Preparation, separation and purification method for immunological peptide
An immune regulation, separation and purification technology, applied in the biological field, can solve the problems of no relevant reports on the preparation of polypeptides, achieve the effect of simple and efficient enzymatic hydrolysis technology, and avoid cost waste
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[0022] Example 1
[0023] The amino acid sequence of the immunomodulatory peptide of the present invention is Tyr-Gly-Pro-Ser-Ser-Tyr-Gly-Tyr-Gly.
[0024] The preparation method is as follows:
[0025] The first step: use Chinese medicine pulverizer to pulverize Radix sylvestris into powder, pass through a 60-mesh sieve; Weigh 5.0 g Radix sylvestris powder, add 100 mL of 0.3 mol / L sodium hydroxide solution, extract for 1.5 h at 52.1 ℃, 10,000 After centrifugation at rpm for 20 min, the supernatant was taken, and the pH was adjusted to 2.5 with 2 mol / L hydrochloric acid, and the mixture was allowed to stand for 1 h. Centrifuge at 5,000 rpm for 20 min. The precipitate was taken and freeze-dried to obtain the protein.
[0026] The second step: Weigh 10.0 g of Radix Ginseng protein, add 100 mL of deionized water, adjust the pH to 2.0, add 0.8 g of pepsin, and place at 37 ℃ for enzymatic hydrolysis for 5.0 h, adjust the pH to 7.0, add 0.8 g of pancreas Put the protease in 37 ℃ for 3.0 h,...
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