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Detection of short-chain fatty acids in mouse gut-brain axis-related tissue samples based on gc-ms

A technology for medium and short-chain fatty acids and detection methods, applied in the field of analytical chemistry, can solve the problems of complicated operation steps, low recovery results, low sensitivity, etc., and achieve the effects of good repeatability and high recovery

Active Publication Date: 2022-07-26
SHANDONG SHANWEI IMMUNOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The inventors found that there is no report on the quantitative detection method of short-chain fatty acids in brain tissue and colon tissue. At present, the detection methods for short-chain fatty acids in colon contents and feces mainly include liquid chromatography, gas chromatography and gas chromatography. Chromatography and mass spectrometry, etc., but due to its cumbersome operation steps, high time cost, low recovery results, low sensitivity, poor stability and other problems have not been widely used

Method used

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  • Detection of short-chain fatty acids in mouse gut-brain axis-related tissue samples based on gc-ms
  • Detection of short-chain fatty acids in mouse gut-brain axis-related tissue samples based on gc-ms
  • Detection of short-chain fatty acids in mouse gut-brain axis-related tissue samples based on gc-ms

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Embodiment 1

[0033] (1) Sample pretreatment

[0034] Brain tissue: Accurately weigh 0.5 g of brain tissue, add 2 mL of pre-cooled ultrapure water, use a tissue grinder to grind uniformly at 4 °C, vortex vigorously for 1 min, extract by ultrasonic for 15 min, and centrifuge at 4 °C and 10,000 rpm for 10 min. Take the supernatant;

[0035] Colon tissue: The pretreatment method was the same as that of brain tissue.

[0036] Colon content: Accurately weigh 0.5g of colon content, add 2.5mL of pre-cooled ultrapure water, grind uniformly at 4°C using a tissue grinder, vortex vigorously for 1min, extract by ultrasonic for 15min, 4°C, 10000rpm Centrifuge for 10 min, take the supernatant and dilute it 2 times with pre-cooled ultrapure water;

[0037] Stool: Pretreatment method is the same as for colonic contents.

[0038] (2) Sample pretreatment

[0039]Pipette 100 μL of tissue extract with a pipette, put it in a clean 2 mL capped glass tube, add 10 μL of 2 mM 2-ethylbutyric acid internal standa...

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Abstract

The invention discloses a method for detecting short-chain fatty acids in mouse gut-brain axis related tissues, involving brain tissue, colon tissue, colon contents and feces, and comprising the following steps: detecting brain tissue, colon tissue, colon contents and feces. Pretreatment of feces to obtain brain tissue, colon tissue, colon contents and fecal extract; adding internal standard to brain tissue, colon tissue, colon contents and fecal extract, and adding pentafluorobromine for derivatization reaction ; After the derivatized solution is extracted, the acetic acid, propionic acid and butyric acid in the extract are detected by GC-MS. The invention establishes a gas chromatography-mass spectrometry rapid determination method for detecting three kinds of short-chain fatty acids in brain tissue, colon tissue, colon content and feces through a derivatization method. Pre-process and derivatize different biological samples, and add internal standard for correction to achieve accurate quantification.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a GS-MS-based detection method for short-chain fatty acids in mouse gut-brain axis-related tissue samples, mainly involving brain tissue, colon tissue, colon contents and feces . Background technique [0002] The disclosure of information in this Background section is only for enhancement of understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. [0003] Short chain fatty acids are the main metabolites produced after dietary fiber is digested and decomposed in the large intestine, mainly including acetic acid, propionic acid and butyric acid. The highest concentrations of SCFAs are found in the cecum and proximal colon, where they are the main energy source for colon cells. In additio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/045G01N2030/067G01N2030/062
Inventor 宋月曲新艳王泉博
Owner SHANDONG SHANWEI IMMUNOTECH CO LTD
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