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a sputum preservation solution

A technology for preserving liquid and sputum, applied in the biological field, can solve problems such as cumbersome and complicated operation processes, and achieve the effects of inhibiting RNase, improving accuracy, and less manual intervention

Active Publication Date: 2020-09-01
GUANGZHOU LBP MEDICINE SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If a sputum sample is to be subjected to different tests, such as thin-layer cell preparation and nucleic acid extraction, it needs to be processed with different processing solutions, and the operation process is cumbersome and complicated

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0031] A sputum preservation solution, consisting of the following components in the following contents: guanidine isothiocyanate 178g / L, 8-hydroxyquinoline 0.1M, sodium lauryl sarcosine 4 g / L, ethylene glycol Ammonium tetraacetic acid 5mM, potassium chloride 3 g / L, glycerin 5% v / v, isopropanol 10% v / v, proclin300 0.1% v / v, DEPC water balance.

Embodiment 2

[0033] A sputum preservation solution, consisting of the following components in the following contents: guanidine isothiocyanate 236g / L, 8-hydroxyquinoline 0.5M, sodium lauryl sarcosine 10g / L, ethylenediamine Tetraacetic acid 10mM, potassium chloride 10g / L, glycerol 10%v / v, isopropanol 15%v / v, proclin300 1%v / v, DEPC water balance.

Embodiment 3

[0035] A sputum preservation solution, consisting of the following components in the following contents: guanidine isothiocyanate 118g / L, 8-hydroxyquinoline 0.01M, sodium lauryl sarcosine 1g / L, ethylenediamine Tetraacetic acid 5mM, potassium chloride 1g / L, glycerol 5%, isopropanol 5%, proclin300 0.1%, DEPC water balance.

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Abstract

The invention provides a sputum preservation solution, which contains guanidine salt, metal chelating agent, surfactant and the like. The preservation solution of the present invention does not contain strong alkali components, which reduces the damage to nucleic acid, especially RNA. The preservation solution of the invention can keep the sputum sample stable, quickly liquefy the sputum, inhibit RNase, obtain high-purity RNA, and improve detection accuracy. The liquefied sputum sample was directly extracted for RNA, and the yield and purity were better than those of the comparison method. The samples preserved in the preservation solution of the present invention can be used in various methods such as magnetic bead method, column extraction method, cracking method, etc., without interference. The liquefied sputum samples can be directly used for cytological detection, and no effect on cell density or cell staining was seen.

Description

technical field [0001] The invention relates to the field of biotechnology, more specifically, to a sputum preservation solution. Background technique [0002] The new coronavirus 2019-nCoV is a newly discovered coronavirus, the main route of transmission is through respiratory droplets and contact transmission, and there is a possibility of aerosol transmission when exposed to high-concentration aerosols for a long time in a relatively closed environment; The population is generally susceptible. [0003] Hong Kong University Pan Liewen et al [Pan Y, Zhang D, Yang P, et al. Viral load of SARS-CoV-2in clinical samples[J]. The Lancet Infectious Diseases, 2020.] in throat swabs, sputum, urine, In comparison with stool samples, sputum samples generally had higher viral loads than throat swab samples. No 2019-nCoV RNA was detected in either urine or stool samples. An article published on the medRxiv preprint platform by Zhongnan Hospital of Wuhan University and Jinyintan Hospi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/10
CPCC12N15/10
Inventor 陈绍宇何瑰黄颖李楚明刘慧米小娟
Owner GUANGZHOU LBP MEDICINE SCI & TECH
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