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Screening method for bacteria for degrading atrazine and cellulose

A technology of atrazine and screening method, which is applied in the field of microorganism separation, and can solve the problems of environmental pollution and waste

Pending Publication Date: 2020-03-20
HUBEI UNIV OF TECH
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Problems solved by technology

At present, natural cellulose is mostly treated by incineration or natural decay, which seriously pollutes the environment and causes great waste

Method used

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  • Screening method for bacteria for degrading atrazine and cellulose

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Embodiment Construction

[0018] In order to better understand the present invention, the invention will be described in detail below in conjunction with the accompanying drawings and specific examples.

[0019] A screening method for bacteria degrading atrazine and cellulose, comprising steps:

[0020] (1) Enrichment culture: Take the forest soil from the Changbai Mountain area of ​​my country that has fallen grass, straw, and fallen leaves all year round, pack it in a sterile bag, take it back and store it in a -20°C freezer for later use, dissolve 1g of the soil in glucose and carboxymethyl The soil suspension was prepared in a mixed aqueous solution of cellulose-based cellulose, and after static incubation at 37°C for 2 hours, the soil suspension was inoculated on the enriched solid medium by the concentration gradient method, and cultivated in an incubator at 28°C for 7 days until monotony appeared. Bacterial colonies, single colonies were transferred to carboxymethyl cellulose solid medium with car...

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Abstract

The invention relates to the technical field of isolation of microbes, in particular to a screening method for bacteria for degrading atrazine and cellulose. The screening method for the bacteria fordegrading the atrazine and the cellulose comprises the steps of (1) taking forest soil covered with vegetation, straw and fallen leaves all year around; inoculating the forest soil onto an enrichmentmedium by employing a concentration gradient method, culturing the inoculated forest soil until single colonies grow out, transferring the single colonies onto a plate taking carboxymethyl cellulose as a unique carbon source for purification until single colonies grow out; (2) performing continuous passage culture on the purified single colonies on the plate taking the carboxymethyl cellulose as the unique carbon source; (3) transferring the single colonies onto a plate taking microcrystalline cellulose powder as a unique carbon source until single colonies grow out, and performing continuouspassage culture; (4) transferring the single colonies onto a culture medium for directed screening of the atrazine through a streak plate method, and performing culture until single colonies grow out;and (5) amplifying 16SrDNA of the single colonies, performing comparison identification on 16SrDNA , and preserving bacterium genera after comparison.

Description

technical field [0001] The invention relates to the technical field of microorganism separation, in particular to a screening method for bacteria degrading atrazine and cellulose. Background technique [0002] Cellulose is a polymer compound synthesized by green plants through photosynthesis and has the largest reserves. With the depletion of fossil fuels and the emergence of environmental pollution problems, the development of natural cellulose has attracted widespread attention of scientists. How to convert natural cellulose into energy that can be used by people has become a new topic that perplexes people. In addition, atrazine (2-chloro-4-diethylamino-6-isopropylamino-1,3,5-triazine) is a triazine herbicide widely used in the world, mainly used in corn and sorghum farmland The removal of weeds has caused soil, surface and groundwater pollution in many countries because of its good leaching property and long-lasting effect. Atrazine strongly interferes with the endocri...

Claims

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Application Information

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IPC IPC(8): C12N1/22C12N1/02
CPCC12N1/22C12N1/02
Inventor 宫春杰王崇鞠
Owner HUBEI UNIV OF TECH
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