Domestication method of nuclear polyhedrosis virus adaptable to diaphania perspectalis and application

A technology of nuclear polyhedrosis and Euonymus chinensis, which is applied in the field of domestication and preparation of nuclear polyhedrosis virus, can solve the problems of being unable to adapt to pest outbreaks and control, high cost, time-consuming and low efficiency, and achieve easy infection , save time, good use effect
CN111117971APending Publication Date: 2020-05-08SUZHOU MIDI BIOTECH CO LTD +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SUZHOU MIDI BIOTECH CO LTD
Publication Date
2020-05-08

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Abstract

The invention discloses a domestication method of a nuclear polyhedrosis virus adaptable to diaphania perspectalis and application. The domestication method comprises the following steps: diluting thenuclear polyhedrosis virus with water for 10-20 times, and then applying the diluted nuclear polyhedrosis virus to buxus sinica branches and leaves to obtain virus-containing feed; firstly, treatingdiaphania perspectalis larvae with matrine, then, feeding the hungry diaphania perspectalis larvae with the virus-containing feed, collecting virus-infected larvae or dead larvae, carrying out crushing and filtration, centrifuging a filtrate, and performing collection and degermining to obtain a domesticated free nuclear polyhedrosis virus; taking the free nuclear polyhedrosis virus as a virus source and repeating the previous steps to increase the domestication generation; carrying out culture and amplification on the domesticated free nuclear polyhedrosis virus to obtain the nuclear polyhedrosis virus. The nuclear polyhedrosis virus has higher killing performance on the diaphania perspectalis and has the characteristics of high target, long control time and low probability of productionof drug resistance of pests, and the domestication success rate of the method is high.
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Description

technical field

[0001] The invention relates to a domestication and preparation method of a nuclear polyhedrosis virus, in particular to a method for domestication and industrial production of a nuclear polyhedrosis virus suitable for the yellow poplar silk borer and its application, belonging to the technical field of biological pesticides. Background technique

[0002] Diaphania perspectalis (Walker), an insect belonging to the genus Diaphania perspectalis of the family Lepidoptera, is widely distributed. It mainly harms Buxuaceae plants, such as Buxus melon, Buxus Buxus, Buxus euphratica, Buxus microphylla, Buxus korean, holly, euonymus, etc. Among them, Buxus melon seeds and Buxus Buxus suffer the most. The young shoots and leaves are eaten by larvae, and they often spin silk to bind the leaves and feed on them. The damaged leaves are scorched, and the rate of damaged plants in serious streets is more than 50%, even up to 90%. When an outbreak occurs, the leaves can be e...

Claims

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