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A cryoprotectant for human testicular tissue suspension and preparation method thereof

A technology of cryoprotectant and testis, which is applied in the field of cryoprotectant and its preparation, which can solve problems such as difficult to find and poor effect of cryoresuscitation, and achieve the effect of increasing quantity, increasing clinical utilization rate, and reducing osmotic pressure difference

Inactive Publication Date: 2021-04-20
THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a cryoprotectant for human testicular tissue suspension and a preparation method thereof. The cryoprotectant is specially designed for the testicular tissue suspension, which can improve the cryopreservation effect of testicular sperm and effectively solve the problem of It has very important clinical application value to solve the problems of potential safety hazards, poor cryopreservation effect and difficulty in finding motile spermatozoa.

Method used

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  • A cryoprotectant for human testicular tissue suspension and preparation method thereof
  • A cryoprotectant for human testicular tissue suspension and preparation method thereof
  • A cryoprotectant for human testicular tissue suspension and preparation method thereof

Examples

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Embodiment 1

[0024] A human testicular tissue suspension cryoprotectant, each 100mL includes the following raw materials by weight: 0.35g of sodium chloride, 0.03g of potassium chloride, 0.035g of calcium chloride, 0.008g of magnesium chloride, 0.004g of potassium dihydrogen phosphate, and sodium bicarbonate 0.2g, HEPES 0.3g, caffeine 0.035g, sodium pyruvate 0.0017g, pentoxifylline 0.102g, glycine 0.5g, glucose 0.08g and sucrose 1g; also includes sodium lactate 130μL and glycerin 10mL.

[0025] The preparation method of above-mentioned human testis tissue suspension cryoprotectant, comprises the following steps:

[0026] (1) Weigh successively sodium chloride, potassium chloride, calcium chloride, magnesium chloride, potassium dihydrogen phosphate, sodium bicarbonate, HEPES, caffeine, sodium pyruvate, pentoxifylline, glycine, glucose and sucrose, add After the ultrapure water is dissolved, a mixed solution is obtained;

[0027] (2) Add sodium lactate and glycerin to the mixed solution obt...

Embodiment 2

[0029] A human testis tissue suspension cryoprotectant, every 100mL human testis tissue suspension cryoprotectant comprises the following weight raw materials: sodium chloride 0.584g, potassium chloride 0.04g, calcium chloride 0.04g, magnesium chloride 0.01g, diphosphate Potassium hydrogen 0.005g, sodium bicarbonate 0.26g, HEPES 0.477g, caffeine 0.048g, sodium pyruvate 0.0028g, pentoxifylline 0.208g, glycine 1g, glucose 0.109g and sucrose 1.712g; also includes sodium lactate 188μL and glycerin 30mL.

[0030] The preparation method of above-mentioned human testis tissue suspension cryoprotectant, comprises the following steps:

[0031] (1) Weigh successively sodium chloride, potassium chloride, calcium chloride, magnesium chloride, potassium dihydrogen phosphate, sodium bicarbonate, HEPES, caffeine, sodium pyruvate, pentoxifylline, glycine, glucose and sucrose, add After the ultrapure water is dissolved, a mixed solution is obtained;

[0032] (2) Add sodium lactate and glycer...

Embodiment 3

[0034] A human testicular tissue suspension cryoprotectant, each 100mL includes the following raw materials by weight: 0.65g of sodium chloride, 0.045g of potassium chloride, 0.047g of calcium chloride, 0.0151g of magnesium chloride, 0.007g of potassium dihydrogen phosphate, and sodium bicarbonate 0.32g, HEPES 0.55g, caffeine 0.058g, sodium pyruvate 0.0035g, pentoxifylline 0.555g, glycine 1.3g, glucose 0.209g and sucrose 2.5g; also includes sodium lactate 280μL and glycerol 65mL.

[0035]The preparation method of above-mentioned human testis tissue suspension cryoprotectant, comprises the following steps:

[0036] (1) Weigh successively sodium chloride, potassium chloride, calcium chloride, magnesium chloride, potassium dihydrogen phosphate, sodium bicarbonate, HEPES, caffeine, sodium pyruvate, pentoxifylline, glycine, glucose and sucrose, add After the ultrapure water is dissolved, a mixed solution is obtained;

[0037] (2) Add sodium lactate and glycerin to the mixed soluti...

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Abstract

The invention discloses a human testis tissue suspension cryoprotectant and a preparation method thereof. The human testis tissue suspension cryoprotectant comprises the following raw materials: sodium chloride, potassium chloride, calcium chloride, magnesium chloride, potassium dihydrogen phosphate, Sodium bicarbonate, HEPES, caffeine, sodium pyruvate, pentoxifylline, glycine, dextrose, and sucrose; human testicular tissue suspension cryoprotectants also include sodium lactate and glycerin. The present invention also includes a preparation method of the cryoprotectant of the human testis tissue suspension and a freezing method using the cryoprotectant of the human testis tissue suspension. The invention specially designs a cryoprotectant for the testicular tissue suspension, which can improve the effect of cryopreservation of testicular sperm, effectively solve the problems of potential safety hazards, poor cryopreservation effect, and difficulty in finding active testicular sperm, and has very important clinical application value .

Description

technical field [0001] The invention relates to the technical field of cryoprotectant and its preparation, in particular to a human testicular tissue suspension cryoprotectant and a preparation method thereof. Background technique [0002] With the development of social industrialization, environmental pollution is becoming more and more serious, and the proportion of infertility is increasing. The incidence rate among couples of childbearing age is as high as 9.3%, and among them, the male factor accounts for about 30-50%. Among the male factors, The proportion of patients with azoospermia is as high as 10-20%. In 1992, the emergence of intracytoplasmic sperm injection (ICSI) technology realized the wish of male patients with oligospermia to have offspring. In 1993, Schoysman used testicular sperm to perform ICSI and achieved success. Clinical application brings good news. Clinically, on the basis of excluding genetic diseases, patients with azoospermia usually undergo te...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02C12N5/076
CPCA01N1/0226C12N5/061
Inventor 李福平鲜泱刘博蒋敏柳莎莎刘晓赵文瑞
Owner THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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