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Method using dominant single gene early blossoming material to carry out tobacco rapid pyramid breeding

A dominant single-gene, aggregated breeding technology, applied in the field of breeding, can solve the problems of high technical difficulty and difficult to popularize, and achieve the effect of enriching breeding resources

Inactive Publication Date: 2016-05-11
GUIZHOU TOBACCO SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the problems that the early flowering traits are rarely used in the existing conventional tobacco breeding process, there are risks in the breeding of the early flowering traits by the transgenic technology, and the technical difficulty is high and it is not easy to popularize, the present invention provides a method of using dominant single-gene early flowering materials for tobacco rapid Polybreeding method

Method used

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  • Method using dominant single gene early blossoming material to carry out tobacco rapid pyramid breeding

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Embodiment 1

[0048] In March 2012, the early-flowering mutant EF30 was used to carry out 4 consecutive generations of backcrossing with the mutant's source parent Yunyan 85, combined with the addition of southern propagation, and the genetic background was controlled by molecular markers. In March 2014, a homozygous genetic background was obtained. Yunyan 85 early flowering material (the obtained Yunyan 85 early flowering material can be reused as the homozygous early flowering core parent Q of the present invention).

[0049] The hybrid F 1 generation (60-70 days to obtain hybrids).

[0050] Planting Yunyan 85 early-flowering material / NC82's F 1 Dai, Yunyan 85 early flowering material / Bina No. 1 F 1 Generation, 60-70 days can be hybridized to obtain double-cross hybrids; plant double-cross hybrids, after 60-70 days, select more than 3 early-flowering single plants carrying target traits, use the flowers on the side branches to self-cross, and the main branches The flowers above are pol...

Embodiment 2

[0052] In December 2011, the early-flowering mutant EF30 was used to carry out 5 consecutive generations of backcrossing with the excellent line Guiyan 2 combined with the addition of southern propagation (recurrent parents can be sown in batches or topped for 1 year, and multiple backcrosses can be carried out). Molecular markers were used to control the genetic background, and the genetic background homozygous Guiyan No. 2 early flowering material was obtained in March 2014 (the obtained Guiyan No. 2 early flowering material can be repeated as the homozygous early flowering core parent Q of the present invention. use).

[0053] In July 2014, the high-quality variety K326, the disease-resistant variety NC729, and the high-yielding adaptable variety Nanjiang No. 3 were used as parents to be aggregated, and they were crossed with Guiyan No. 2 early flowering material (as the female parent) to obtain single-cross F 1 Generation (60-70 days to obtain hybrids), select two of them ...

Embodiment 3

[0056] In June 2012, the high-quality variety PVH06, the TMV-resistant variety Coker176, the adaptive variety Jiucaiping 2, and the mutant parent Yunyan 85 were used to cross with the early-flowering mutant EF30 to obtain single-cross F 1 Generation seeds, planted single-cross F 1 double cross F 1 (It only takes 60-70 days to harvest double cross F 1 ), planting in groups and planting double cross F in combination 1 After 60-70 days, each group selected more than 3 early-flowering individual plants, and used the flowers on the main branches as pollen receptors to cross with the early-flowering individual plants selected by another group to obtain four-cross F 1 seed, and the above selected double cross F 1 Flowers on lateral branches of a single plant are selfed. planting four cross F 1 Simultaneous planting of each double cross F 1 The selfed offspring of a single early flowering plant, if the selfed offspring does not segregate during the growth period, the double cros...

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Abstract

The invention discloses a method using a dominant single gene early blossoming material to carry out tobacco rapid pyramid breeding. The method comprises the following steps: (1) hybridizing a dominant single gene early blossoming material and a core parent, and then taking the core parent (P) as a recurrent parent to carry out continuous backcross until a homozygous early blossoming core parent with a homozygous genetic background is obtained; (2) preparing hybridization combinations from the homozygous early blossoming core parent and a plurality of parents with a normal blooming period to obtain multiple combined heterozygous early blossoming single cross F1; (3) integrating the excellent characteristics of the heterozygous early blossoming single cross F1 into the convergent cross species with a normal blooming period through convergent cross, wherein during the process of convergent cross, the flowers on the main branches are taken as the pollen acceptors to carry out hybridization, the flowers on the lateral branches carry out selfing, and the selfed seeds are subjected to early blossoming gene type identification so as to ensure that all early blossoming gene type of middle material are heterozygous; (4) taking the heterozygous cenospecies as the basic material to carry out breeding. The method can reduce 50% of time in each generation in pyramid breeding.

Description

technical field [0001] The invention relates to breeding technology, in particular to a method for rapid aggregation breeding of tobacco with dominant single gene early flowering materials. Background technique [0002] Tobacco (Nicotianatabacum L.) is an annual or limited perennial herb of the family Solanaceae, with a slightly lignified base. Inflorescences terminal, paniculate, many-flowered; capsules ovate or oblong, about as long as persistent calyx. It blooms and bears fruit in summer and autumn. Mainly distributed in South America, South Asia and China. [0003] Tobacco early flowering refers to the phenomenon that the tobacco plant buds and blooms earlier than the variety under normal local cultivation conditions. Due to the short vegetative growth time of tobacco plants with early flowering, there are problems such as low yield, and the tobacco varieties currently planted and promoted generally do not show early flowering. Chang Aixia et al. used early flowering...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 张吉顺张孝廉任学良王仁刚王轶
Owner GUIZHOU TOBACCO SCI RES INST
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