A method and device for multifocal super-resolution optical microscopy imaging

Abstract

The invention provides a multi-focus super-resolution optical microscopic imaging method and device. The laser is modulated into excitation light whose light intensity changes periodically according to a sinusoidal function with time, and then the sinusoidally modulated laser is modulated by a spatial light modulator. It can form multiple beams, synchronously scan and excite the imaging sample in parallel, thereby multiplying the scanning efficiency, effectively solving the problem of long imaging time during single-point scanning, and using all the frequency components of the non-sinusoidal fluorescence structured light image to superimpose and reconstruct the sample to be imaged. The resolution of super-resolution images is increased by 3 times compared to the diffraction limit, so the method of this embodiment can realize fast super-resolution two-photon fluorescence microscopy imaging. The method and device disclosed in the present invention can meet the requirement of two-photon fluorescence imaging of tens of nanometers or even higher, and improve the resolution of the two-photon fluorescent structured light image.

Description

technical field [0001] The invention relates to the technical field of optical microscopic imaging, in particular to a multifocal super-resolution optical microscopic imaging method and device. Background technique [0002] Fluorescence microscopy plays a very important role in the field of life science. Among them, two-photon fluorescence microscopy is a new type of nonlinear optical imaging method developed in recent years. It has been widely used in living cells due to its excellent characteristics. , Long-term dynamic three-dimensional imaging of tissue. Two-photon fluorescence microscopy absorbs two photons at the same time, and the emitted fluorescence wavelength is far away from the laser, which can realize dark-field imaging; its technology uses a near-infrared laser source, which can realize deep material tomography in biological tissue; in addition, two-photon fluorescence microscopy Microscopic techniques can avoid the problems of fluorescent photobleaching and p...

AI Technical Summary

Problems solved by technology

[0005] In view of the shortcomings in the above-mentioned prior art, the present invention provides a multi-focus super-resolution optical microscopy imaging method and device to solve the problem of long single-point scanning time, slow imaging speed and low resolution in the existing two-photon SIM technology defects, while solving the problem of low resolution of existing multi-focus microscopy techniques

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