Application of SF3B as target in preparation of drugs for preventing or treating breast cancer

A technology of SF3B1 and breast cancer, applied in the field of biomedicine, can solve problems such as unclear SF3B1 breast cancer

Pending Publication Date: 2020-01-31
SHANXI MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It remains unclear whether SF3B1 may be a molecular target in breast cancer

Method used

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  • Application of SF3B as target in preparation of drugs for preventing or treating breast cancer
  • Application of SF3B as target in preparation of drugs for preventing or treating breast cancer
  • Application of SF3B as target in preparation of drugs for preventing or treating breast cancer

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Experimental program
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Embodiment Construction

[0025] According to the guidelines of the local ethics committee, cancer tissue samples from 110 patients diagnosed with breast cancer were obtained from the First Affiliated Hospital of Shanxi Medical University, 40 of which had matching non-tumor control tissues. Molecular subtypes include Luminal A (38), Luminal B (38), HER2-enriched (14) and triple-negative / basal-like breast cancer (20). None of the patients received radiation or chemotherapy before surgery.

[0026] Immunohistochemistry: Paraffin-embedded formalin-fixed tissue samples were used for immunohistochemistry. Cut the specimen into 4 μm thick slices. Sections were deparaffinized and rehydrated using graded xylene and ethanol. Then the sample was washed with 3% H 2 o 2 Incubate for 10-15 min and rinse three times with distilled water. After antigen extraction in sodium citrate buffer (pH 6.0) for 2 min and blocking in goat serum, sections were incubated with specific anti-SF3B1 antibody (Abcam) diluted 1:200...

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Abstract

The invention belongs to the technical field of biological medicine, and provides a novel molecular target SF3B1 for preventing or treating breast cancer and application of the target in the parathionof drugs for preventing or treating breast cancer. The SF3B1 gene is used as a target, and the expression of the SF3B1 gene is reduced by adopting gene knockout, gene knockdown or chemical drugs. Theexpression of SF3B1 in breast cancer cells was significantly improved compared with that in normal tissue cells. SF3B1 expression level is correlated with lymph node metastasis. SF3B1 knockout in breast cancer can induce apoptosis and cell cycle arrest. SF3B1 knockout can reduce cell proliferation, colony formation, invasion and migration ability. Exon skipping is the most common splicing event after SF3B1 knockout. Researches show that SF3B1 may be an important molecular target for the treatment of breast cancer.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the application of SF3B1 as a target in the preparation of drugs for preventing or treating breast cancer. Background technique [0002] Aberrant splicing is one of the hallmarks of cancer-associated phenotypes, and tumorigenesis is often accompanied by aberrant RNA splicing. It is well known that alternative splicing (AS) is an important feature of eukaryotes, controlled by the spliceosome composed of small nuclear RNA (snRNA) and various proteins. Mutations and changes in expression levels of splicing factor genes can lead to exon loss, intron retention, misidentification of splice sites, and changes in open reading frames, thereby affecting most of the cells involved in cell cycle control, angiogenesis, apoptosis, Post-transcriptional processing and expression of metastatic genes. Cancers have many alternative splicing events that are absent in normal samples....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158
Inventor 张玲刘峰刘静程彩霞毕炀辉张燕燕宋彬张海涛卞琳高迎真
Owner SHANXI MEDICAL UNIV
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