Micro-fluidic chip immunoassay kit and detection method thereof

A microfluidic chip and immunoassay technology, applied in biological testing, chemical instruments and methods, measuring devices, etc., can solve the problems of high cost, achieve low cost, avoid human errors, and consume less reagents.

Inactive Publication Date: 2020-04-28
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although microfluidic chip manufacturing technology is developing vigorously, most laboratory chips currently need to be driven by electroosmotic drive, heat pump drive or optical capture micropump, and the cost is relatively high.
In addition, various factors such as the material and structure of the chip itself bring certain difficulties to the establishment of immunoassay methods, such as the immobilization, release and flow of immune reagents on the chip. At present, there is no relatively mature application of microfluidic chips Method for rapid detection of veterinary drug multi-residue immunology

Method used

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  • Micro-fluidic chip immunoassay kit and detection method thereof
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  • Micro-fluidic chip immunoassay kit and detection method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0056] A microfluidic chip immunoassay kit, the schematic diagram of which is as follows Figure 1 to Figure 3 As shown, the kit contains a microfluidic chip, including a wafer cover plate 1 and a wafer bottom plate 2; wherein the structural diagram of the wafer cover plate 1 is shown in figure 2 As shown, the structural schematic diagram of the wafer bottom plate 2 is as follows figure 1 shown.

[0057] One or more detection units are arranged in the wafer bottom plate 2, and the structural sequence diagram of a single detection unit is as follows: image 3 As shown; the detection unit is arranged on the radial axis of the disc base plate 2, and is arranged divergently at the center point of the disc base plate 2; the detection unit is arranged in sequence from the circle point to the edge with a liquid storage pool 21, a mixing reaction pool 22, and a detection pool 23 , the waste liquid pool 24 and the first ventilation hole 25, which are connected by microchannels in tu...

Embodiment 3

[0085] A microfluidic chip immunoassay kit prepared in Example 1 is used for detection. In this embodiment, pork is used as the sample to be tested, and the content of chloramphenicol is detected as an example. The specific detection process is as follows:

[0086] 1. Prepare several microfluidic chips for detecting chloramphenicol before the detection.

[0087] 2. Sample processing:

[0088] (1) Three different negative pork samples were selected, and three concentrations in the concentration range of 0.5-15 ng / mL were selected to add chloramphenicol.

[0089] (2) Weigh 2±0.05g homogenized pork samples added with drugs into a 50mL centrifuge tube, add 6mL ethyl acetate, shake and mix well for 3min; centrifuge at room temperature (20℃~25℃) at 4000r for 5min; Blow dry the supernatant in a water bath with nitrogen at 60°C-70°C; reconstitute 1mL of n-hexane, add 1mL of PBS solution, shake fully for 1min; centrifuge at room temperature (20°C-25°C) at 4000r for 5min; take 30μL of su...

Embodiment 4

[0097] A microfluidic chip immunoassay kit prepared in Example 1 is used for detection. In this embodiment, chicken is used as the sample to be tested, and the content of amantadine is detected as an example. The specific detection process is as follows:

[0098] 1. Several microfluidic chips for the detection of amantadine were prepared before the detection.

[0099] 2. Sample processing:

[0100] (1) Three different negative chicken samples were selected, and amantadine was added at three concentrations within the concentration range of 1-20 ng / mL.

[0101] (2) Weigh 2±0.05g homogenized chicken samples added with drugs into a 50mL centrifuge tube, add 6mL acetonitrile, shake and mix well for 3min; centrifuge at room temperature (20℃~25℃) at 4000r for 5min; take 3mL supernatant Blow dry with nitrogen in a water bath at 60°C-70°C; add 1mL of PBS solution and shake fully for 1min; centrifuge at room temperature (20°C-25°C) at 4000r for 5min; take 30μL supernatant for detection...

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Abstract

The invention discloses a micro-fluidic chip immunoassay kit and a detection method thereof. The kit comprises a micro-fluidic chip, a wafer cover plate and a wafer bottom plate, wherein the wafer cover plate and the wafer bottom plate are bonded up and down. One or more detection units are arranged in the wafer bottom plate, and each detection unit comprises a liquid storage tank, a mixed reaction tank, a detection tank, a waste liquid tank and a first vent hole which are connected in sequence; the mixed reaction tank is fixedly provided with a fluorescent probe corresponding to a detection drug; goat anti-mouse immune globulin G and corresponding drug antigens are fixed in the detection tank. The invention also discloses a detection method using the kit. The method has the characteristics of high flux, low sample consumption, high detection speed, simplicity and convenience in operation and the like; the method has the advantages of no need of an electrode, a voltage or an external magnetic field, no need of electroosmosis driving, hot air pump driving or optical capture micropump driving, simultaneous detection of various veterinary drug residues in various matrixes, suitableness for on-site rapid screening analysis, and great alleviation of the detection pressure of related detection personnel.

Description

technical field [0001] The invention relates to the technical field of rapid detection of food safety, more specifically, to a microfluidic chip immunoassay kit and a detection method thereof. Background technique [0002] Veterinary drug residues refer to drug prototypes, metabolites and drug impurities that accumulate or store in animal cells, tissues or organs after administration of drugs to animals. Veterinary drugs play a very important role in preventing and controlling animal diseases, improving production efficiency, and improving the quality of animal products. And the use of counterfeit and inferior drugs has caused the problem of veterinary drug residues to be very serious. Residues of harmful substances in food of animal origin not only cause direct harm to human life and health, but also greatly endanger the development of animal husbandry and the ecological environment. [0003] Veterinary drug residues mainly include growth-promoting, antibiotic and antibac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/58G01N33/53B01L3/00
CPCB01L3/5027G01N33/5304G01N33/582G01N33/94G01N33/9446
Inventor 杨金易吴颖曾道平苏晓娜沈玉栋王弘陈丽韦田
Owner SOUTH CHINA AGRI UNIV
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