Non-invasive saliva detection method for 1, 5-anhydroglucitol and application of non-invasive saliva detection method

An anhydroglucitol and detection method technology, which is applied in the field of 1,5-anhydroglucitol non-invasive detection of saliva, can solve problems such as poor accuracy of saliva samples, influence on patients' enthusiasm for blood sugar monitoring, patient pain and infection risk, and achieve Avoid pain and infection risk, improve screening efficiency, high patient acceptance effect

Active Publication Date: 2020-04-10
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Abstract
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Problems solved by technology

[0004] However, routine blood-based testing requires invasive venipuncture or acupuncture, which brings pain and infection risks to patients to a certain extent, and will affect the enthusiasm of patients for blood glucose monitoring. Serum 1,5-AG kits are used to detect saliva Sample accuracy is poor

Method used

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  • Non-invasive saliva detection method for 1, 5-anhydroglucitol and application of non-invasive saliva detection method
  • Non-invasive saliva detection method for 1, 5-anhydroglucitol and application of non-invasive saliva detection method
  • Non-invasive saliva detection method for 1, 5-anhydroglucitol and application of non-invasive saliva detection method

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Experimental program
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Embodiment 1

[0031] This embodiment uses a special The establishment of the method of collecting saliva samples through the saliva collection tube.

[0032] Subject selection criteria: (1) aged 18 to 70 years; (2) normal glucose metabolism; (3) no abnormal liver and kidney function; (4) no factors that affect the level of 1,5-AG in the body, such as cystic fibrosis After gastrectomy and subtotal gastrectomy, some traditional Chinese medicines such as Polygala, Polygala, etc. are taken.

[0033] The establishment process is as follows: (1) Firstly, 8 cases of normal glucose metabolism were selected (5 males and 3 females), and three collection methods were planned: chewing cotton swabs 70 times in 1 minute, chewing 70 times in 3 minutes, and containing cotton swabs for 5 minutes. On the first day, the subjects were asked not to brush their teeth in the morning on an empty stomach, and to collect saliva in the three ways 30 minutes after rinsing. On the second day, the subjects were asked ...

Embodiment 2

[0038] This example establishes and evaluates a method for accurately detecting the level of 1,5-AG in saliva by liquid chromatography-mass spectrometry.

[0039] 1. The following describes the selection conditions, clinical information, sample collection and laboratory index detection of the subjects.

[0040] Subject selection criteria: 18 to 70 years old. Exclude those with the following conditions: previous history of diabetes, severe liver and kidney dysfunction, after subtotal gastrectomy, taking certain drugs that affect 1,5-AG levels such as polygala, polygala, etc., cystic fibrosis, taking thyroid hormone , glucocorticoids and sex hormones, acute infection or ketosis, history of pregnancy, tumor, autoimmune disease and mental disease, data missing.

[0041] The present embodiment adopts the diagnostic criteria of the American Diabetes Association (ADA) in 2010 to diagnose diabetes, that is, diabetes is fasting plasma glucose (FPG) ≥ 7.0mmol / L; and / or 2-hour plasma gluc...

Embodiment 3

[0068] This example evaluates the advantages of the method of detecting the level of 1,5-AG in saliva in the screening of diabetes.

[0069] The present invention analyzes the saliva 1,5-AG level of the above 641 cases, and finds the optimal threshold corresponding to the Youden index by using the receiver operating curve (ROC curve) to analyze the saliva 1,5-AG level. The Youden index is As a method of evaluating the authenticity of screening tests, assuming that its false negative (missed diagnosis rate) and false positive (misdiagnosed rate) are equally harmful, the Youden index can be applied. Youden index is the sum of sensitivity and specificity minus 1. Indicates the total ability of the screening method to detect true patients and non-patients. The larger the index, the better the effect of the screening experiment and the greater the authenticity.

[0070] Taking the 2010 ADA as the gold standard for diagnosing diabetes for ROC analysis, fasting saliva 1,5-AG alone ...

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Abstract

On one hand, the invention provides a preparation method for 1, 3-butanediol. A saliva sample of a test individual is collected by using a saliva collection tube and is subjected to liquid chromatography-mass spectrometry analysis, so that the 1, 5-AG level of saliva can be accurately quantified, and pain and infection risks brought to a patient by blood detection and inaccuracy caused by cross reaction of a kit are avoided. On the other hand, the invention provides applicationthereof According to the detected saliva 1, 5-anhydroglucitol level or by combining the saliva 1, 5-anhydroglucitol level with fasting blood glucose or glycosylated hemoglobin, diabetes is screened, the diabetes screening efficiency is improved, and meanwhile, the number of OGTT people can be reduced.

Description

technical field [0001] The invention relates to the field of determination methods of biomarkers, in particular to a saliva non-invasive detection method of 1,5-anhydroglucitol and its application. Background technique [0002] At present, diabetes has developed into a serious global public health problem, bringing a huge economic burden to families and society. Chronic complications of diabetes caused by poor blood sugar control have become an important cause of early death and loss of labor in diabetic patients. According to the assessment of the International Diabetes Federation in 2017, there are about 425 million adults with diabetes aged 20 to 79 in the world, with a prevalence rate of 8.8%. It is estimated that the number of people with diabetes worldwide will reach 629 million in 2045. About 4 million people between the ages of 20 and 79 die from diabetes, which is equivalent to 1 death every 8 seconds. Diabetes accounts for 10.7% of all-cause deaths worldwide, high...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/027G01N2030/062
Inventor 包玉倩贾伟赵爱华周健马晓静王煜非菅朝慧
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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