Domestication method of nuclear polyhedrosis virus adaptable to diaphania perspectalis and application

A technology of nuclear polyhedrosis and Euonymus chinensis, which is applied in the field of domestication and preparation of nuclear polyhedrosis virus, can solve the problems of being unable to adapt to pest outbreaks and control, high cost, time-consuming and low efficiency, and achieve easy infection , save time, good use effect

Pending Publication Date: 2020-05-08
SUZHOU MIDI BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The current sources of insect viruses are mainly found and collected in the wild, but this method is expensive, ti

Method used

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  • Domestication method of nuclear polyhedrosis virus adaptable to diaphania perspectalis and application
  • Domestication method of nuclear polyhedrosis virus adaptable to diaphania perspectalis and application
  • Domestication method of nuclear polyhedrosis virus adaptable to diaphania perspectalis and application

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preparation example Construction

[0027] In view of the technical problems existing in the prior art, the inventor of this case has been able to propose the technical scheme of the present invention after long-term research and a large amount of practice, which is mainly applicable to the indoor domestication and preparation method of the nuclear polyhedrosis virus of the yellow poplar silk borer. The dosage form of the biopesticide prepared by the method is suspension concentrate, and the active ingredient is nuclear polyhedrosis virus. The method specifically includes the steps of virus domestication, collection and amplification of nuclear polyhedrosis virus, preparation of dosage form and the like.

[0028] The technical solution, its implementation process and principle will be further explained as follows.

[0029] One aspect of the embodiments of the present invention provides a method for acclimating nuclear polyhedrosis virus adapted to the yellow poplar silk borer, which is through indoor cultivation...

Embodiment 1

[0075] Domestication of Spodoptera litura nuclear polyhedrosis virus:

[0076] The name of the biopesticide virus that is applied to the prevention and control of the box borer is Spodoptera litura nucleopolyhedrosis virus, and the domestication of the virus and the preparation method of the biopesticide include the following steps:

[0077] (1) The initial infection of the virus: a large number of larvae of Euonymus chinensis are raised, and the commercially available Spodoptera litura nucleopolyhedrosis virus pesticide is diluted with sterile water (1:20) and then soaked in fresh branches and leaves of Euonymus microphylla, and dried in the shade Water, select the 3-4 instar larvae of Euonymus chinensis, first treat the larvae of Euonymus chinensis with matrine, and then starve for a period of time, usually 12-24 hours, feed the treated branches and leaves of Euonymus microphylla, etc. After eating the feed, feed the normal branches and leaves, observe and collect the poison...

Embodiment 2

[0082] Domestication of beet armyworm nuclear polyhedrosis virus:

[0083] The name of the biopesticide virus applied to the prevention and control of the yellow poplar silk borer is the beet armyworm nuclear polyhedrosis virus, and the domestication of the virus and the preparation method of the biopesticide include the following steps:

[0084] (1) The initial infection of the virus: a large number of larvae of Euonymus japonicus were raised, and the commercially available pesticide of beet armyworm nuclear polyhedrosis virus was diluted with sterile water (1:10) and then soaked in fresh branches and leaves of Euonymus microphylla , dry the water in the shade, select the 3-4 instar larvae of Euonymus chinensis, and first treat the larvae of Euonymus spp. After eating the poisoned feed, feed the normal branches and leaves, observe and collect the poisoned dead or dying larvae, after grinding in a mortar, filter with 4 layers of gauze, centrifuge, collect the supernatant, ster...

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Abstract

The invention discloses a domestication method of a nuclear polyhedrosis virus adaptable to diaphania perspectalis and application. The domestication method comprises the following steps: diluting thenuclear polyhedrosis virus with water for 10-20 times, and then applying the diluted nuclear polyhedrosis virus to buxus sinica branches and leaves to obtain virus-containing feed; firstly, treatingdiaphania perspectalis larvae with matrine, then, feeding the hungry diaphania perspectalis larvae with the virus-containing feed, collecting virus-infected larvae or dead larvae, carrying out crushing and filtration, centrifuging a filtrate, and performing collection and degermining to obtain a domesticated free nuclear polyhedrosis virus; taking the free nuclear polyhedrosis virus as a virus source and repeating the previous steps to increase the domestication generation; carrying out culture and amplification on the domesticated free nuclear polyhedrosis virus to obtain the nuclear polyhedrosis virus. The nuclear polyhedrosis virus has higher killing performance on the diaphania perspectalis and has the characteristics of high target, long control time and low probability of productionof drug resistance of pests, and the domestication success rate of the method is high.

Description

technical field [0001] The invention relates to a domestication and preparation method of a nuclear polyhedrosis virus, in particular to a method for domestication and industrial production of a nuclear polyhedrosis virus suitable for the yellow poplar silk borer and its application, belonging to the technical field of biological pesticides. Background technique [0002] Diaphania perspectalis (Walker), an insect belonging to the genus Diaphania perspectalis of the family Lepidoptera, is widely distributed. It mainly harms Buxuaceae plants, such as Buxus melon, Buxus Buxus, Buxus euphratica, Buxus microphylla, Buxus korean, holly, euonymus, etc. Among them, Buxus melon seeds and Buxus Buxus suffer the most. The young shoots and leaves are eaten by larvae, and they often spin silk to bind the leaves and feed on them. The damaged leaves are scorched, and the rate of damaged plants in serious streets is more than 50%, even up to 90%. When an outbreak occurs, the leaves can be e...

Claims

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Application Information

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IPC IPC(8): C12N7/00A01N63/40A01P7/04C12R1/93
CPCC12N7/00A01N63/00C12N2710/14151C12N2710/14131C12N2710/14171
Inventor 孙祥明滕小锘袁秀丽金亮
Owner SUZHOU MIDI BIOTECH CO LTD
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