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Extraction method of fructus cannabis globulin, product and application thereof

An extraction method, the technology of hemp seed, which is applied in the field of hemp seed globulin extraction, can solve the problems of relatively little research on the extraction process, and achieve the effects of high purity, simple and easy-to-operate extraction method, and avoid loss

Active Publication Date: 2020-11-24
YUNNAN HEMPMON PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the prior art, there are many reports about the functionality of hempseed globulin, but there are relatively few studies on its extraction process. Therefore, a simple and easy to operate, high globulin yield, high purity and good functionality have been developed. The method of extracting edestin is very meaningful

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  • Extraction method of fructus cannabis globulin, product and application thereof
  • Extraction method of fructus cannabis globulin, product and application thereof
  • Extraction method of fructus cannabis globulin, product and application thereof

Examples

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Embodiment 1

[0098] The present embodiment provides a kind of extraction method of hemp seed globulin, described method is as follows:

[0099] (1) carry out dehydration and deoiling treatment to hemp seed, make its moisture content be 8%, oil content rate is 4%;

[0100] (2) Mix 100 g of treated hemp seeds with saline solution at a mass volume ratio of 1:10 g / mL, first heat and extract at 70 °C for 30 min, and then ultrasonically extract at 35 °C for 120 min, with an ultrasonic power of 800 W / L to obtain an extract; the salt solution is a salt solution system composed of 5% sodium chloride, 2% ammonium sulfate, 0.5% hyaluronic acid and 0.5% trehalose.

[0101] (3) filtering the extract obtained in step (2), then centrifuging to obtain a supernatant;

[0102] (4) The supernatant obtained in step (3) was left standing and cooled to 4°C, the protein was precipitated, centrifuged, washed at 4°C with 1% aqueous methylcellulose solution, and then washed with 90% aqueous ethanol at 4°C. After ...

Embodiment 2

[0104] The present embodiment provides a kind of extraction method of hemp seed globulin, described method is as follows:

[0105] (1) carry out dehydration and deoiling treatment to hemp seed, make its moisture content be 5%, oil content rate 5%;

[0106] (2) Mix 100g of processed hemp seeds with saline solution at a mass volume ratio of 1:5g / mL, first heat and extract at 60°C for 40min, then ultrasonically extract at 40°C for 60min, and the ultrasonic power is 800W / L to obtain an extract; the salt solution is a salt solution system composed of 6% sodium chloride, 1% ammonium sulfate and 1% hyaluronic acid.

[0107] (3) filtering the extract obtained in step (2), then centrifuging to obtain a supernatant;

[0108] (4) The supernatant obtained in step (3) was left standing and cooled to 4°C, the protein was precipitated, centrifuged, washed at 4°C with 0.5% aqueous methylcellulose, and then washed with 75% aqueous ethanol at 4°C After washing, 43.7 g of the hemp seed globuli...

Embodiment 3

[0110] The present embodiment provides a kind of extraction method of hemp seed globulin, described method is as follows:

[0111] (1) the hemp seed is carried out dehydration deoiling treatment, makes its water content be 10%, and oil content is 3%;

[0112] (2) Mix 100g of processed hemp seeds with saline solution at a mass volume ratio of 1:15g / mL, first heat and extract at 80°C for 20min, then ultrasonically extract at 30°C for 180min, and the ultrasonic power is 800W / L to obtain an extract; the salt solution is a salt solution system composed of 3% sodium chloride, 4% ammonium sulfate and 1% trehalose.

[0113] (3) filtering the extract obtained in step (2), then centrifuging to obtain a supernatant;

[0114] (4) The supernatant obtained in step (3) was left standing and cooled to 4°C, the protein was precipitated, centrifuged, washed at 4°C with 2% aqueous methylcellulose, and then washed with 95% aqueous ethanol at 4°C After washing, 44.7 g of the hemp seed globulin w...

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Abstract

The invention relates to an extraction method of fructus cannabis globulin, a product and application thereof. The extraction method comprises the following steps: dehydrating and deoiling fructus cannabis, mixing with a salt solution, extracting, filtering and cooling to obtain the fructus cannabis globulin. The extraction method provided by the invention is simple and easy to operate, the extracted globulin is high in yield and high in purity, contains all essential amino acids of a human body, is particularly high in arginine content, can be applied to foods, health products or medicines, and has the effects of resisting fatigue, improving immune health, preventing cardiovascular diseases and the like.

Description

technical field [0001] The invention belongs to the technical field of protein extraction technology, and in particular relates to an extraction method of hemp seed globulin and its product and application. Background technique [0002] Hemp (hemp, thread hemp) belongs to industrial hemp and has a long history of planting in my country. Hemp does not have the value of drug use, and its seeds have high edible value and can also be used as medicine. Hemp seed has the same source of medicine and food, and its medicinal effect was first seen in "Shen Nong's Materia Medica". In recent years, hemp seed has been widely used as health food and clinical medicine in my country. [0003] Hemp seed is rich in oil (30%-50%), the unsaturated fatty acid content contained in oil is higher than 85%, and the ratio of linoleic acid to linolenic acid, omega-6 to omega-3, is between 2.29-4.68 It is beneficial to human health; the main components of human brain tissue are lipids, of which abou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/36C07K1/34C07K1/30A61K38/01A61P39/00A61P9/00
CPCC07K14/415A61K38/011A61P39/00A61P9/00Y02P20/54
Inventor 赵兵徐鹏伟赵庆生袁晓凡常坦然于朝晖
Owner YUNNAN HEMPMON PHARMA CO LTD
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