Lactobacillus plantarum s58 and its application in the preparation of products for alleviating obesity
A technology of Lactobacillus plantarum and obesity, which is applied in the direction of application, microbe-based methods, biochemical equipment and methods, etc., can solve the problems of nutritional metabolism disorder and reduce the quality of life of patients, and achieve the effect of expanding the scope of application and improving the value of use
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[0036] In order to make the object, technical solution and advantages of the present invention clearer, preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
[0037] 1. Isolation and identification of Lactobacillus plantarum S58
[0038] 1 Experimental materials
[0039] Naturally fermented kimchi from farmers in Chongqing.
[0040] 2 Experimental methods
[0041] 2.1 Isolation and purification of lactic acid bacteria
[0042] Carry out 10-fold gradient dilution with the pickled vegetable water taken back, successively dilute to 10 -7 . Choose 4 suitable dilutions (dilution is 10 -4 、10 -5 、10 -6 、10 -7 100 μL respectively, were spread on the MRS solid plate, and after culturing at 37°C for 48 hours, single colonies with different shapes were selected to isolate the strains by streaking on the plate. The above steps were repeated until a purified strain was obtained, and the morphology was observ...
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