Ptychidio jordani sperm cryopreservation method

A technology for cryopreservation and jellyfish, which is applied to the preservation, application, and animal husbandry of human or animal bodies. It can solve the problems of rare, frozen jellyfish sperm, etc., and achieves low cost, good cryopreservation effect, Easy and fast effect

Inactive Publication Date: 2020-03-17
GUANGXI UNIV
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there are few methods for long-term cryopreservation and resuscitation of the spermatozoa. Therefore, the establishment of the cryopreservation technology of the spermatozoa is of great significance for the preservation of its excellent germplasm resources and the improvement of artificial reproduction technology.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for cryopreservation of squid sperm, comprising the steps of:

[0022] (1) Semen collection: During the breeding season of the carp, select robust male broodstock with mature glands. After anesthesia, dry the water and mucus around the genital pores of the fish with a dry towel, and then gently squeeze the abdomen along the midline until the genital pores flow out. Milky white semen, suck the semen with a syringe to prevent the mixing of urine, blood and water, inject the collected semen into the cryopreservation tube; take 10 microliters of the collected semen and mix it with an equal volume of potassium chloride with a mass fraction of 0.7%. Observe the movement of sperm under a microscope, and select semen with a sperm viability of more than 90% for dilution and preservation;

[0023] (2) Semen dilution: first mix and dilute the semen collected in step (1) with the diluent at a volume ratio of 1:3 to obtain solution A, and then mix solution A and antifreeze ...

Embodiment 2

[0028] A method for cryopreservation of squid sperm, comprising the steps of:

[0029] (1) Semen collection: During the breeding season of the carp, select robust male broodstock with mature glands. After anesthesia, dry the water and mucus around the genital pores of the fish with a dry towel, and then gently squeeze the abdomen along the midline until the genital pores flow out. Milky white semen, suck the semen with a syringe to prevent the mixing of urine, blood and water, inject the collected semen into the cryopreservation tube; take 10 microliters of the collected semen and mix it with an equal volume of potassium chloride with a mass fraction of 0.7%. Observe the movement of sperm under a microscope, and select semen with a sperm viability of more than 90% for dilution and preservation;

[0030] (2) Semen dilution: first mix and dilute the semen collected in step (1) with the diluent at a volume ratio of 1:5 to obtain solution A, and then mix solution A and antifreeze ...

Embodiment 3

[0035] A method for cryopreservation of squid sperm, comprising the steps of:

[0036] (1) Semen collection: During the breeding season of the carp, select robust male broodstock with mature glands. After anesthesia, dry the water and mucus around the genital pores of the fish with a dry towel, and then gently squeeze the abdomen along the midline until the genital pores flow out. Milky white semen, suck the semen with a syringe to prevent the mixing of urine, blood and water, inject the collected semen into the cryopreservation tube; take 10 microliters of the collected semen and mix it with an equal volume of potassium chloride with a mass fraction of 0.7%. Observe the movement of sperm under a microscope, and select semen with a sperm viability of more than 90% for dilution and preservation;

[0037] (2) Semen dilution: first mix and dilute the semen collected in step (1) with the diluent at a volume ratio of 1:5 to obtain solution A, and then mix solution A and antifreeze ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a Ptychidio jordani sperm cryopreservation method, which comprises four steps, namely semen collection, semen dilution, sperm cryopreservation, and sperm thawing. The semen ofPtychidio jordani is collected through an artificial abdomen squeezing manner, is then mixed with a diluent, and is mixed with an antifreeze agent to obtain a sperm preservation solution, with the diluent being one of a D-17 diluent, a Hank's solution and a Ringer's solution for fish, and the antifreeze agent being one of methanol, dimethyl sulfoxide and dimethyl sulfoxide; then the sperm preservation solution is injected into a cryopreservation tube, and the tube is immersed into liquid nitrogen for long-term storage; when to be in use, the cryopreservation tube containing the sperm preservation solution is taken out of the liquid nitrogen, and then immediately thaw in a water bath with a temperature of 37 DEG C, and the obtained Ptychidio jordani sperms are used for artificial insemination. The method can store the sperms of the Ptychidio jordani for long time and ensures the survival rates of sperms after thawing and resuscitation.

Description

technical field [0001] The invention belongs to the technical field of biological low-temperature cryopreservation, and in particular relates to a method for cryopreservation of jellyfish sperm. Background technique [0002] Shrimp ( Ptychidio jordani ) commonly known as Chia fish and mouse fish, belongs to Cypriniformes, Cyprinidae, Labeoninae, and Ptychidio. Mainly distributed in the Pearl River system in my country, it is a freshwater indigenous fish. The meat of the roll mouth fish is tender. The taste is delicious, and it is also known as the four famous and precious river delicacies of the Pearl River together with the spotted carp, perch and mandarin fish, and has extremely high economic value. [0003] In recent years, with the increasing overfishing and river pollution, the amount of natural resources of cockerel has declined sharply. In addition, people's demand for a new breed of cockerel has continued to expand, and the quality requirements for cockerel seedli...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226A01N1/0284
Inventor 张曼谭虹雨陈秀荔罗成杨春玲王振光杨艳艳
Owner GUANGXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap