Rhizopus stolonifer fl-3 and its application in extracting Poria cocos polysaccharide

A technology of rhizopus stoloniferus and FL-3, which is applied in the field of microbial fermentation technology in the extraction of Poria cocos polysaccharides, can solve the problems of large differences in polysaccharide yields, achieve the effect of increasing extraction yields and promoting dissociation

Active Publication Date: 2022-03-18
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Different extraction processes have their own advantages and disadvantages, and the yield of polysaccharides also varies greatly

Method used

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  • Rhizopus stolonifer fl-3 and its application in extracting Poria cocos polysaccharide
  • Rhizopus stolonifer fl-3 and its application in extracting Poria cocos polysaccharide
  • Rhizopus stolonifer fl-3 and its application in extracting Poria cocos polysaccharide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Screening of strain FL-3

[0021] (1) Add about 10 g of Poria cocos powder that has been crushed through a 60-mesh sieve into a 250-mL Erlenmeyer flask, then add 20 mL of sterile water to moisten it, and incubate at a constant temperature of 28°C for 4 days. Dilute the enriched culture covered with mold with sterile water 1 × 10 6 After doubling, spread it on the PDA plate medium, culture at 28°C for 2 days, pick out mold colonies with different colors and shapes, transfer them to fresh PDA medium, place them in 28°C for 3 days, and obtain 6 purely cultured strains, respectively Numbered FL-1~FL-6. Add 5 mL of sterile water to the fresh plate cultures of the 6 strains, stir with an inoculation loop to suspend the spores, transfer the spore liquid to a sterile test tube, and adjust the spores with sterile water. The concentration is 1×10 8 individual / mL.

[0022] (2) 250-mL triangular flask is sterilized by dry heat at 160°C for 2 hours, add 10 g of Poria cocos pow...

Embodiment 2

[0035] Rhizopus stoloniferus FL-3 is applied to the extraction of polysaccharides, and can be operated according to the following steps:

[0036] (1) Preparation of spore liquid of Rhizopus stoloniferus FL-3: cryopreserved Rhizopus stoloniferus FL-3 (freeze-dried tube or plate colony) was inoculated on fresh PDA plate medium, cultured at 28°C for 3 days, and added 5 mL Put sterile water in a petri dish, stir with an inoculation loop to suspend the spores, transfer the spore liquid to a sterile test tube, and adjust the spore concentration to 1×10 with sterile water. 8 individual / mL. Described PDA plate medium composition and preparation method are the same as embodiment 1.

[0037] (2) 500-mL Erlenmeyer flask was sterilized by dry heat at 160°C for 2 hours, added 20 g of Poria cocos powder crushed through a 60-mesh sieve, added 60 mL of sterile water, and then inoculated with 2 mL of Rhizopus stoloniferus FL- 3 spore liquid, stir well. The mouth of the triangular flask was ...

Embodiment 3

[0042] Rhizopus stoloniferus FL-3 is applied to the extraction of polysaccharides, and can be operated according to the following steps:

[0043] (1) Preparation of spore liquid of Rhizopus stoloniferus FL-3: cryopreserved Rhizopus stoloniferus FL-3 (freeze-dried tube or plate colony) was inoculated on fresh PDA plate medium, cultured at 28°C for 3 days, and added 5 mL Put sterile water in a petri dish, stir with an inoculation loop to suspend the spores, transfer the spore liquid to a sterile test tube, and adjust the spore concentration to 2×10 with sterile water 8 individual / mL. Described PDA plate medium composition and preparation method are the same as embodiment 1.

[0044] (2) 2-L Erlenmeyer flask was sterilized by dry heat at 160°C for 2 hours, added 50 g of Poria cocos powder crushed through a 60-mesh sieve, added 150 mL of sterile water, and then inoculated with 5 mL of the spore liquid prepared in step (1) of FL-3 , stir well. The mouth of the triangular flask w...

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Abstract

The invention discloses a strain of Rhizopus stolonifer FL-3 and its application in extracting Poria cocos polysaccharide. The application method is: inoculating the spores of Rhizopus stolonifer FL-3 into Poria powder containing sterile water, Ferment at 28-30°C for 2-3 days, and the fermented product is ultrasonically extracted and then concentrated to obtain a concentrate; then the concentrate is precipitated and vacuum-dried to obtain pachymane. In advance of the supersonic water of pachyrhin, the fermentation pretreatment of Rhizopus stolonifer FL‑3 was added. Rhizopus stolonifer FL‑3 is an excellent strain isolated from the microbial enrichment culture of Poria cocos and obtained through screening. It grows moderately in poria cocos powder and produces polysaccharide hydrolase, which hydrolyzes the insoluble polysaccharide structure of Poria cocos and promotes the binding state. For the dissociation of soluble polysaccharides, compared with the conventional method without fermentation pretreatment, the extraction yield of pachyrhiza can be increased by more than 30%.

Description

(1) Technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to the application of a microbial fermentation technology in the extraction of pachyrhiza. (2) Background technology [0002] The traditional Chinese medicine Poria (Poria), also known as Poria rabbit and Songbaiyu, is the dried sclerotia of the fungus Poria cocos Wolf in the family Polyporaceae. Poria cocos is a traditional Chinese herbal medicine for both medicine and food. It is recorded in the 2015 Chinese Pharmacopoeia. Its nature and flavor are sweet, light and flat; Cure mainly edema, oliguria, dizziness and palpitation due to phlegm retention, insufficiency of the spleen, lack of food, loose stool and diarrhea, restlessness, palpitation and insomnia. Poria cocos is one of the components of the traditional Chinese medicine "Four Monarchs and Eight Treasures". It has a wide range of uses and a large dosage. There are hundreds of Chinese patent med...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N1/14C12P19/04C08B37/00C12R1/845
CPCC12P19/04C08B37/0003C12R2001/845C12N1/145
Inventor梅建凤郑素晶吴霞应国清易喻
OwnerZHEJIANG UNIV OF TECH