Method of cultivating tetraploid gingko

Abstract

The invention belongs to the technical field of gingko cultivation and discloses a method of cultivating tetraploid gingko. The method of cultivating tetraploid gingko comprises the following steps ofoverwintering gingko seeds harvested in autumn after stratification; after the gingko seeds are sowed and emerge in spring in a next year, when cotyledons of gingko seedlings are flattened and the tips of true leaves are just exposed, smearing gingko stem tips by a 0.2% colchicines agar sol, wherein the lengths of the stem tips smeared by the colchicines agar sol are smaller than 0.3 cm; removinga solid gel after 12 hours, treating the stem tips once again, and carrying out continuous treatment for 6 times and totally for 72 hours; and obtaining a tetraploid gingko plant after cytological identification. The method can be used for obtaining the tetraploid gingko plant. The polyploid plant has a giant vegetative mass. The method can be used for enlarging germplasm resources of gingko andincreasing genetic diversity to cultivate novel varieties for polyploidy breeding of gingko. By means of polyploidy breeding, a novel gingko variety which is high in growing speed and high in yield can be cultivated.

Description

technical field [0001] The invention belongs to the technical field of ginkgo cultivation, in particular to a method for cultivating tetraploid ginkgo. Background technique [0002] At present, the closest prior art: Ginkgo biloba (Ginkgo biloba L.), also known as ginkgo, is called duck foot tree and gongsun tree in ancient times, belongs to the gymnosperm door, is the only living species of the genus Ginkgo in the Ginkgoaceae family, and is the oldest gymnosperm in the existing gymnosperms. The ancient relict plants are recognized as "living fossils" and are unique multi-purpose economic tree species in my country. [0003] Ginkgo is rich in nutrients, on the one hand it refers to the diversity of nutrients it contains. According to Shandong Academy of Medical Sciences analysis of 100 grams of fresh ginkgo (gingko seeds) kernels produced in Dancheng: 6.96 grams of protein, 1.18 grams of fat, 38.20 grams of sugar, 52 grams of water, 1.47 grams of ash, 1.88 mg of calcium, 2.7...

AI Technical Summary

Problems solved by technology

[0011] In summary, the existing problems in the prior art are: Ginkgo biloba grows slowly, and the sexual reproduction cycle is long, which is the main difficulty in cultivating new varieties through sexual methods. There are few studies in this area by predecessors, especially The polyploid breeding cycle is longer, and there is no report on polyploid induction using the shoot tip of ginkgo seedlings as a material in related research

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