Automatic nucleic acid extraction and real-time quantitative PCR device and matched reaction box

Abstract

The invention discloses an automatic nucleic acid extraction and real-time quantitative PCR device and a matched reaction box. The device consists of the matched reaction box, a light source and fluorescence receiving device, a heating-cooling device, a matched reaction box supporting structure, a motor set and ultrasonic amplitude transformer and a controller, wherein the matched reaction box mainly comprises a main body, a top cover, a reaction chamber, an injection syringe and the like; the light source and fluorescence receiving device mainly comprises light-emitting diodes, lenses, lightfilters, photodiodes and the like; the heating-cooling device mainly comprises semiconductor coolers, heating sheets, heat-dissipation wires and temperature inductors; and the motor set and ultrasonicamplitude transformer mainly comprises a motor, a lead screw, an ultrasonic amplitude transformer and the like. According to the automatic nucleic acid extraction and real-time quantitative PCR device, automatic closed nucleic acid extraction and amplification detection can be realized through a commercialized nucleic acid extraction detection regent, so that the labor force is greatly saved, meanwhile, the nucleic acid pollution probability is greatly decreased, and risks of biohazards such as aerosol are greatly decreased; and the whole device and the matched reaction box are small in volume and can be applied to nucleic acid experiments in various scenes.

Description

technical field [0001] The invention belongs to the field of biomedical equipment, and in particular relates to a computer-controlled automatic nucleic acid extraction and real-time quantitative PCR device and a matching reaction box for automatic processing and detection of nucleic acid samples. Background technique [0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific nucleic acid fragments. Since the 1980s, it has been widely used in clinical and scientific research of nucleic acid and drug resistance sites. At present, on the basis of polymerase chain reaction (PCR), derivative technologies such as endpoint PCR, real-time quantitative PCR (qPCR), digital PCR (dPCR) and nucleic acid constant temperature amplification reactions based on different principles have been developed. [0003] Among them, the end-point PCR is the traditional PCR itself cannot quantitatively detect nucleic acid; digital PCR divides the reaction system i...

AI Technical Summary

Problems solved by technology

In recent years, instruments and reagents for nucleic acid extraction and detection have also emerged. However, due to the preciousness of nucleic acid samples, the extraction and detection instruments are large, the consumables are relatively expensive, and special experimental sites are required. The experimental process is relatively complicated and time-consuming, and the experimental process is prone to accidents. Pollution, nucleic acid sample processing and transfer process are prone to aerosol hazards and other reasons, the demand for low-cost automatic nucleic acid extraction and detection instruments and consumables based on real-time quantitative PCR technology has become more and more urgent

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