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Application of protein TabZIP60 in regulation of plant root system development

A plant root and protein technology, applied in applications, plant peptides, plant products, etc., can solve the problem of not much understanding of food crops related mechanisms, and achieve important application value and the effect of increasing total lateral root length.

Active Publication Date: 2018-11-23
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, although great progress has been made in the study of Arabidopsis root development in recent years, the relevant mechanisms of food crops such as wheat are not well understood.

Method used

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  • Application of protein TabZIP60 in regulation of plant root system development
  • Application of protein TabZIP60 in regulation of plant root system development
  • Application of protein TabZIP60 in regulation of plant root system development

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1, the construction of transgenic TabZIP60 wheat

[0057] 1. Preparation of Transgenic TabZIP60 Plants

[0058] (1) Acquisition of the TabZIP60 gene

[0059] 1. The total RNA of wheat variety KN199 was extracted, and its genomic cDNA was obtained by reverse transcription.

[0060] 2. Use the cDNA obtained in step 1 as a template, and use the following primers as primers to perform PCR amplification to construct the sequence required for overexpressing the TabZIP60 transgenic line wheat:

[0061] TabZIP60-OE-F: 5'- AAGCTT ATGGATTTTCCGGGAGGGAGCGGG-3' (the underlined sequence is the recognition site for Hind III digestion);

[0062] TabZIP60-OE-R: 5'- GAATTC TTACCAAGGGCCCGTCAGCGTCCTC-3' (the underlined sequence is the recognition site for EcoR I digestion).

[0063] Use the following primers as primers to perform PCR amplification to construct the required sequence for reducing the expression of the TabZIP60 transgenic line wheat:

[0064] TabZIP60-RNAi-...

Embodiment 2

[0106] Embodiment 2, root phenotype identification of TabZIP60 transgenic wheat

[0107] Tested wheat: three T2 generation TabZIP60 overexpressed wheat 60OE1, 60OE2 and 60OE3, three T2 generation TabZIP60 downexpressed wheat 60R1, 60R2 and 60R3, wild-type wheat KN199, and the empty control plant (NC).

[0108] Root phenotype identification was carried out under hydroponic conditions, and the specific steps were as follows:

[0109] The seeds of each tested wheat were treated overnight with 1% hydrogen peroxide to break dormancy, and then transplanted to seedling trays and cultivated for 7 days. When they grew to two leaves and one heart, the wheat seedlings with the same growth were selected and transplanted to high-nitrogen nutrient solution. (2mM N) and low-nitrogen nutrient solution (0.2mM N), and the formula of the culture solution is shown in Table 1 below. After 14 days of cultivation, the aboveground part and the root system were separated, and the dry weight of the ab...

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Abstract

The invention discloses application of a protein TabZIP60 in regulation of plant root system development. The invention provides the application of the TabZIP60 protein or related biological materialsthereof in regulation of plant root system development. The related biological materials are nucleic acid molecules capable of expressing the TabZIP60 protein, or expression cassettes, recombinant vectors, recombinant bacteria or transgenic cell lines, which contain the nucleic acid molecules. In the aspect of regulating plant root system development through the TabZIP60 protein, compared with wild type plants, the dry weight of root systems and / or the total lateral root length of transgenic plants in which TabZIP60 is over expressed is decreased obviously, and the dry weight of root systemsand / or the total lateral root length of transgenic plants in which TabZIP60 is less expressed is increased obviously. Therefore, the protein TabZIP60 can be utilized for regulating plant root system development. The application of the protein TabZIP60 in regulation of plant root system development has important application value to selective breeding of high-yield plant novel materials.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of the protein TabZIP60 in regulating the development of plant roots. Background technique [0002] The root is an important organ for plant growth and development, and its main functions are absorption, transportation, support, synthesis and storage. Plants absorb water, minerals and other nutrients from the soil through the root system, so the root system is extremely important to the growth and development of plants. There are significant differences between the root systems of monocotyledonous and dicotyledonous plants. Therefore, although great progress has been made in the study of Arabidopsis root development in recent years, the relevant mechanisms of food crops such as wheat are not well understood. [0003] Wheat is the main food crop, but in semi-arid dryland agricultural areas, the lack of water resources has become the main factor limiting the high yiel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/06
CPCC07K14/415C12N15/8261
Inventor 童依平杨军波何雪赵学强滕婉马文英
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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