Extraction and purification method of grape aromatic glycoside and library building, identification and quantification method thereof

A purification method and technology of aroma sugar, which is applied in the field of chemical analysis of natural aroma substances, can solve the problems of qualitative identification, restriction of terpene aroma glycoside identification and analysis, and limitation of terpene glycoside types, so as to ensure accuracy and reliability sexual effect

Pending Publication Date: 2020-02-21
NORTHWEST A & F UNIV
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Problems solved by technology

Although there have been many reports on the application of LC-MS technology to the analysis of terpene-like aroma glycosides, the qualitative identification of related research still stays in the preparation of glycosides, the comparison of ion fragments by mass spectrometry, a

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  • Extraction and purification method of grape aromatic glycoside and library building, identification and quantification method thereof
  • Extraction and purification method of grape aromatic glycoside and library building, identification and quantification method thereof
  • Extraction and purification method of grape aromatic glycoside and library building, identification and quantification method thereof

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Embodiment Construction

[0028] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0029] Based on the optimization work of extraction, separation and purification of grape terpene aroma glycosides, the present invention adopts ultra-high performance liquid chromatography-tandem time-of-flight mass spectrometry (UPLC-QTOF-MS) as an analytical instrument for molecular identification of terpene glycosides, and develops A mass spectral library of dozens of grape terpene-like aroma glycosides containing typical ion fragment information was obtained, and a convenient and applicable LC-MS qualitative and quantitative analysis method for terpene-like aroma glycosides was given.

[0030] The premise of the present inven...

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Abstract

The invention discloses an extraction and purification method of a grape aromatic glycoside and a library building, identification and quantification method thereof. The extraction and purification method comprises the steps of selecting and peeling grape fruits, carrying out extraction, separation and purification of a terpene aromatic glycoside, building a terpene aromatic glycoside mass spectral library, performing LC-MS analysis of the terpene aromatic glycoside, using the terpene aromatic glycoside mass spectral library, and performing qualitative and quantitative analysis of the terpenearomatic glycoside. The extraction and purification method is low in cost and high in extraction efficiency, and the precision and reliability of research are guaranteed through system matching establishment of the method. The methods established by the research are simple and feasible in actual analysis, and have good popularization and application values.

Description

[0001] 1. Technical field: [0002] The invention relates to a chemical analysis method for natural aroma substances, in particular to a method for extracting and purifying grape aroma glycosides and a method for establishing a database, identifying and quantifying them. [0003] 2. Background technology: [0004] Terpene-like aroma glycosides are important aroma precursors in fruits, tea, flowers and their processed products. They have the potential to produce free terpene-like aroma substances by hydrolysis, so they have become a research hotspot at this stage. The typical aroma characteristics of wine depend on the release and hydrolysis of aroma precursors on grape skins during fermentation. Terpene aroma glycosides are one of the most important types. in accordance with. Due to the difficulty in the artificial synthesis of terpene-like aroma glycosides and the lack of standard products of terpene-like aroma glycosides on the market, the chemical structure of grape terpene...

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Application Information

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IPC IPC(8): G01N30/06G01N30/12G01N30/14G01N30/86G01N24/08C40B50/06
CPCC40B50/06G01N24/087G01N30/06G01N30/12G01N30/14G01N30/86G01N30/8679G01N2030/126G01N2030/146Y02A90/30
Inventor 陶永胜宋鸿聪张军翔
Owner NORTHWEST A & F UNIV
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