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Cucumber CsLsi2 gene, protein coded by same and application of cucumber CsLsi2 gene

A gene encoding and cucumber technology, applied in the field of genetic engineering, can solve problems such as difficulty in improving quality and difficulty in cloning cucumber genes

Active Publication Date: 2020-01-10
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the technical problems of cucumber gene cloning and quality improvement in the prior art, the present invention provides a cucumber CsLsi2 gene and its encoded protein and its application

Method used

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  • Cucumber CsLsi2 gene, protein coded by same and application of cucumber CsLsi2 gene
  • Cucumber CsLsi2 gene, protein coded by same and application of cucumber CsLsi2 gene
  • Cucumber CsLsi2 gene, protein coded by same and application of cucumber CsLsi2 gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] This embodiment provides a method for observing the fruit frost morphology on the surface of cucumber fruit by electron microscope scanning

[0040] 1.1. Materials: Cucumber 3402, Black Seed Pumpkin and Beinong Bright Anvil

[0041] (1) Fresh fruit samples of cucumber 3402 grafted on itself, grafted on black-seeded pumpkin, and grafted on Beinongliang anvil were fixed in 3% (v / v) glutaraldehyde for 24 hours;

[0042] (2) Take out the fruit sample, then wash it three times in pH=6.8, 0.1M PBS (phosphate buffer sodium), 10 min each time;

[0043] (3) Fixing with osmium tetroxide with a volume ratio of 1%;

[0044] (4) The samples were dehydrated and dried in alcohols with a concentration of 50%, 70%, 80%, 90%, and 100% in sequence, each time for 10 minutes;

[0045] (5) The sample is washed twice in 100% acetone, each time for 15 minutes;

[0046] (6) The sample was washed 3 times with 100% isoamyl acetate, each time for 15 minutes;

[0047] (7) In liquid CO 2 (Bal-Tec...

Embodiment 2

[0051] This embodiment provides a method for increasing, suppressing and measuring silicon content of silicon in the field, the specific steps are as follows:

[0052] 1. Materials

[0053] Cucumber 3549-1 was used as the test material. The cucumber seeds used in the experiment were sterilized, placed in an incubator at 28°C for germination, and then seedlings were raised in 72 plug trays. The seedlings were cultivated in the Jinliuhuan Agricultural Park, and the cultivation was carried out according to the standardized model. When the first true leaf is fully expanded, transplant it to protected land for field management.

[0054] 2. Test treatment

[0055] When entering the early melon stage, cucumber 3549-1 was treated with silicon increase (three concentrations) and silicon absorption inhibition (one concentration) respectively, with 3 plants for each treatment and 3 repetitions. The substance used for silicon addition is K 2 SiO 4 , with low concentration (0.02mM), ...

Embodiment 3

[0060] In this embodiment, cucumber 3548-1 and cucumber 3549-1 are used as test materials, and energy spectrum analysis is carried out on the fruit frost on the fruit surface by electron microscope scanning. After the surface is plated with platinum, put it into the sample chamber of a scanning electron microscope, use an accelerating voltage of 15kV to zoom in and observe the test site, and use an X-ray energy spectrum analyzer to conduct qualitative and semi-quantitative analysis of the sample.

[0061] The result is as Figure 4-Figure 6 as shown, Figure 4 It is the fruit frost comparison chart of cucumber 3548-1 and cucumber 3549-1, such as Figure 4 As shown, there was no significant difference in the total silicon content of the two fruits;

[0062] Figure 5 It is a comparison chart of the silicon content in the peel and pulp of cucumber 3548-1 and cucumber 3549-1, as shown in Figure 5 As shown, the silicon content in the pulp of cucumber 3548-1 with more fruit fr...

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Abstract

The invention relates to a cucumber CsLsi2 gene, a protein coded by the same and application of the cucumber CsLsi2 gene. The nucleotide sequence of the cucumber CsLsi2 gene is shown as SEQ ID NO: 1,and the sequence of the protein coded by the gene is shown as SEQ ID NO: 2. The cucumber CsLsi2 gene closely related to the cucumber bloom character is obtained by cloning after genome-wide association analysis and screening, the expression level of the gene is positively related to the cucumber bloom content, and early termination of amino acid translation is caused after the 754th basic group ofthe sequence, shown as SEQ ID NO: 1, of the cucumber gene is changed from C to T. Cloning and expression analysis of the cucumber CsLsi2 gene play a promoting role to a great extent in solving the problem that cucumber bloom gene resources are difficult to screen.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to the cucumber CsLsi2 gene and its encoded protein and application. Background technique [0002] Cucumbers are an important source of dietary vitamin C. Because of its high yield and good benefits, it is an existing main vegetable crop, and the quality of cucumber directly determines the benefits of producers. Appearance quality belongs to the category of quality, and fruit cream is one of the important appearance qualities of cucumbers, which affects the sales of cucumbers to a large extent. Generally speaking, varieties with less fruit frost, bright and straight melon strips are more popular in the market. For a long time, there have been disputes about the composition and formation of cucumber fruit frost. One view is that the formation of cucumber fruit frost is the result of the vertical skin surface division of the single cell of the peel, and the hairy cells f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/11C12N15/82C07K14/415A01H5/00A01H6/34
CPCC07K14/415C12N15/8261
Inventor 任华中刘兴旺张亚琦董明明翟许玲尹帅陈淑英徐硕冯钟萱
Owner CHINA AGRI UNIV
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