Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting mycotoxin pollution in agricultural products

A mycotoxin and detection method technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of the influence of detection results and less extraction amount, and achieve the effect of reducing detection cost, good effect, and increasing the area of ​​action

Active Publication Date: 2020-05-08
通标标准技术服务(天津)有限公司
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the extraction of mycotoxins in the sample has a major impact on the subsequent detection. Inaccurate selection of the polarity of the solvent will result in less extraction, or more protein or starch impurities in the agricultural product itself, both of which are harmful to the detection. Results have high impact, so there is a need for an effective extraction assay for mycotoxins

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting mycotoxin pollution in agricultural products
  • Method for detecting mycotoxin pollution in agricultural products
  • Method for detecting mycotoxin pollution in agricultural products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A detection method for mycotoxin contamination in agricultural products, the detection method comprising the following steps:

[0032] Step 1. Preparation of the adsorption phase. Mix and stir the bog iron ore and activated carbon according to the weight ratio of 1:5 to obtain the adsorption phase. The adsorption phase is soaked in a phosphate buffer solution with a pH value of 6 to 8 to obtain The adsorption phase is pressed into a tablet with a thickness of 2 to 5 cm, and wrapped with microfiber filter cloth to obtain the finished product of the adsorption phase;

[0033] The preparation of the sample is to weigh the sample of the agricultural product to be tested and pulverize it. The sample is covered with a microfiber filter cloth, and then the adsorption phase and the sample are sandwiched. The weight of the adsorption phase used is 10% of the weight of the sample to be tested. Times, to obtain the extraction sample, compaction, binding and fixing;

[0034] Step ...

Embodiment 2

[0045] A detection method for mycotoxin contamination in agricultural products, the detection method comprising the following steps:

[0046] Step 1, the preparation of the adsorption phase, mix and stir the bog iron ore, artificial zeolite or diatomaceous earth according to the weight ratio of 1:5:2 to obtain the adsorption phase; the artificial zeolite is passed through benzyl dimethyl Octadecyl ammonium chloride hydrate is soaked for 15-30 minutes, and the quality of benzyldimethyloctadecyl ammonium chloride hydrate used is 1.5 times that of artificial zeolite; The phosphate buffer solution with a value of 6-8 is soaked for 5-10 minutes; the obtained adsorption phase is pressed into a tablet with a thickness of 2-5 cm, and wrapped with a microfiber filter cloth to obtain the finished product of the adsorption phase;

[0047] The preparation of the sample, the sample of the agricultural product to be tested is weighed and then crushed, and the sample is covered with a microf...

Embodiment 3

[0056] A detection method for mycotoxin contamination in agricultural products, the detection method comprising the following steps:

[0057] Step 1. Preparation of the adsorption phase. For the preparation of the adsorption phase, the bog iron ore and activated carbon are mixed and stirred evenly according to the weight ratio of 1:5 to obtain the adsorption phase, and the adsorption phase is treated with a phosphate buffer solution with a pH value of 6-8 Soaking treatment, pressing the obtained adsorption phase into a tablet with a thickness of 2 to 5 cm, and wrapping it with microfiber filter cloth to obtain the finished adsorption phase;

[0058] The preparation of the sample, the sample of the agricultural product to be tested is weighed and then pulverized, and the sample is covered with a microfiber filter cloth, and then the adsorption phase is sandwiched with the sample, and the weight of the adsorption phase used is 10% of the weight of the sample to be tested. Times,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for detecting mycotoxin pollution in agricultural products. The method comprises the following steps: preparation, extraction, desorption and detection of an adsorbedphase. According to the invention, aluminosilicate and activated carbon are adopted as raw materials, and an adsorbed phase is prepared. Through adoption of a mode of extraction, adsorption and then desorption, the situation that a detection result is deviated due to the fact that the extracted mycotoxin content is low or other impurities of the sample are mixed when the mycotoxin content of an agricultural product sample is detected is reduced; detection precision is improved, in addition, a sample and the adsorbed phase are clamped; compared with the existing operation of directly mixing a sample with an adsorbed phase, the operation is simple; the adsorbed phase can be repeatedly used, the sample can be recovered, the desorption operation is performed by using a desorption apparatus, and the mycotoxin adsorbed on the adsorbed phase is subjected to pressurization desorption by using the evaporation expansion pressure of a desorption liquid, such that the desorption effect is completecompared to the desorption effect of an existing atmospheric pressure heating soaking method, the use amount of the desorption liquid is less, and the subsequent detection precision is indirectly improved.

Description

technical field [0001] The invention relates to the field of mycotoxin detection. More specifically, the present invention relates to a method for detecting mycotoxin contamination in agricultural products. Background technique [0002] Mycotoxins are secondary metabolites produced by fungi. They are widely found in agricultural products such as grain and oil, and can cause fatal results to both humans and animals. There is not a one-to-one correspondence between the types of fungi and the types of toxins. There will be a Fungi can produce multiple toxins, and the toxicity of mycotoxins has an additive effect, and the toxicity of mycotoxins also varies greatly, depending on the type / amount / intake amount of the toxin, the duration of ingestion, environmental factors, etc. The more harmful mycotoxins mainly include aflatoxin, zearalenone, deoxynivalenol, ochratin A, fumonisins, T2 / HT2 toxins, etc. [0003] For the above reasons, the detection of mycotoxins in agricultural pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/06G01N30/36G01N30/72G01N30/90G01N30/95
CPCG01N30/02G01N30/06G01N30/36G01N30/7266G01N30/90G01N30/95G01N2030/027
Inventor 郭林海李骞赵春平朱体政苏璇
Owner 通标标准技术服务(天津)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com