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Preparation, separation and purification method for immunological peptide

An immune regulation, separation and purification technology, applied in the biological field, can solve the problems of no relevant reports on the preparation of polypeptides, achieve the effect of simple and efficient enzymatic hydrolysis technology, and avoid cost waste

Active Publication Date: 2019-12-20
FUZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been many studies on the chemical composition and medicinal efficacy of Pseudostellariae Pseudostellariae, but there is no relevant report on the preparation of polypeptides by proteolysis from Pseudostellariae Pseudostellariae

Method used

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  • Preparation, separation and purification method for immunological peptide
  • Preparation, separation and purification method for immunological peptide
  • Preparation, separation and purification method for immunological peptide

Examples

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Embodiment 1

[0023] The amino acid sequence of the immunomodulatory peptide of the present invention is Tyr-Gly-Pro-Ser-Ser-Tyr-Gly-Tyr-Gly.

[0024] The preparation method is as follows:

[0025] Step 1: Grind Pseudostellariae Radix Pseudostellariae into powder with a traditional Chinese medicine grinder, pass through a 60-mesh sieve; weigh 5.0 g Pseudostellariae Pseudostellariae powder, add 100 mL of 0.3 mol / L sodium hydroxide solution, extract at 52.1 °C for 1.5 h, 10,000 After centrifuging at rpm for 20 min, take the supernatant, adjust the pH to 2.5 with 2 mol / L hydrochloric acid, let it stand for 1 h, centrifuge at 5,000 rpm for 20 min, take the precipitate, and freeze-dry it to obtain Pseudostellaria protein.

[0026] Step 2: Weigh 10.0 g of Pseudostellariae protein, add 100 mL of deionized water, adjust the pH to 2.0, add 0.8 g of pepsin, place it at 37 °C for 5.0 h, adjust the pH to 7.0, add 0.8 g of pancreatic Protease, placed in 37 ℃ for 3.0 h, boiled in a boiling water bath fo...

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Abstract

The invention provides a method for carrying out separation and purification on pseudostellariae radix to obtain an immunoregulation peptide. The method takes the pseudostellariae radix as a raw material, purification and separation are carried out through protein extraction and the double-step enzymolysis of pepsin and trypsin to obtain a specific immune polypeptide, the molecular weight of the specific immune polypeptide is 949 Da, and the amino acid complete sequence of the specific immune polypeptide is Tyr-Gly-Pro-Ser-Ser-Tyr-Gly-Tyr-Gly. The method overcomes the defects of an existing immunoregulation medicine, in addition, the worry of the public for an artificial immunomodulator is eliminated, and a certain theoretical foundation is laid for developing the immune polypeptide basedon a food source and exploring the wide application of the immune polypeptide in food and medicine fields.

Description

technical field [0001] The invention relates to a method for separating and purifying immunoregulatory peptides from Radix Pseudostellariae, belonging to the field of biotechnology. Background technique [0002] Immunity is a specific physiological reaction that occurs when the body contacts "antigenic foreign bodies" or "foreign molecules", and its main purpose is to maintain the homeostasis of the body. The immune system is a network of cells, tissues, and organs that works to eliminate potentially harmful substances such as bacteria, viruses, fungi, protozoa, and to stop the growth of cancer cells. In the body's survival, the immune system acts as the first line of defense against pathogens, providing protection before bodily functions are impaired. However, the immune system is also affected by many factors, including stress, unhealthy lifestyle, pathogens and antigens, which can damage the body's immune system. As a result, drugs used to regulate the human immune resp...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12P21/06C07K1/20C07K1/16C07K1/14
CPCC07K7/06C12P21/06
Inventor 汪少芸杨倩蔡茜茜陈旭田永奇
Owner FUZHOU UNIVERSITY
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