Solid-state culture method for improving content of nerve-protecting component-containing hericium erinaceus extract A of hericium erinaceus

A technology of solid-state culture and solid-state culture medium, which is applied in the fields of mushroom cultivation, botany equipment and methods, cultivation, etc. It can solve the problems of difficult heterogeneous conditions for solid-state culture, waste environmental pollution, and high demand for sterility, and achieve production costs. Inexpensive, complex operation, simple operation effect

Inactive Publication Date: 2020-06-05
SYI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, due to the high aseptic requirements of liquid culture, various chemical agents need to be added depending on the culture situation, and the waste after culture is likely to cause environmental pollution, there is still room for improvement in terms of manpower, equipment, management, and cost of the culture process. ; and although solid-state culture has the advantages of simple operation, no space occupation, low production cost, and easy waste disposal without pollution concerns, it is difficult to uniformly control the heterogeneous conditions of solid-state culture and have many parameters, so it is difficult to optimize, and the yield is not as good as that of liquid culture. high, so there are still restrictions on the use of

Method used

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  • Solid-state culture method for improving content of nerve-protecting component-containing hericium erinaceus extract A of hericium erinaceus
  • Solid-state culture method for improving content of nerve-protecting component-containing hericium erinaceus extract A of hericium erinaceus
  • Solid-state culture method for improving content of nerve-protecting component-containing hericium erinaceus extract A of hericium erinaceus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, single factor test

[0041] In order to explore the solid-state culture method of optimizing Hericine A (Erinacine A) (hereinafter referred to as Erinacine A) output, at first, first carry out the single factor experiment of multiple carbon sources, multiple nitrogen sources, and multiple nutritional supplements , to screen each group for those who have a greater impact on the output of Hericin A content, each classification group and the results are as follows:

[0042] (1) Carbon source test

[0043] Add 3% glucose (glucose), fructose (fructose), sucrose (sucrose), maltose (maltose), or lactose (lactose) respectively as the test group, carry out solid-state culture, and detect each variable, the solid-state culture product monkey The content of cephalin A, among which 3.85 mg / g in the sucrose group and 3.2 mg / g in the glucose group, yields the most excellent yields.

[0044] (2) Nitrogen source test

[0045] Add 1% peptone (peptone), meat peptone (mea...

Embodiment 2

[0048] Embodiment 2. Response surface method (RSM) experiment

[0049] 2. 1-Partial Factorial Design (Two-level Factorial Design)

[0050] According to the results of the single factor test, sucrose, glucose, casein peptone, polyvalent peptone, citric acid and oleic acid were selected as the test factors (independent variables), and 16 groups were designed by the partial factor test method. Test medium (table 1) carries out solid-state culture, and wherein, " 1 ", "-1 " shown in code (coded) in table 1 represents that addition is high or low level respectively; The yield results of element A were statistically analyzed with Design-Expert software, and the results indicated the degree of influence of each factor on the yield of erinacein A (Table 2). Based on this, the key factor of carbon source, sucrose, and the key factor of nitrogen source, casein peptone, were selected to make as the main factor in subsequent experiments.

[0051] Table 1. Partial factorial experimental ...

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Abstract

The invention relates to the field of mushroom culture, in particular to a solid-state culture method for increasing the content of a nerve-protecting component-containing hericium erinaceus extract Aof hericium erinaceus. The invention provides a solid culture medium and a culture method thereof which are used for culturing the nerve-protecting component-containing hericium erinaceus extract A of the hericium erinaceus, adding the hericium erinaceus mycelia to the solid culture medium, and culturing the hericium erinaceus mycelia at 20-30 DEG C, wherein the solid culture medium comprises cereals and a nutrient solution, and the nutrient solution comprises sucrose, casein peptone and a selectively added nutrient additive. The pH value of the nutrient solution is 5.0-6.5, the weight percentage of the sucrose is 0.75-2.2%, and the weight percentage of the casein peptone is 3-4%. The present invention also relates to a neuroprotective composition comprising a culture product of the solid-state culture method and the hericium erinaceus extract A.

Description

technical field [0001] The invention relates to the field of cultivation of mushrooms, in particular to a solid-state cultivation method for enhancing the nerve-protecting component erinacein A contained in Hericium erinaceus. Background technique [0002] Hericium erinaceus (Hericium erinaceus), in taxonomy, belongs to the Kingdom Fungi, Phylum Fungi, Phylomycetes, Polyporacea, Dentaceae, Hericium; also known as Hericium, Hericium, Hedgehog, for its Named after the shape, it is distributed in the upright trunks of broad-leaved forests in temperate regions of the world. Both edible and medicinal fungi, mycelium and fruiting bodies can be used as medicine. The fruiting bodies themselves are rich in polysaccharides, fatty acids, ergosterol, cerium compounds, erinaceous ketones , double linseed oil phospholipid ethylene amine, amino acids, trace elements and other active ingredients, which are recorded in traditional Chinese medicine can help digestion, benefit the five interna...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20
CPCA01G18/00A01G18/20
Inventor 连秀屘陈家章张筱君林永浩
Owner SYI BIOTECH CO LTD
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