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A kind of color comparison strip, color comparison card and detection method for rapid detection of histamine

A detection method and colorimetric strip technology, applied in the field of chemical detection, can solve the problems of long detection time, high cost, complicated processing, etc., and achieve the effects of easy storage and carrying, accurate and sensitive identification, and good stability.

Active Publication Date: 2022-02-22
INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the methods for detecting histamine include the national standard method (the minimum detection concentration is 50 mg / kg), immunochemical method, spectrophotometric method, etc. Most of the methods for quantitative histamine need to use some equipment for detection, which has complicated sample pretreatment. , the detection time is long and cannot meet the disadvantages of on-site detection; the histamine detection test strip based on immune response relies on highly specific antibodies, the test strip needs to be coated and modified in multiple steps, and needs to be stored in specific environmental conditions, which is costly and difficult to use. inconvenient

Method used

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  • A kind of color comparison strip, color comparison card and detection method for rapid detection of histamine
  • A kind of color comparison strip, color comparison card and detection method for rapid detection of histamine
  • A kind of color comparison strip, color comparison card and detection method for rapid detection of histamine

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Effect test

Embodiment 1

[0049] This example is used to illustrate the preparation of the colorimetric strip of the present invention.

[0050] (1) Preparation of PDA vesicles: Accurately weigh 26.2 mg of 10,12-pentacosadiynoic acid and 20.3 mg of dimyristoylphosphatidylcholine in a 10 mL chloroform flask, and stir for 2 hours in the dark; Remove the solvent on a rotary evaporator or a nitrogen blower, and a light pink film is formed on the bottom of the bottle. Measure 20mL of ultrapure water into the flask, mix well, and use a 20% power ultrasonic disperser to ultrasonically disperse for 20 minutes; PDA is formed after the dispersion is complete. The vesicle dispersion is mixed evenly, and stored at 4° C. in the dark for at least 6 hours; then polymerized by ultraviolet irradiation to obtain a PDA vesicle solution.

[0051] (2) Assembling the detection colorimetric strip: Prepare the glass cellulose membrane and the base card, cut the base card into strips with a length of 5 cm and a width of 0.4 cm...

Embodiment 2

[0057] This embodiment is used to illustrate the preparation of the standard color chart of the present invention.

[0058] Make a standard color comparison card: Prepare different concentrations of histamine standard solutions (0, 468.75μM, 937.5μM, 1.875mM, 3.75mM, 7.5mM, 15mM, 30mM, 60mM), and add 10μL dropwise to the assembled color comparison strip First, incubate at 25°C to record the color, and print a standard color card according to the depth of the reaction color and the change of the color scale.

Embodiment 3

[0060] This embodiment is used to illustrate the drawing of the standard curve for the detection of histamine by the colorimetric strip.

[0061] Use image J software to analyze the color after histamine response. The specific steps are as follows: separate the RGB image into grayscale images, invert the image for calibration, adjust the threshold to red, and select the area and data for analysis and measurement as the selected Threshold value and average gray scale, measure the same color rendering area of ​​different color comparison strips sequentially to obtain the average gray value of each color comparison strip, take different histamine concentrations as the abscissa, and the average gray value as the ordinate, draw a standard curve; like image 3 shown.

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Abstract

The invention belongs to the field of chemical detection, and relates to a colorimetric strip, a colorimetric card and a detection method for rapid detection of histamine. The colorimetric strip is prepared by a method comprising the following steps: (1) preparing PDA vesicles: mixing and stirring 10,12-pentacosadiynoic acid, dimyristoylphosphatidylcholine and an organic solvent, and then Evaporate the organic solvent, add ultra-pure water for dissolution, and carry out ultrasonic dispersion, then store the dispersion at low temperature and avoid light; then polymerize through ultraviolet irradiation to obtain the PDA vesicle dispersion; (2) Assemble the detection colorimetric strip: the adsorption The carrier is fixed on the bottom plate, and the PDA vesicle dispersion containing 0.2%-0.8% trehalose is added dropwise on the adsorption carrier as a color indicator solution to obtain the colorimetric strip. Compared with other detection methods, the colorimetric strip of the present invention does not require complicated biochemical modification and instrument detection, the colorimetric strip is easy to store and carry, and the detection result is visible to the naked eye, simple and intuitive, and low in cost.

Description

technical field [0001] The invention belongs to the field of chemical detection, and more specifically relates to a color comparison strip for rapid detection of histamine, a color comparison card for rapid detection of histamine and a rapid detection method for histamine in meat products. Background technique [0002] Histamine exists in many animals and plants and is a kind of biogenic amine. In the process of food fermentation or food spoilage, microorganisms will generate histamine under the catalysis of histidine decarboxylase, and the content of histamine in food will increase significantly at this time. Low-dose histamine participates in various physiological reactions of the human body, and has the effect of lowering blood pressure and promoting tumor treatment. However, when its content exceeds the tolerance level of the human body, it will cause histamine poisoning and allergic reactions, such as nausea, diarrhea, rash, urticaria, conjunctival hyperemia, dizziness...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/01
CPCG01N21/78G01N21/01G01N2021/0112
Inventor 高志贤韩殿鹏彭媛李双白家磊吴瑾宁保安李巧凤
Owner INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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