Recombinant HIV (human immunodeficiency virus) defective virus and preparation method thereof

Abstract

The invention provides a recombinant HIV (human immunodeficiency virus) defective virus and a preparation method thereof. According to the recombinant HIV defective virus, a virus of an identical subtype of HIV, which is carried by an infected person, is recombined into a defective virus, most parts of an env region are deleted, an intron with three groups of four +1 section double-chain miRNA (micro ribonucleic acid) is inserted, and the defective virus is completely identical to an HIV gene sequence expect env, can be replicated by self, and can be a defective virus with infection through anenv protein generated by HIV. When the defective virus is subjected to reverse transcription into a cell genome, becomes a previrus and is subjected to transcription to generate a sub-virus, former mRNA (messenger ribonucleic acid) generating env is sheared to remove the intron, then a great deal of double-chain miRNA can be generated, RNAi (ribonucleic acid interfere) can be generated, HIV sub-viruses, mRNA of env and new invading viruses can be killed, and thus an effect of treating Aids can be achieved.

Description

technical field [0001] The invention relates to the field of clinical medicine, in particular to a recombinant HIV deficient virus and a preparation method thereof. Background technique [0002] Since 1981 when the US CDC first reported the HIV virus, AIDS has spread at an alarming rate in the past 38 years and has become a serious global public health problem. According to the World Health Organization, there are currently about 36.7 million HIV-infected people in the world, with about 2 million new infections every year and more than 35 million deaths. According to a joint assessment by the Chinese Center for Disease Control and Prevention, UNAIDS, and the World Health Organization, by the end of 2018, there were estimated to be about 1.25 million surviving HIV-infected people in China. As of the end of September 2018, 850,000 surviving infections and 262,000 deaths were reported across the country. It is estimated that there are about 80,000 new infections every year. ...

AI Technical Summary

Problems solved by technology

Moreover, the carriers used are all disposable, requiring multiple injections for treatment, which is prone to immune reactions, and it is impossible to cure HIV infection in this direction

[0012] To sum up, there is currently no scientific research on the complete cure of HIV virus infection in the world, let alone a clinical treatment method to cure HIV virus infection

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