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Preparation method and application for CdTe labeled antibodies and method for detecting polybrominated biphenyl

A technology of polybrominated biphenyls and labeled antibodies, applied in chemical instruments and methods, biological testing, measuring devices, etc., can solve the problems of high environmental conditions and personnel requirements, high analysis costs, time-consuming operations, etc., and achieve easy popularization and promotion, analysis capacity Large, sensitive effects

Pending Publication Date: 2020-06-23
INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Therefore, the technical problem to be solved by the present invention lies in the defects of the prior art that the detection method of polybrominated biphenyls has complex steps, time-consuming operation, high analysis cost, and high requirements on environmental conditions and personnel, thereby providing a preparation method, application and application of a CdTe-labeled antibody. Methods for the detection of polybrominated biphenyls

Method used

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  • Preparation method and application for CdTe labeled antibodies and method for detecting polybrominated biphenyl
  • Preparation method and application for CdTe labeled antibodies and method for detecting polybrominated biphenyl
  • Preparation method and application for CdTe labeled antibodies and method for detecting polybrominated biphenyl

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Embodiment 1

[0048] This embodiment prepares a kind of preparation method of CdTe labeled antibody, comprises the following steps:

[0049] (1) Add 0.1276g Te powder and 0.1123g NaHB4 into a small glass bottle containing 4mL, quickly seal the seal with a ziplock bag, communicate with the outside world with an injection needle, stir with strong magnetic force and react in an ice-water bath for 4-6 hours , when the color of the solution gradually turns into light pink, and white crystals appear in the reaction bottle, it is the prepared NaHTe solution;

[0050] (2) Add 0.059g of CdCl to the round bottom flask 2 2.5H 2 O and 50μL thioglycolic acid in 180mL of high-purity water, through high-purity nitrogen for 30min, with 1.00mol L -1 The NaOH solution was adjusted to a pH value of about 12.0, passed through high-purity nitrogen for 30 minutes, and then 50 μL of the NaHTe solution prepared above was added under the protection of nitrogen, and the color of the solution immediately became lig...

Embodiment 2

[0056] This embodiment prepares a kind of preparation method of CdTe labeled antibody, comprises the following steps:

[0057] (1) Add 0.1276g Te powder and 0.1123g NaHB4 to a small glass bottle containing 4mL, quickly seal the seal with a ziplock bag, communicate with the outside world with an injection needle, stir with strong magnetic force and react in an ice-water bath for 5 hours. The color of the solution gradually turns into light pink, and when white crystals appear in the reaction bottle, it is the prepared NaHTe solution;

[0058] (2) Add 0.02g of CdCl to the round bottom flask 2 2.5H 2 O and 25μL thioglycolic acid in 180mL of high-purity water, through high-purity nitrogen for 30min, with 1.00mol L -1 The NaOH solution was adjusted to a pH value of about 12.0, and high-purity nitrogen gas was passed for 30 minutes, and then 20 μL of the NaHTe solution prepared above was added under nitrogen protection, and the color of the solution immediately became light yellow...

Embodiment 3

[0064] This embodiment prepares a kind of preparation method of CdTe labeled antibody, comprises the following steps:

[0065] (1) Add 0.1276g Te powder and 0.1123g NaHB4 to a small glass bottle containing 4mL, quickly seal the seal with a ziplock bag, communicate with the outside world with an injection needle, stir with strong magnetic force and react in an ice-water bath for 5 hours. The color of the solution gradually turns into light pink, and when white crystals appear in the reaction bottle, it is the prepared NaHTe solution;

[0066] (2) Add 0.04g of CdCl to the round bottom flask 2 2.5H 2 O and 35μL thioglycolic acid in 180mL of high-purity water, through high-purity nitrogen for 30min, with 1.00mol L -1 The NaOH solution was adjusted to a pH value of about 12.0, passed high-purity nitrogen for 30 minutes, and then added 40 μL of the above-prepared NaHTe solution under nitrogen protection, and the color of the solution immediately became light yellow to obtain a CdT...

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Abstract

The invention discloses CdTe labeled antibodies and a method for detecting polybrominated biphenyl by using the CdTe labeled antibodies. The method comprises the following steps: preparing the CdTe labeled antibodies, carrying out a competitive reaction on a to-be-detected sample and the CdTe labeled antibodies, carrying out washing to remove redundant free labeled antibodies, and placing obtainedCdTe labeled antibodies onto a fluorescence microplate reader for reading. When the quantity of the CdTe labeled antibodies is fixed, the more the content of the polybrominated biphenyl in the to-be-detected sample is, the less the quantity of the CdTe labeled antibodies combined with a solid-phase antigen is, while the lower the fluorescence value is, the higher the inhibition rate is; otherwise, the higher the fluorescence value is, the lower the inhibition rate is; and the content of the polybrominated biphenyl in the to-be-detected sample can be calculated according to a pre-obtained polybrominated biphenyl standard curve and the inhibition rate of the to-be-detected sample. The detection method disclosed by the invention has the advantages of strong specificity, high sensitivity, convenience, rapidness, large analysis capacity, safety, reliability, no need of expensive instruments and complex pretreatment processes, low professional technical requirements on analysts, and easiness in popularization and promotion.

Description

technical field [0001] The invention relates to the field of detection of polybrominated biphenyls, in particular to a preparation method and application of a CdTe-labeled antibody and a method for detecting polybrominated biphenyls. Background technique [0002] Polybrominated biphenyls (PBBs) are a kind of brominated flame retardants, which are widely used in building materials, textiles, chemicals and other industries due to their excellent flame retardant efficiency and thermal stability. PBBs and their metabolites can cause acute or chronic toxicity to human brain, liver, kidney and other organs as well as nervous system, endocrine system, reproductive system, etc. Degradation, high lipophilicity, bioaccumulation and amplification effect. In view of their toxicity, the application of PBBs has been closely watched and controlled. In order to protect the environment and human ecological health, PBBs organic pollutants have been listed as prohibited by many laws, regulat...

Claims

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Application Information

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IPC IPC(8): C07K16/44C07K16/06G01N33/53
CPCC07K16/065C07K16/44G01N33/5308
Inventor 张金艳魏益华熊艳赖艳邱素艳刘廷强陈庆隆严寒昌晓宇庄惠生
Owner INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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