Superoxide dismutase solid preparation and preparation method thereof
A dismutase solid, superoxide technology, applied in the biological field, can solve problems to be studied and so on
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[0052] In another aspect of the present invention, the present invention proposes a method for preparing the aforementioned solid preparation of superoxide dismutase. According to an embodiment of the present invention, see figure 1 , the preparation method comprises:
[0053] S100 Extraction processing
[0054] In this step, the raw material is subjected to extraction treatment so as to obtain an extract containing superoxide dismutase. Thereby, in order to extract superoxide dismutase from raw material.
[0055] According to an embodiment of the present invention, the method for obtaining the extract includes: subjecting edible plants or fermented liquid containing microorganisms to cell disruption treatment to make a slurry, so as to obtain the extract. Thus, to facilitate the release of superoxide dismutase.
[0056] According to an embodiment of the present invention, the microorganism is a genetically engineered bacterium, and the genetically engineered bacterium con...
Embodiment 1
[0074] In this embodiment, prepare SOD solid powder according to the following method:
[0075] (1) select the fruit raw material of the fresh non-rotten prickly pear for use;
[0076] (2) Clean up;
[0077] (3) Mixing Rosa roxburghii fruit with ice water, crushing the cells, and making a slurry at 4-10°C;
[0078] (4) filter with a 60-mesh filter screen, separate the peel, pulp residue, seeds and juice, and obtain the juice;
[0079] (5) Centrifuge the juice, wherein the centrifugal speed is 3000 rpm, the time is 10 min, and the supernatant is collected;
[0080] (6) The supernatant is subjected to double-channel ultrafiltration separation treatment, wherein the first separation adopts a tubular membrane with a pore size of 100 kD, and the second separation adopts a coiled membrane with a pore size of 10 kD to collect the retentate;
[0081] (7) The retentate was subjected to high-pressure kinase treatment, wherein the pressure was 550 MPa, the time was 5 min, and the temp...
Embodiment 2
[0086] In this embodiment, prepare SOD solid powder according to the following method:
[0087] (1) select the fruit raw material of the fresh non-rotten prickly pear for use;
[0088] (2) Clean up;
[0089] (3) Mixing Rosa roxburghii fruit with ice water, crushing the cells, and making a slurry at 4-10°C;
[0090] (4) filter with a 60-mesh filter screen, separate the peel, pulp residue, seeds and juice, and obtain the juice;
[0091] (5) Centrifuge the juice, wherein the centrifugal speed is 2000 rpm, the time is 15 min, and the supernatant is collected;
[0092] (6) The supernatant is subjected to double-channel ultrafiltration separation treatment, wherein the first separation adopts a tubular membrane with a pore size of 80 kD, and the second separation adopts a coiled membrane with a pore size of 20 kD to collect the retentate;
[0093] (7) The retentate was subjected to high-pressure kinase treatment, wherein the pressure was 450 MPa, the time was 4 min, and the tempe...
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