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Method for creating HMW-GS (high molecular weight glutenin subunits) fully deleted waxy wheat

A technology of HMW-GS, waxy wheat, applied in the field of plant biology, can solve the problems of wet gluten content, formation time and stabilization time decrease, etc.

Pending Publication Date: 2020-05-22
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The results showed that the HMW-GS total deficiency line can lead to a significant decrease in wet gluten content, development time and stability time

Method used

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  • Method for creating HMW-GS (high molecular weight glutenin subunits) fully deleted waxy wheat
  • Method for creating HMW-GS (high molecular weight glutenin subunits) fully deleted waxy wheat
  • Method for creating HMW-GS (high molecular weight glutenin subunits) fully deleted waxy wheat

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Embodiment 1

[0162] The creation process of HMW-GS full deletion waxy wheat of the present invention figure 1 . specifically:

[0163] Step 1: Using the Glu-1 site to express the hexaploid common wheat HMW-GS of the Null type HMW-GS (that is, 0) with a complete deletion of wheat G 0 It is the female parent (this female parent material is obtained according to the creation method of a kind of null type HMW-GS wheat in CN 108432630A, the public can obtain it from Sichuan Agricultural University, and the applicant guarantees to distribute the material to the public within 20 years from the date of application, of course, also HMW-GS complete deletion wheat in other prior art can be used as the female parent), waxy wheat Chuan 13015 as the male parent (this material is selected from the participating lines of the regional wheat variety test in Sichuan Province through open channels, and the provider is Sichuan Zhu Huazhong, Crop Research Institute of Sichuan Academy of Agricultural Sciences,...

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Abstract

The invention discloses a method for creating HMW-GS (high molecular weight glutenin subunits) fully deleted waxy wheat. The method comprises the following steps of step (1), carrying out artificial hybridization of the HMW-GS fully deleted wheat and waxy wheat to obtain a hybrid F1 seed; step (2), planting the hybrid F1 seed, performing selfing and harvesting an F2 seed and dividing the F2 seed into single grains, and screening the single-grain waxy wheat of which three Wx genetic loci do not express the Waxy protein; scraping the endosperm powder of the single-grain waxy wheat for SDS-PAGE identification of the HMW-GS composition, and screening the single seed that does not express any HMW-GS, and the single seed is the HMW-GS fully deleted waxy wheat; and step (3), planting the single seed of the HMW-GS fully deleted waxy wheat, performing selfing and harvesting an F3 seed, and continuously planting in single grains and performing selfing to obtain F5, planting in individual plants,and determining a quality index after F6 is stable.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a method for creating HMW-GS complete deletion waxy wheat which does not exist in nature. Background technique [0002] The composition and content of starch and high molecular weight glutenin subunits (HMW-GS) in wheat endosperm are important factors affecting the processing quality of wheat flour. [0003] The starch content in the endosperm of common wheat accounts for about 70% of the total weight of the grain. It is divided into two types: linear chain and branched chain. The proportion of the two in the total starch is relatively stable, about 25% and 75% (Zhai Hongmei et al., 2008). Wheat granule starch-binding enzyme I, also known as GBSSI, directly determines the synthesis, content and amylose / amylose ratio of wheat amylose (Vrinten and Nakamura 2000). The gene encoding the enzyme protein is also called Wx, located at the Wx-A1, Wx-B1 and Wx-D1 sites on 7A...

Claims

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Application Information

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IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 颜泽洪代寿芬刘登才刘刚李健魏育明郑有良
Owner SICHUAN AGRI UNIV
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