Method for culturing cauliflower regenerative tree by free microspore culture technology

A technology for microspore culture and regeneration of plants, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve problems such as stagnant development of microspore embryos, achieve the goals of shortening breeding time, enriching breeding resources, and speeding up the pace of breeding Effect

Inactive Publication Date: 2004-12-08
TIANJIN RES INST OF VEGETABLE
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Problems solved by technology

[0004] The object of the present invention is to provide a method for obtaining regenerative plants of cauliflower using free microspore culture technology, which solve

Method used

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Example Embodiment

[0008] A method for cultivating cauliflower using free microspore culture technology is carried out according to the following steps: ① Take materials: take the inflorescence with the bud length of 3-6 mm, and the pollen development period is the late mononuclear stage. ②Material sterilization: Use 7% (w / v) saturated calcium hypochlorite solution to disinfect the surface for 15 minutes, and rinse with sterile water 3 times. ③Collection and cultivation of microspores: put the sterile flower buds in a mortar, add a small amount of B5 washing medium (see the attached table for the formula), press the material lightly with the mortar to free the microspores from the anthers, use 45μm Filter with a pore size nylon mesh, collect the filtrate at 1000 rpm, centrifuge 3 times, 3 minutes each time, after the last centrifugation, suspend the microspores in the NLN medium (see the attached table for the recipe) sterilized by suction and filtration. Adjust the concentration of microspores to 1...

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Abstract

A method for culturing the regenerative plant of couliflower by free microspore culturing technique includes such steps as culturing until the microspore is generated, culturing at 25 deg.C and under 4000 LX for illumination (12-16 hr per day) until it becomes green, and culturing on non-hormone solid culture medium MS containing cane sugar (3%) until young seedling is grown up. It features that said solid culture medium contains also three auxins: kinin, 6-benzylpurine and naphthalene acetic acid. Its advantages are broad breeding resources and short breeding time.

Description

[0001] Field: [0002] The invention belongs to a method for cultivating vegetable breeding materials, in particular to a method for cultivating regenerated cauliflower plants using free microspore culture technology Background technique: [0003] At present, the free microspore culture technology in Brassica vegetable breeding is becoming more and more mature. Vegetables such as Chinese cabbage, Brassica napus, and cauliflower have been successfully cultured with free microspores, and have been used in breeding work. Cauliflower belongs to the Brassica genus vegetables, but it is difficult to obtain success by using the free microspore culture method of other Brassica vegetables. In particular, plant regeneration of cauliflower microspore embryos has always been an unresolved technical difficulty in the world. A certain number of spherical and heart-shaped embryos can be obtained by culturing cauliflower microspores, but the embryos at this time generally no longer continue t...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 孙德岭蔡荣期赵前程
Owner TIANJIN RES INST OF VEGETABLE
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