Broad spectrum antibody spray for SARS-CoV-2 and SARS-CoV

A coronavirus, a new type of technology, applied in the direction of antiviral agents, antibodies, antiviral immunoglobulin, etc., can solve the problem of lack of specific, safe and effective products, and achieve the effect of protecting susceptible people, low cost, and rapid preparation

Inactive Publication Date: 2020-06-05
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the prevention and treatment of coronavirus, household or medical disinfectants approved by various countries can effectively kill the ne...

Method used

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  • Broad spectrum antibody spray for SARS-CoV-2 and SARS-CoV

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] This example provides a composite IgY antibody against novel coronavirus pneumonia and atypical pneumonia, and the specific preparation method is as follows:

[0037] 1. Prepare the composite antigen of SARS-CoV-2 and SARS-CoV

[0038] (1) Construction of SARS-CoV-2 and SARS-CoV composite S protein

[0039] Two viruses, SARS-CoV-2 and SARS-CoV eukaryotic expression vector pcDNA6B-FLAG-S were constructed, respectively transfected into HEK-293 cells, and stable expression strains were obtained through resistance screening, and the cells were lysed after expanding culture, RT-PCR and Western blot identification, Flag adsorption column affinity chromatography purification of S protein, enzyme digestion to remove FLAG tag.

[0040] (2) Construction of SARS-CoV-2 and SARS-CoV S protein receptor binding domain epitopes: application of bioinformatics to predict protein secondary structures and HLA epitopes of the two virus subtypes; overlapping PCR to construct RBD fusion gene...

Embodiment 2

[0068] This embodiment provides a broad-spectrum antibody spray for novel coronavirus and SARS virus, the specific preparation steps are as follows:

[0069] Specific chicken egg yolk antibody (SARS-CoV-2 antibody ≥ 1:32 and SARS-CoV antibody ≥ 1:32); excipients dosage (mass percentage content): mint essence 0.1%, sucralose 0.08%, glycine 0.2% Wait.

Embodiment 3

[0071] This embodiment provides the usage method of the broad-spectrum antibody spray for novel coronavirus and SARS virus that embodiment 2 provides:

[0072] The broad-spectrum antibody spray for the new coronavirus and SARS virus is a specific compound IgY antibody spray, which can specifically bind to the S structural protein of SARS-CoV-2 and SARS-CoV virus, and is mainly used for neutralization, Kill viruses in the oropharynx, nasal cavity and skin.

[0073] 1. Dosage form and specifications: aqueous solution spray, 18ml / bottle;

[0074] 2. Composition and traits: This strain uses the complex S antigen or epitope of SARS-CoV-2 and SARS-CoV to immunize laying hens, collect eggs, separate and purify IgY immunoglobulin, and sterilize and virus After inactivation, it is prepared by adding an appropriate amount of stabilizer. Colorless, clear, opalescent liquid.

[0075] 3. Main components and content: composite IgY antibody against new coronary pneumonia and atypical pneu...

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Abstract

The invention discloses a composite IgY antibody for resisting COVID-19 and SARS. The antibody is prepared by the following method: constructing a recombinant antigen of SARS-CoV-2 and SARS-CoV; preparing an immune preparation with the recombinant antigen to immunize egg-producing birds, and obtaining the specific composite IgY antibody from the immunized eggs. A spray prepared by the antibody canbe used for broad-spectrum killing of SARS-CoV-2 and SARS-CoV, and is suitable for spraying on nasal cavity, oral cavity, skin and other parts. The antibody preparation has a specific virus neutralizing effect, can effectively control infection sources, protect susceptible people, disinfect and protect first-line medical staff and prevent spread of SARS-CoV-2.

Description

technical field [0001] The invention relates to specific IgY and a preparation method thereof, in particular to a broad-spectrum antibody spray for novel coronavirus and SARS virus. Background technique [0002] The acute coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a public health emergency of international concern. SARS-CoV-2 has infected a large number of people, is highly transmissible, and has a high fatality rate. It is urgent to seek safe and efficient specific drug treatment. As a biological agent that can specifically recognize antigens and exert humoral immune function, therapeutic antibody has the characteristics of strong function and high targeting. [0003] SARS-CoV-2 is a single-stranded positive-strand RNA virus. The full genome sequence shows that it has a structural similarity with SARS-CoV of up to 80%, and a receptor binding domain (RBD) homology of 73%. The sister virus of CoV, bu...

Claims

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Application Information

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IPC IPC(8): A61K39/42A61P31/14C07K16/02C07K16/10
CPCA61K9/0014A61K9/0043A61K9/006A61K39/42A61P31/14C07K16/02C07K16/10C07K2317/11
Inventor 李燕沈鑫卫韡包丽荣晏彩霞张程李佳李明远
Owner SICHUAN UNIV
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