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ZmAER protein and coding gene and application thereof

A technology that encodes genes and proteins, applied in the fields of biotechnology and plant genetics and breeding, can solve problems such as the destruction of biological macromolecular structures and cell dysfunction

Active Publication Date: 2019-09-20
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These lipid-derived active carbonyl compounds are rich in electrophilic groups, which will attack biomacromolecules in cells, proteins, DNA, RNA, and nucleophilic groups in cell membranes, resulting in the destruction of biomacromolecular structures, which in turn makes cells dysfunction

Method used

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  • ZmAER protein and coding gene and application thereof
  • ZmAER protein and coding gene and application thereof
  • ZmAER protein and coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1. Acquisition of ZmAER and its coding gene

[0076] The total RNA from multiple tissues of corn inbred line B73-329 was extracted and reverse transcribed into cDNA. After a lot of sequence analysis, expression analysis and functional verification, a DNA coding sequence was found from cDNA, as shown in sequence 2 of the sequence list, and the encoded protein is shown in sequence 1 of the sequence list.

[0077] The protein shown in sequence 1 of the sequence listing is named ZmAER protein. The gene encoding the ZmAER protein is named ZmAER gene, and its open reading frame is shown in Sequence 2 of the Sequence Listing.

Embodiment 2

[0078] Example 2. Analysis of the tissue expression pattern of ZmAER

[0079] 1. Select seeds of corn inbred line B73-329 with full grains and soak them in 10% H 2 O 2 Sterilize for 30 minutes to ensure that the seeds are fully exposed to the disinfectant.

[0080] 2. After completing step 1, rinse the seeds with pure water 3-5 times until they are clean, and then soak the seeds in pure water overnight.

[0081] 3. After completing step 2, spread the seeds flat in a petri dish with moist filter paper, and germinate in the dark until the whiteness is about 0.5 cm.

[0082] 4. After completing step 3, sown the dew-white seeds in vermiculite to germinate until two leaves grow.

[0083] After completing step 4, select one-leaf and one-heart seedlings with consistent growth, carefully remove the endosperm and embryo, and wash off the vermiculite attached to the roots of the seedlings with deionized water, and apply 1‰ carbendazim solution to the wound of the de-embryo, and ensure the roots o...

Embodiment 3

[0117] Example 3. Phenotype identification of ZmAER overexpression strains under low nitrogen conditions under hydroponic conditions

[0118] Plants to be tested: T3 generation positive transgenic ZmAER maize homozygous lines (OE1, OE3, OE4, OE6, OE7, OE8), T3 generation positive transgenic vector maize homozygous lines and maize inbred lines B73-329.

[0119] Follow steps 1-5 of Example 2 to operate. Observe the plant phenotype and leaf position phenotype, count the biomass of the shoots and roots, and determine the nitrogen content and nitrogen use efficiency of the plant.

[0120] Six strains were tested for each strain.

[0121] The nitrogen content determination method is as follows: a fresh corn plant sample is dried at 105°C and then dried at 70°C to a constant weight, and the dry weight of each part is measured. After being crushed by the pulverizer, use a one-ten thousand balance to weigh 0.1g dry sample in the digestive tube, and use H 2 SO 4 -H 2 O 2 The method is used for...

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Abstract

The invention discloses ZmAER protein and a coding gene and application thereof. The invention claims ZmAER protein or application its related biomaterial in regulating plant tolerance to low nitrogen. Olefine aldehyde oxidoreductase coded via corn ZmAER gene, provided herein, can regulate the content of active carbonyl compounds to affect the tolerance to low nitrogen; a new idea can be provided for studying nitrogen-efficient mechanisms of crops; therefore, a new strategy is provided for the cultivation of nitrogen-efficient varieties.

Description

Technical field [0001] The invention relates to the fields of biotechnology and plant genetic breeding, in particular to a ZmAER protein and its coding gene and application. Background technique [0002] Nitrogen is a macronutrient element necessary for plant growth and development, and it is also one of the main limiting factors affecting crop growth and yield. Nitrogen deficiency not only seriously affects the growth of roots, but also affects biochemical indicators such as chlorophyll content, nitrate reductase and glutamate synthase activities, which in turn affects the photosynthetic efficiency of plants, and ultimately leads to a decrease in yield. [0003] Studies have shown that non-legume crops need to absorb 20 to 50 grams of nitrogen for every kilogram of dry matter produced. Therefore, the natural supply of nitrogen in the soil under most agricultural planting systems is far from sufficient for the growth of crops. The application of nitrogen fertilizer and the selecti...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8271
Inventor 王喜庆王祎刘芳
Owner CHINA AGRI UNIV
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