Aspergillus aculeatus NM-11-6 and application thereof in lemon essential oil extraction
A technology of Aspergillus aculeatus and lemon essential oil, applied in the field of bioengineering, can solve the problems of insignificant, low cellulose content, limited hydrolysis ability of plant tissue, etc., and achieves the effects of improving the extraction yield and improving the extraction yield.
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Embodiment 1
[0022] The screening of embodiment 1 fermentation strain
[0023] In order to screen a strain that is suitable for lemon peel fermentation to improve the yield of essential oil extraction, the present invention uses the following method for screening:
[0024] (1) In a 250-mL Erlenmeyer flask sterilized by dry heat at 160°C for 2 hours, add 40 g of fresh lemon peel cut into small pieces with a side length of 2–4 mm, tie the mouth with 8 layers of gauze, and incubate at a constant temperature of 28°C for 72 hours. The enriched cultures covered with mold were diluted 1×10 with sterile saline -6 , 1×10 -7 , 1×10 -8 After doubling, draw 0.1mL of the diluted solution and spread it on the potato dextrose agar plate medium (PDA), culture at a constant temperature of 28°C for 48 hours, pick mold colonies with different colors and shapes and transfer them to fresh PDA plate medium, and place them at 28°C. Cultivate at constant temperature for 60 hours to obtain 14 purely cultured st...
Embodiment 2
[0035] Secondary separation and purification of embodiment 2 lemon peel fermentation strain NM-11
[0036] The strain NM-11 was subjected to secondary single spore isolation and purification, and the excellent strains used for fermentation and pretreatment of lemon peel to improve the extraction rate of essential oil were screened. The specific method was as follows:
[0037] (1) Preparation of spore liquid: after strain NM-11 was activated and cultivated on PDA plate medium at 28°C for 48 hours, 5 mL of sterile normal saline was added, stirred with an inoculation loop to suspend the spores, and then transferred to a triangle containing 45 mL of sterile normal saline. In the bottle (with 20–30 glass beads), shake at room temperature for 15 minutes. The spore suspension was filtered to remove mycelium (triangular funnel pad with 2 layers of lens-cleaning paper). Dilute 1×10 with sterile saline -6 , 1×10 -7 , 1×10 -8 After doubling, pipette 0.1 mL of different dilutions of t...
Embodiment 3
[0042] Classification and Identification of Example 3 Bacterial Strain NM-11-6
[0043] Strain NM-11-6 is inoculated on PDA plate medium, such as single-point inoculation, after 24 hours of cultivation at 28 ° C, gray-white mycelium grows, and the colony is radial. After 48 hours, dark brown conidia are produced in the center of the colony, and the edge is still gray-white Mycelia; such as streak inoculation, after culturing at 28°C for 24 hours, off-white mycelium grows on both sides of the inoculation line, and after 48 hours, a large number of conidia are produced on the surface of the colony; the conidiophores are erect or slightly curved, and the conidia heads are spherical Or subspherical, single-layered stalk; conidia approximately spherical or elliptic, with radial spines on the surface, 4–5 μm in diameter. The colony photo of strain NM-11-6 after streak inoculation on PDA plate medium and cultured at 28°C for 48 hours is shown in figure 1 .
[0044] The measured rDN...
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