Alpha-galactosyl ceramide fat emulsion and preparation method thereof

A technology based on ceramide and galactosyl, which is applied in the field of medicine, can solve the problems of inconvenient clinical reference, poor water solubility of antigen, and failure of NKT cells, and achieve the effects of avoiding NKT failure, simple preparation method, and easy popularization and application

Pending Publication Date: 2020-06-09
CHENGDU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the water solubility of these glycolipid antigens is extremely poor, which brings inconvenience to clinical reference
In addition, even after dissolving these glycolipid antigens by adding latent solvents, in vivo experiments have found that multiple intravenous injections of these water-soluble antigen molecules can cause the failure of NKT cells

Method used

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  • Alpha-galactosyl ceramide fat emulsion and preparation method thereof
  • Alpha-galactosyl ceramide fat emulsion and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1. Preparation of α-galactosylceramide fat emulsion of the present invention

[0036] Prepare 10mL of α-galactosylceramide fat emulsion: Weigh 1g of soybean oil into a beaker, heat to 60°C, add 0.12g of lecithin and 0.01g of α-galactosylceramide into the soybean oil, and stir at 5000r / min , to get the oil phase. Add 0.225 g of glycerin into the water for injection, and heat to 60° C. to dissolve to obtain the aqueous phase. Put the water phase and the oil at the same time into the mixer, stir at 10000r / min for 5min, stir into colostrum, homogenize the colostrum with high-pressure milk (800bar, 8 times), adjust the pH value to 6.5 with sodium hydroxide solution, and pack , 115 ° C autoclave for 30 minutes, you can.

Embodiment 2

[0037] Example 2, Preparation of α-galactosylceramide fat emulsion of the present invention

[0038] Prepare 10mL of α-galactosylceramide fat emulsion: Weigh 2g of soybean oil into a beaker, heat to 60°C, add 0.12g of lecithin and 0.03g of α-galactosylceramide into the soybean oil, and stir at a high speed of 5000r / min , to get the oil phase. Add 0.225 g of glycerin into the water for injection, and heat to 60° C. to dissolve to obtain the aqueous phase. Put the water phase and the oil at the same time into the mixer, stir at 10000r / min for 5min, stir into colostrum, homogenize the colostrum with high-pressure milk (800bar, 8 times), adjust the pH value to 6.5 with sodium hydroxide solution, and pack , 115 ° C autoclave for 30 minutes, you can.

Embodiment 3

[0039] Example 3, Preparation of α-galactosylceramide fat emulsion of the present invention

[0040] Prepare 10mL of α-galactosylceramide fat emulsion: Weigh 3g of soybean oil into a beaker, heat to 60°C, add 0.12g of lecithin and 0.03g of α-galactosylceramide into the soybean oil, and stir at a high speed of 5000r / min , to get the oil phase. Add 0.225 g of glycerin into the water for injection, and heat to 60° C. to dissolve to obtain the aqueous phase. Put the water phase and the oil at the same time into the mixer, stir at 10000r / min for 5min, stir into colostrum, homogenize the colostrum with high-pressure milk (800bar, 8 times), adjust the pH value to 6.5 with sodium hydroxide solution, and pack , 115 ° C autoclave for 30 minutes, you can.

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Abstract

The invention discloses an alpha-galactosyl ceramide fat emulsion. Every 1 liter of the alpha-galactosyl ceramide fat emulsion is prepared from the following raw materials by weight: 0.01-20g of alpha-galactosyl ceramide, 50-500g of injection oil, 1-100g of an emulsifier, 5-100g of an isoosmotic adjusting agent and the balance of injection water. According to the alpha-galactosyl ceramide fat emulsion disclosed by the invention, the alpha-galactosyl ceramide is emulsified into the emulsion by using the emulsifier, not only is stability high, but also solubilization of the antigens can be achieved, and in addition, the problem of NKT (natural killer T) failure caused by multiple times of medicine administration can be avoided. The alpha-galactosyl ceramide fat emulsion disclosed by the invention is simple in preparation method and easy to popularize and apply.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to an alpha-galactosylceramide fat emulsion and a preparation method thereof. Background technique [0002] NKT (natural killer T) cells are a group of special T cell subsets that have both T cell receptor TCR and NK cell receptor on the cell surface. NKT cells have immunoregulatory and cytotoxic effects. After NKT cells are stimulated, they can secrete a large amount of IL-4, IFN-γ, GM-CSF, IL-13 and other cytokines and chemokines to play an immune regulatory role. At the same time, NKT cells are also one of the bridges connecting innate immunity and acquired immunity. Therefore, NKT cells show great promise for the treatment of major diseases such as tumors. [0003] One of the realization methods of NKT cell therapy is to extract the patient's own blood for in vitro culture, and the white blood cells collected in the culture contain monocytes. Special substances are used to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61K9/19A61K9/107A61K47/24A61P35/00A61P31/12A61P37/02
CPCA61K39/0005A61K9/19A61K9/107A61K9/0019A61K47/24A61P35/00A61P31/12A61P37/02
Inventor 张全许小红叶静鲍莎朱昱锦
Owner CHENGDU MEDICAL COLLEGE
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