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A kind of Streptomyces cellulose fermentation extract and application thereof

A technology of Streptomyces cellulose and extracts, applied in the direction of application, bacteria, fungicides, etc., can solve the problem of restricting the development of Panax notoginseng planting industry, affecting the quality and medicinal value of Panax notoginseng, disease resistance and damage to the micro-ecological balance of farmland And other issues

Active Publication Date: 2021-07-06
YUNNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, fungicidal pesticides such as mancozeb and polyoxin are mainly used to prevent and control Panax notoginseng root rot, but long-term application of chemical pesticides can easily lead to drug resistance of the disease and damage to the micro-ecological balance of farmland. The heavy metal pollution and pesticide residues seriously affect the quality and medicinal value of Panax notoginseng, seriously restricting the development of Panax notoginseng planting industry

Method used

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  • A kind of Streptomyces cellulose fermentation extract and application thereof

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Embodiment 1

[0016] Embodiment 1: Preparation of Streptomyces cellulosus YIM PH20352 fermentation extract

[0017] The bacterial strain used in the present invention is Streptomyces cellulosus YIM PH20352 ( Streptomyces cellulosae YIMPH20352), the strain was deposited in the China Center for Type Culture Collection on April 11, 2017, address: Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M2017188.

[0018] Streptomyces cellulose YIM PH20352 fermentation extract of the present invention obtains through following steps:

[0019] (1) Test tube culture: Streptomyces cellulosus YIM PH20352 ( Streptomyces cellulosae YIM PH20352) strain was inoculated on the slant of the solid medium in the test tube, and cultured at 28°C for 6 days to obtain the test tube species of the strain.

[0020] Among them, the formula of solid medium was: yeast powder 4.0 g; glucose 4.0 g; malt extract 5.0 g; multivitamin 3.5 mg; agar powder 12.0 g; water 1000 mL; pH 7.2.

[0021] (2) Cul...

Embodiment 2

[0025] Example 2: Application of Streptomyces cellulosus YIM PH20352 fermented extract in preparation of anti-ginseng root rot agent

[0026] Indoor toxicity test (inhibition of spore germination method, plate antibacterial test method) was used to test the activity of the fermented extract of Streptomyces cellulosus YIM PH20352 prepared by the above method against the pathogenic fungus of Panax notoginseng root rot.

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Abstract

The invention relates to a fermented extract of Streptomyces cellulose and its application. Streptomyces cellulose YIM PH20352 ( Streptomyces cellulosae YIM PH20352) strain, through 1) cultivating the test tube species of the strain, 2) cultivating the liquid seed of the strain, 3) fermenting the strain and extracting and concentrating to obtain the fermented extract of the strain, the fermented extract has a good effect on the pathogenic fungus of Panax notoginseng root rot The in vitro inhibitory activity can be applied to the preparation of anti-notoginseng root rot agent.

Description

technical field [0001] The invention relates to a fermented extract of Streptomyces cellulose YIM PH20352 and an application thereof, belonging to the technical field of microbial pesticides. Background technique [0002] Panax notoginseng (Panax notoginseng) is a traditional precious Chinese herbal medicine in my country. It prefers to grow in a warm and humid environment, and it is very easy to induce various soil-borne microbial diseases. Root rot is one of the important factors leading to continuous cropping obstacles of Panax notoginseng. At present, fungicidal pesticides such as mancozeb and polyoxin are mainly used to prevent and control Panax notoginseng root rot, but long-term application of chemical pesticides can easily lead to drug resistance of the disease and damage to the micro-ecological balance of farmland. The heavy metal pollution and pesticide residues seriously affect the quality and medicinal value of Panax notoginseng, and seriously restrict the develo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/28A01P3/00C12R1/465
CPCC12N1/20
Inventor 李一青许歆栋赵立兴苗翠苹陈有为
Owner YUNNAN UNIV
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