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Method for improving gonad development efficiency of female parents of paracanthobrama guichenoti bleeker

A parental and female technology is applied in the field of improving the developmental efficiency of the female parental gonad of the bream, and achieves the effect of significant economic benefits

Inactive Publication Date: 2020-06-23
SHANGHAI FISHERIES RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report about a method of the present invention to improve the gonad development efficiency of the female parent of the bream-like bream

Method used

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  • Method for improving gonad development efficiency of female parents of paracanthobrama guichenoti bleeker
  • Method for improving gonad development efficiency of female parents of paracanthobrama guichenoti bleeker
  • Method for improving gonad development efficiency of female parents of paracanthobrama guichenoti bleeker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Reproductive Peak Experiment

[0036] Through continuous follow-up sampling of the female bream, and dissecting and counting the ovarian index, it can be found that the oviposition mode of the bream is continuous, and the spawning cycle is long (see Table 2), and the ovary can still be found until July In mature individuals, the ovary develops continuously, and there are oocytes in various phases in the ovary at the same time, which are matured step by step and replenished continuously.

[0037] Table 2. Seasonal changes in gonad development of female parents of G. spp.

[0038]

[0039]

[0040] Nevertheless, through statistical analysis, it can be seen that the spawning period of large-scale individuals is obviously longer than that of small individuals. Although the ovaries of female individuals weighing about 76g can develop (the smallest sexually mature individual in this experiment is 76.35g), but 60-80g Size parents have low fecundity and a short...

Embodiment 2

[0041] Embodiment 2 nutritional fortification experiment

[0042]Dissected 42 female parental samples of the bream that were sampled in the waters of Dianshan Lake from April to May, and analyzed their feeding habits, among which 37 had food in their intestines. The food types in the intestinal tract include algae, plants and seeds, cladocera, crustaceans, shellfish, adult insects of the order Diptera, insects of the order Hymenoptera, insects of the order Coleoptera, annelids, phylum oligochaete, fish eggs, small fish remains, etc. , In addition, there are sand particles in the intestinal tract of some individuals. Diptera insects (mainly adults and larvae of mosquitoes and flies), herbaceous plants, and algae have the highest occurrence rate in the diet; followed by Annelida oligochaeta, Hymenoptera insects (ants, bees), crustaceans. Cladocera, small fish, coleopteran insects, shellfish, and fish eggs had a lower occurrence rate than the above groups. It can be seen that ...

Embodiment 3

[0049] Embodiment 3 effect experiment

[0050] 1 Experimental method

[0051] Carry out artificial reproduction comparison between the parents of bream-like bream after nutritional fortification of A3 formula in Example 2 and wild parents, wherein the conditions for oxytocin are: oxytocic agent: luteinizing hormone releasing hormone combined with dioconone, one-time treatment of mature broodstock For intrapleural injection, the drug and dosage for female fish are: dioconone 5 μg / kg + luteinizing hormone releasing hormone (LHRH-A2) 7 μg / kg, and the injection volume for male fish is halved. Put into circular plastic vat (diameter is 2m, water depth is 70cm) after induced labor, stimulate with micro-flow water, and observe the estrus situation of male and female fish.

[0052] After ten hours of effect time, the broodstock was caught. At this time, when the abdomen of the female fish was lightly pinched, yellow eggs flowed out. The collected eggs were collected in a sterilized w...

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Abstract

The invention relates to a method for improving the gonad development efficiency of female parents of paracanthobrama guichenoti bleeker, to be specific, a most suitable size of female parents for artificial breeding is determined by track sampling of the law of gonad development of wild parents, and researching induced spawning effects of different induced spawning doses on different sizes of paracanthobrama guichenoti bleeker parents; through trophic analysis of the wild parents, most suitable nutrient requirements during the breeding season of the paracanthobrama guichenoti bleeker are determined and optimized special feed is prepared, and nutrient enrichment is performed on the paracanthobrama guichenoti bleeker parents before artificial breeding; and a best parent breeding way is verified by comparing artificial propagation performance of the wild parents with that of the artificial nutrition enriched parents. Results are shown as follows: the large-size parents are selected for artificial breeding (150-180g), and artificial breeding duration is 4-5 months; nutrition enrichment is performed on the female parents before artificial breeding for no less than 4 weeks; and vitaminE is added to the feed by 200mg / kg. The method has the advantages that the propagation performance of the paracanthobrama guichenoti bleeker, and the fertilization rate and the hatching rate of artificial reproduction are significantly improved.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a method for improving the gonad development efficiency of the female parent of the bream bream. Background technique [0002] Paracanthobrama guichenoti Bleeker (Paracanthobrama guichenoti Bleeker), which belongs to Cyprinidae, Cyprinidae, Subfamily Paracanthobrama, and the genus Paracanthobrama guichenoti, is commonly known as stone crucian carp. It is mainly distributed in the middle and lower reaches of the Yangtze River and lakes in China. Although it is small in size, its meat is tender and delicious. It has a population distribution in the affiliated lakes of the lower reaches of the Yangtze River, such as Chaohu Lake, Taihu Lake, Dianshan Lake, etc., and has certain economic value. With the pollution of water environment and human overfishing, the living space of the bream is seriously threatened, and the number of bream populations in the waters has decreased sharply...

Claims

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Application Information

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IPC IPC(8): A01K61/10A23K10/16A23K10/18A23K10/20A23K10/22A23K10/30A23K10/37A23K20/142A23K20/158A23K20/174A23K20/20A61D7/00A61K38/09A61K31/454A61P15/08
CPCA61D7/00A61K31/454A61K38/09A23K10/16A23K10/18A23K10/20A23K10/22A23K10/30A23K10/37A23K20/142A23K20/158A23K20/174A23K20/20A01K61/10A61P15/08A61K2300/00Y02P60/87Y02A40/81
Inventor 杨茜刘智俊施顺昌徐琴英李建忠
Owner SHANGHAI FISHERIES RES INST
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