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Application of soybean protein gmubca and related biomaterials related to seed weight regulation
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A biomaterial and recombinant microorganism technology, which is applied in the application field of soybean protein GmUBCa and related biomaterials related to seed weight regulation, can solve the problems of inability to meet national needs and backwardness.
Active Publication Date: 2022-03-01
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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Problems solved by technology
Soybean production lags far behind the pace of development of other domestic food crops and cannot meet national needs
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Embodiment 1
[0053] Embodiment 1, be derived from the cDNA clone of the protein GmUBCa gene (GmUBCa) related to seed weight of soybean and the construction of plant expression vector
[0054] The inventors of the present invention obtained a highly expressed gene GmUBCa during transcriptome analysis of soybean seed development. The function of the gene was tested. The transcription level of GmUBCa was the highest in soybean flowers, followed by seeds and roots, very low in seedlings and pods, and very low in leaves ( figure 1 ).
[0055] The total RNA of HN44 seedlings was extracted, and the RNA was reverse-transcribed with reverse transcriptase to synthesize cDNA.
[0056] According to the information of GmUBCa full-length cDNA sequence in the soybean genome sequence of PlantGDB and the determined HN44 genome sequence, primers were designed, and the primer sequences were as follows:
[0057] GmUBCa-up: 5'-ATGGCCTCCAAGCGGATT
[0058] GmUBCa-dp: 5'-CTAGCCCATTGCATACTTCTGAGTC
[0059] Us...
Embodiment 2
[0061] Embodiment 2, the acquisition of GmUBCa overexpression Arabidopsis
[0062] 1. Obtaining recombinant Agrobacterium
[0063] The recombinant vector pGWB411-GmUBCa containing GmUBCa obtained in Example 1 was introduced into Agrobacterium GV3101 by electric shock method to obtain recombinant Agrobacterium containing pGWB411-GmUBCa, which was named recombinant Agrobacterium
[0064] GV3101 / GmUBCa.
[0065] 2. Acquisition and identification of transgenic GmUBCa Arabidopsis
[0066] The recombinant Agrobacterium GV3101 / GmUBCa was cultivated to the logarithmic phase, and then transformed into Arabidopsis thaliana (Col-0) (seeds from Arabidopsis Biological Resource Center (ABRC)) by vacuum method, and the seeds were harvested after cultivation (T 1 generation), the seeds were sown on the MS screening medium containing kanamycin (50mg / L), and the T 1 When the generation plant grows to 4-6 leaves, it is moved to the vermiculite to grow, and the harvest T 1 Generation single ...
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Abstract
The invention discloses the application of soybean protein GmUBCa related to seed weight regulation and related biological materials. The application is the application of GmUBCa or its related biological materials in regulating the grain weight of seed plants; the GmUBCa is a protein with an amino acid sequence as shown in SEQ ID No.2; the biological materials are any of the following: B1) The nucleic acid molecule encoding the GmUBCa; B2) the expression cassette containing the nucleic acid molecule of B1); B3) the recombinant vector containing the nucleic acid molecule of B1), or the recombinant vector containing the expression cassette of B2); B4) the expression cassette containing B1 ) the recombinant microorganism of the nucleic acid molecule, or the recombinant microorganism containing the expression cassette of B2), or the recombinant microorganism containing the recombinant vector of B3); B5) the molecular marker of the GmUBCa gene. GmUBCa and its related biomaterials can be used to increase crop yields and breed high-yielding varieties.
Description
technical field [0001] The invention relates to the application of soybean protein GmUBCa and related biological materials related to seed weight regulation in the field of molecular biology. Background technique [0002] Soybean is an important traditional crop with rich nutritional value. It is an important economic crop to provide grain, oil and feed. Soybean oil also has many uses in industrial production, such as biofuel, surfactant, softener, etc. China used to be the world's largest soybean producer. However, in recent years, my country's soybean production has only accounted for a quarter of demand, making China the world's largest soybean importer, with total imports accounting for half of the world's total soybean exports. Soybean production lags far behind the pace of development of other domestic food crops and cannot meet national needs. In the past 50 years, soybean production has only increased by 62%, while other food crops have increased by more than 5 time...
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