Method for detecting quality of double-winter capsules

Abstract

The invention relates to a method for detecting the quality of double-winter capsules. The method comprises the following steps that: 1, effective components of the five medicinal materials of cape jasmine, astragalus membranaceus, oldenlandia diffusa, radix ophiopogonis and picrasma quassioides in a composition are qualitatively identified; and the effective components in the cape jasmine and astragalus membranaceus are qualitatively detected. The detection method has the effects of the strong specificity, the good durability, the good recovery rate and the like through methodological investigation, can effectively detect the product quality condition and further improves the product quality.

Description

technical field [0001] The invention relates to the field of drug detection methods, in particular to a quality detection method for Shuangdong capsules. Background technique [0002] The product of the pharmaceutical composition of the present invention is Shuangdong Capsule, which is a product of Guizhou Yuanyuan Pharmaceutical Co., Ltd., and the product is composed of Gardenia 240g, Astragalus 240g, Hedyotis diffusa 216g, Ophiopogon japonicus 216g, Bitterwood 144g, and Mallow fruit 216g. The preparation method is as follows: the above six flavors, Gardenia, Hedyotis diffusa, and bitterwood are extracted twice with 60% ethanol under reflux, each time for 2 hours, filtered, the filtrate is combined, and the dregs are used for later use; the filtrate is recovered with ethanol and concentrated to 60% Thick paste with a relative density of 1.30-1.35 at ℃, set aside; mix Astragalus, Mallow, Ophiopogon japonicus with the above dregs, add water and decoct twice, each time for 2 h...

AI Technical Summary

Problems solved by technology

[0003] Shuangdong capsules are recorded in YBZ00692012, the original items in the standard are: traits; identification of gardenia, astragalus, hedyotis diffusa, radix radix, and bitterwood TLC; inspection; the content determination item is the determination of geniposide in gardenia; But there are certain defects in the implementation process, such as: identification (1) is the thin-layer identification of gardenia jasminoides in the original standard storage method, the original standard storage method has a large sampling volume and the spot Rf value after spotting is relatively small, which affects Judgment of test results; Identification (2) is the TLC identification of Astragalus membranaceus in the original standard storage method. For the TLC identification of Hedyotis diffusa, the original standard storage method had a larger sampling volume, a larger developer polarity and fewer main spots, which affected the judgment of the test results; identification (4) was the original standard collection method Zhongmai For the TLC identification of winter, the original standard collection method has a small amount of sample, which leads to the inconspicuous main spots, which affects the judgment of the test results; identification (5) is the TLC identification of bitter wood in the original standard collection method, the original standard collection The sampling volume of the method is large, the polarity of the developing agent is large and the main spots are relatively blurred, which affects the judgment of the test results; (6) the determination of gardenia content is recorded in the original standard. Since the extraction solvent of the original standard is methanol ultrasonic extraction for 20 minutes, the extraction The efficiency is low; (7) The content of Astragalus was not detected

[0004] Research on the identification method of Shuangdong Capsules recorded in the prior art (Asia-Pacific Traditional Medicine Volume 11 No. 17 Issue September 2015), the technology recorded in this document is also developed by Guizhou Yuanyuan Pharmaceutical Co., Ltd. TCL identification, TCL identification of astragalus, TCL identification of Ophiopogon japonicus, TCL identification of bitter wood, but there are the following defects in the implementation process: (1) The sampling amount is large and the Rf value of the spots after spotting is small, which affects the test results. Judgment; identification (2) is the TLC identification of Astragalus membranaceus in the original standard recorded prescription, and the spots are blurred after sampling by this method, which affects the judgment of the test results; (3) TLC identification of Hedyotis diffusa is not conducive to Quality control; (4) The TLC identification method of Ophiopogon japonicus has a small sampling volume, which leads to inconspicuous main spots, and the TLC plate is not pre-balanced after sampling, which affects the judgment of the test results; (5) The sampling volume of the bitter wood method Larger, the polarity of the developer is larger and the main spots are blurred, which affects the judgment of the test results; (6) The contents of gardenia and astragalus were not detected

[0005] Application number CN201210374114.3, title of invention: a detection method for capsules for treating lower urinary tract infection, discloses TCL identification of gardenia, TCL identification of astragalus, TCL identification of Ophiopogon japonicus, TCL identification of bitter wood, but in the implementation process There are the following defects in the method: (1) the sampling amount is large and the Rf value of the spot after spotting is small, which affects the judgment of the test result; for the TLC identification of Astragalus membranaceus, the spot after spotting by this method is blurred, which affects the judgment of the test result; (3) For TLC identification of Hedyotis diffusa, the polarity of the developer is large and the main spots are few, which affects the judgment of the test results; Obvious, and the thin-layer plate after spotting the sample is not pre-balanced, which affects the judgment of the test result; (5) the bitter wood method has a large sampling volume, the polarity of the developing agent is large, and the main spots are blurred, which affects the judgment of the test result; (6) ) did not detect the content of Astragalus, and could not effectively control the product quality

Images