Primer combination for detecting microdeletion or/and microduplication of recurrent abortion, MLPA probe, gene chip and kit

Abstract

The invention provides a primer combination for detecting microdeletion or / and microduplication of recurrent abortion, an MLPA probe, a gene chip and a kit, and relates to the field of biotechnology.The primer combination has a nucleotide sequence as shown in SEQ ID NO.1-21, can be made into MLPA probes, gene chips, kits or constructs, and recombinant cells, and can also be used as a main component of a system for rapidly screening biological samples susceptible to recurrent abortion. The primer combination provided by the invention can help simultaneously detect recurrent abortion microdeletion or / and microduplication at multiple sites, has good specificity, sensitive response, short detection time and simple method, can detect from point mutation to large chromosome deletion / insertion,has low cost and high clinical application value.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a primer combination for detecting biological samples susceptible to recurrent miscarriage and the MLPA probe, gene chip, kit, system and method prepared therefrom. Background technique

[0002] Recurrent Spontaneous Abortion (RSA) is a common pathological pregnancy in gynecology, which refers to 2 or more consecutive spontaneous abortions. Studies have shown that there are many reasons for recurrent miscarriage, among which genetic factors are closely related to the occurrence of recurrent miscarriage, and chromosomal microdeletions and microduplications are also common types of genetic factors. Chromosomal microdeletion / microduplication refers to the deletion or duplication of 1.5kb-10Mb in length on the chromosome. Although the incidence of each microdeletion or / and microduplication syndrome is very low, due to the limitation of clinical detection technology, a large number of pa...

Images