Multi-focus super-resolution optical microscopic imaging method and device

Abstract

The invention provides a multi-focus super-resolution optical microscopic imaging method and a multi-focus super-resolution optical microscopic imaging device. Laser is modulated into exciting light of which the light intensity periodically changes along with time according to a sine function; the light passes through a spatial light modulator; modulating the laser subjected to sinusoidal modulation into a plurality of light beams; synchronously scanning and exciting an imaging sample in parallel. Therefore, the scanning efficiency is improved exponentially; according to the method, the problem of long imaging time during single-point scanning is effectively solved, the super-resolution image of the sample to be imaged is reconstructed by superposing all frequency components of the non-sinusoidal fluorescence structured light image, and the resolution is improved by three times compared with the diffraction limit, so that the method provided by the embodiment of the invention can realize quick super-resolution two-photon fluorescence microscopic imaging. The method and the device disclosed by the invention can meet the requirements of dozens of nanometers or even higher two-photonfluorescence imaging, and improve the resolution of the two-photon fluorescence structured light image.

Description

technical field [0001] The invention relates to the technical field of optical microscopic imaging, in particular to a multi-focus super-resolution optical microscopic imaging method and device. Background technique [0002] Fluorescence microscopy plays a very important role in the field of life sciences. Among them, two-photon fluorescence microscopy is a new nonlinear optical imaging method developed in recent years. Due to its excellent characteristics, it has been widely used in cell biology and living cells. , Long-term dynamic three-dimensional imaging of tissues. Two-photon fluorescence microscopy technology absorbs two photons at the same time, and the emitted fluorescence wavelength is far away from the laser, which can realize dark field imaging; its technology uses a near-infrared laser source, which can realize deep material tomography in biological tissues; in addition, two-photon fluorescence microscopy Microscopy can avoid the problems of fluorescence bleach...

AI Technical Summary

Problems solved by technology

[0005] In view of the shortcomings in the above-mentioned prior art, the present invention provides a multi-focus super-resolution optical microscopy imaging method and device to solve the problem of long single-point scanning time, slow imaging speed and low resolution in the existing two-photon SIM technology defects, while solving the problem of low resolution of existing multi-focus microscopy techniques

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