Primers and probe for digital PCR detection of expression quantity of metallothionein (MT)1 in argopecten irradians

Abstract

The invention discloses primers and a probe for digital PCR detection of the expression quantity of metallothionein (MT)1 in argopecten irradians. The primers and the probe are characterized in that the nucleotide sequences of the primers and the probe are as follows: the nucleotide sequence of the forward primer is 5'-gctccgatgattgccagtct-3'; the nucleotide sequence of the reverse primer is 5'-agtgctgcggaacc-3', and the nucleotide sequence of the probe is 5'-agtgctgcggaacc-3', wherein a fluorophore added into the probe is 6-carboxylfluorescein. The primers and the probe are high in accuracy and specificity, so that the time sequence change of the expression quantity of the MT1 in the argopecten irradians can be detected by utilizing a digital PCR technology, the time sequence change is taken as an index for evaluating the health condition of the cultured argopecten irradians, and an established evaluation system has potential application value in disease early-warning and forecastingof the cultured argopecten irradians. A control group does not need to be set, an internal control gene is not needed either, and the application limitation of a traditional fluorescence real-time quantitative PCR detection method is broken through.

Description

technical field [0001] The invention belongs to the technical fields of molecular biology and biochemistry, and in particular relates to a primer and a probe for detecting the expression level of bay scallop MT1 by digital PCR. Background technique [0002] MTs (metallothionein) are a class of low-molecular-weight, cysteine-rich proteins that can bind heavy metals and widely exist in organisms. One of the main physiological functions of MTs is to homeostasis regulate intracellular metal levels, that is, to participate in the regulation of essential metal elements in organisms and the detoxification of non-essential metal elements. In addition, MTs participate in the scavenging of reactive oxygen species as non-enzymatic antioxidants, which scavenge hydroxyl radicals (HO•) and superoxide anion radicals (O 2- ) is about 50-100 times that of reduced glutathione. Corresponding to the above functions, one of the important characteristics of MTs is that their expression is signi...

AI Technical Summary

Problems solved by technology

However, so far, there is no relevant report on the primers and probes for detecting the expression level of bay scallop MT1 by digital PCR, so that there is no digital PCR-based detection of bay scallop MT1 expression and use it as an indicator to evaluate the health status of cultured bay scallops

Images