Digital PCR device and single-pump droplet generation system thereof

Abstract

The invention discloses a single-pump droplet generation system, the single-pump droplet generation system comprises a single pump, a water phase injector connected with the output end of the single pump and used for injecting a water phase, a flow guide pipe communicated with an injection head of the water phase injector, an adjusting mechanism used for clamping the flow guide pipe and adjustingthe height of the tail end of the flow guide pipe, a liquid collecting bottle and a centrifugal pipe arranged in the liquid collecting bottle and used for containing an oil phase. And the tail end ofthe flow guide pipe extends into the centrifugal pipe, and a preset distance for generating liquid drops with preset sizes is kept between the tail end of the flow guide pipe and the bottom end of thecentrifugal pipe. Thus, the single pump is used as a power source to drive the water phase injector to inject the water phase into the flow guide pipe, and then the oil phase is contained in the centrifugal pipe, so that the injected water phase and the oil phase are mixed to generate droplets required by a reaction, and on the basis of smoothly generating digital PCR droplets, the space occupation of equipment and the system structure complexity can be reduced; meanwhile, the research cost is reduced. The invention also discloses a digital PCR device, which has the beneficial effects as described above.

Description

technical field [0001] The invention relates to the technical field of PCR, in particular to a single-pump droplet generation system. The invention also relates to a digital PCR device. Background technique [0002] At present, digital polymerase chain reaction (PCR, Polymerase Chain Reaction) devices based on microfluidic technology are more and more widely used in the field of absolute quantification of nucleic acid molecules, so digital PCR devices also need to meet the needs of various working environments. [0003] Existing digital PCR devices need to be divided into three independent parts, namely droplet generation, amplification reaction, fluorescence detection and digital statistics. The three independent parts are relatively large in size and cannot meet the needs of various environments. All commercial digital PCR devices and most laboratory-made droplet generation devices require two pumps, one pump controls the oil phase and the other pump controls the water ph...

AI Technical Summary

Problems solved by technology

This device that independently controls the water phase and the oil phase through two pumps requires a complex control mechanism, and the equipment is highly specialized, but it greatly increases the volume of the entire system, and the cost is too high, which is not conducive to carrying out basic research work on PCR.

At the same time, it is difficult to accurately control important parameters such as the size of the droplets by controlling the water phase and the oil phase through two pumps to generate mixed droplets. It is necessary to coordinate and control various operating parameters of the two pumps at the same time, and there are too many influencing factors.

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