Application of miR156 and related biomaterial thereof in regulating disease resistance of plants

A biomaterial and disease resistance technology, applied in the application field of regulating plant disease resistance, can solve problems such as unreported research, and achieve the effect of enriching forest tree breeding resources

Pending Publication Date: 2019-10-15
NORTHEAST FORESTRY UNIVERSITY
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  • Application of miR156 and related biomaterial thereof in regulating disease resistance of plants
  • Application of miR156 and related biomaterial thereof in regulating disease resistance of plants
  • Application of miR156 and related biomaterial thereof in regulating disease resistance of plants

Examples

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Embodiment 1

[0066] Example 1, the application of miR156 in regulating the disease resistance of birch

[0067] 1. Construction and identification of transmiR156 birch

[0068] The Gateway technology was used to construct the overexpression vector pGWB2-miR156, and the genetic transformation of birch was carried out by using the zygotic embryo method mediated by Agrobacterium tumefaciens. The miR156-transformed birch was identified by PCR and quantitative PCR. Specific steps are as follows:

[0069] 1. The construction of miR156 birch

[0070] (1) Construction of overexpression vector pGWB2-miR156

[0071] The 192bp DNA fragment containing the mature sequence of miR156 (the DNA fragment shown in the 221-412 position of sequence 3) was constructed into the entry vector pENTR (purchased from Invitrogen Company) by Gateway technology, and then used GatewayLR Clonase™ II Enzyme Mix (purchased from Invitrogen Co., Ltd.) carried out homologous recombination of a DNA fragment with a size of 19...

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Abstract

The invention discloses miR156 and application of a related biomaterial thereof in regulating disease resistance of plants. Transgenic miR156 white birch is used as a research object, wild type whitebirch serves as a control, the activity of superoxide dismutase, catalase, peroxidase, polyphenol oxidase and phenylalanine ammonialyase is determined by detecting the speck number after diseases occur, and the effects of miR156 on damage degree of disease infected plants is researched in the physiological aspect; by analyzing the transgenic miR156 white birch and disease-resistant related genes in the control, regulation of miR156 on disease resistant ways of plants is further proved. The miR156 has great significance in disclosing disease resistant functions of the miR156 and breeding disease-resistant white birch varieties and contributes to enrichment of forest tree breeding resources.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of miR156 and related biological materials in regulating plant disease resistance. Background technique [0002] MicroRNA (miRNA) is a kind of non-coding small RNA (Small RNA). MicroRNA was first discovered in 1993, in Caenorhabditis elegans, the first gene lin-4 that can temporally regulate embryonic development was discovered. In 2000, it was discovered in nematodes that regulate the transition from late larvae to adults The gene let-7, and this kind of small molecule RNA with a length of about 21nt was named stRNA (small temporal RNA). With the continuous development of molecular cloning technology and bioinformatics, researchers subsequently discovered different types and quantities of small RNAs in plants such as Arabidopsis thaliana, wheat (Triticum aestivum) and rice (Oryza sativa), And it is called MicroRNA (miRNA). In plants, this type of RNA ma...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/82C12N5/10A01H5/00A01H6/00
CPCC12N15/113C12N15/8279C12N2310/141Y02A40/146
Inventor 李慧玉董京祥姜静刘桂丰李孟卓颜斌
Owner NORTHEAST FORESTRY UNIVERSITY
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