against pathogens, providing protection before bodily functions are impaired. However, the immune system is also affected by many factors, including stress, unhealthy lifestyle, pathogens and antigens, which can damage the body's immune sy...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K1/20C12P21/06
CPCC07K7/06C12P21/06
Inventor 汪少芸杨倩蔡茜茜陈旭田永奇
Owner FUZHOU UNIVERSITY
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