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Potato microtuber breeding method

A test-tube potato and potato technology, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of long breeding cycle, high cost, low efficiency, etc., achieve low cost, simple preparation steps, and convenient operation procedures easy-to-learn effect

Pending Publication Date: 2020-07-07
甘肃康勤薯业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the above problems, the present invention provides a method for breeding potato tubers, which solves technical problems such as long breeding cycle, high cost, and low efficiency, and is efficient, high-quality, labor-saving, easy to operate and easy to manage.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A kind of breeding method of potato test tube tuber, comprises the following steps:

[0026] (1) Prepare virus-free seedlings: cut off the terminal buds of the virus-free test-tube seedlings, and cut the stems with 3-4 axillary buds;

[0027] (2) Strong seedling cultivation: the stem section is transferred to a culture bottle equipped with a liquid medium; the liquid strong seedling medium is used to cultivate the stem section into a seedling. The formula of the liquid medium is: macroelement 2xPBM, microelement 1xPBM, sucrose 19g / L, inositol 100mg / L, niacin 0.50mg / L, glycine 20mg / L. pH 6.0. Use 12% hydrochloric acid solution to adjust the pH.

[0028] The macroelements are: KNO 3 1890mg / L, NH 4 NO 3 1540mg / L, KH 2 PO 4 162mg / L, MgSO 4· 7H 2 O350mg / L, CaCl 2· 2H 2 O 450mg / L;

[0029] Trace elements are: KI 0.82mg / L, H 3 BO 4 5.8mg / L, MnSO 4· 4H 2 O 22.3mg / L, ZnSO 4· 7H 2 O7.5mg / L, Na 2 MoO 4· 2H 2 O 0.023mg / L, CuSO 4· 5H 2 O 0.028mg / L, CoCl 2...

Embodiment 2

[0039] Other content is as content in embodiment 1, wherein:

[0040] (1) Under sterile conditions, cut off the terminal buds of the virus-free test-tube seedlings, cut off the stems with 3-4 axillary buds and transfer them to culture bottles filled with liquid medium. The culture medium adopts the developed liquid culture medium, 2xPBM macroelements, 1xPBM microelements, sucrose 19g / L, organic matter is the same as MS), pH value 5.9, use filter paper as a bridge, sterilize at 121℃ for 23min, fill each bottle with about 20mL culture medium 2500lx light intensity, 16h / d light, and 24°C temperature. In about 3 weeks, strong seedlings with 5-7 nodes can be grown.

[0041] After cutting the terminal buds of the virus-free potato test-tube plantlets cultivated by solid strong seedlings, they are directly added to the liquid medium for the production of test tube tubers; the clipped terminal buds can also be inserted into the solid medium for strong seedling cultivation, used as Fo...

Embodiment 3

[0047] Other content is as content in embodiment 1, and the breeding method of potato test-tube tuber, concrete steps are as follows:

[0048] (1) Prepare virus-free seedlings: cut off the terminal buds of the virus-free test-tube seedlings, and cut the stems with 3-4 axillary buds;

[0049] (2) Strong seedling cultivation: the stem section is transferred to a culture bottle equipped with a liquid medium; the liquid strong seedling medium is used to cultivate the stem section into a seedling. The formula of the liquid medium is: macroelement 2xPBM, microelement 1xPBM, sucrose 19g / L, organic matter same as MS, pH value 5.8. Use 12% hydrochloric acid solution to adjust the pH.

[0050] Use filter paper as a bridge, stir all the raw materials evenly, place them in a high-temperature sterilizer and sterilize them at 121°C for 23 minutes, fill each bottle with about 20mL of culture medium, and insert 5 stem segments.

[0051] The culture conditions are light intensity 2000-3000lx...

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Abstract

The invention belongs to the field of agricultural cultivation and production, and in particular relates to a potato microtuber breeding method. The potato microtuber breeding method comprises the steps of: preparing virus-free seedlings: cutting off terminal buds of virus-free test-tube seedlings, and obtaining a stem segment with 3-4 axillary buds by cutting; culturing strong seedlings: transferring the stem segment into a culture bottle with a liquid culture medium, so that the stem segment is cultured into seedlings; performing induction culturing: after forming seedlings, pouring out liquid culture solution residue in the step (2), adding a liquid-induced culture medium into a container, and performing induced tuberization; and harvesting after performing induction culture for 50 d. The potato microtuber breeding method in the invention solves the technical problems of long breeding period, high cost, low efficiency and the like, and has the advantages of being high-efficiency, high in quality, labour-saving, easy to operate and convenient to manage.

Description

technical field [0001] The invention belongs to the field of agricultural cultivation and production, and in particular relates to a method for breeding test tube tubers of potatoes. Background technique [0002] Potato is a rare high-yielding crop in the world. It has excellent characteristics such as comprehensive nutrition, wide adaptability, strong stress resistance, high and stable yield, long industrial chain, and dual use as raw materials for grain, vegetable and feed industries. It is the fourth largest food crop in the world. An important factor affecting the low yield of potato is the virus degeneration of potato. Potatoes have an average yield reduction of 30%-80% after virus infection. It is meaningful to vigorously strengthen the production and seed breeding of virus-free potatoes, improve the supply capacity of virus-free potatoes, accelerate the popularization and application of virus-free potatoes, and steadily increase the per unit yield and total output of...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 康潇霄
Owner 甘肃康勤薯业有限公司
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