Kit for detecting human heparin binding protein and preparation method thereof

A heparin-binding protein and kit technology, applied in the field of detection of human heparin-binding protein, can solve the problems of not covering high-concentration HBP, low sensitivity, and only reaching 0.00-500.00ng/mL
CN110806487APending Publication Date: 2020-02-18SHENZHEN AMTECH BIOENGINEERING LTD INC
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHENZHEN AMTECH BIOENGINEERING LTD INC
Publication Date
2020-02-18

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Abstract

The application discloses a kit for detecting human heparin binding protein and a preparation method thereof. The kit for latex enhanced immunoturbidimetry detection comprises a reagent 1, a reagent 2and a calibration product. The reagent 2 contains HBP-monoclonal-antibody-labeled large-sphere latex microspheres and HBP-monoclonal-antibody-labeled small-sphere latex microspheres, wherein a particle size difference between the large-sphere latex microsphere and the small-sphere latex microsphere is at least 200 nm and a surface charge difference between the small-sphere latex microspheres andthe large-sphere latex microspheres is at least 100 [mu] eq / g. According to the application, the large-sphere and small-sphere latex microspheres with the particle size difference value of at least 200nm and the surface charge difference value of at least 100 [mu]eq / g are used for HBP monoclonal antibody labeling to realize latex enhanced immunoturbidimetry detection, so that the detection sensitivity can be improved and the wider detection linear range can be obtained. A high-sensitivity and full-scale detection kit is provided for HBP clinical detection.
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Description

technical field

[0001] The present application relates to the field of detection of human heparin-binding protein, in particular to a kit for detection of human heparin-binding protein and a preparation method thereof. Background technique

[0002] Human heparin-binding protein (HBP) is a granule protein derived from neutrophils, which is mainly released by polymorphonuclear neutrophils (PMN) by external stimuli. The content of HBP in normal human blood is very low, generally not exceeding 10ng / mL. When an infection occurs, some bacteria invade the blood vessels, and substances such as the bacteria themselves or the toxins released by the bacteria stimulate the neutrophils to release HBP, resulting in an increase in the HBP content in the blood. HBP can reach 20-30ng / mL in general infection. Severe infections in the ICU will be higher, can exceed 100ng / mL, and even reach 1000ng / mL.

[0003] HBP can be used as a diagnostic indicator of local infection, an indicator of auxi...

Claims

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