53 results about "Rhizopus nigricans" patented technology

The invention discloses a method of a two-step united multi-strain fermented feed, and belongs to the technical field of feeds. The fermented feed disclosed by the invention is obtained through two steps of aerobic fermentation and anaerobic fermentation by using maize flour, palm dregs, soybean meal, bran and middling as raw materials and by using rhizopus nigricans, aspergillus oryzae, bacillus subtilis, lactobacillus plantarum and beer yeast as fermenting strains. The feed fermented through the method disclosed by the invention has the advantages that the protein content is as high as 30-34% which is increased by 45-62% than that of raw materials, the content of alpha-amylase is as high as 90-110U / g, the enzyme activity of acidic protein is as high as 110-130U / g, and the feed is rich in protein, rich in probiotics and high in enzymic activity. The method disclosed by the invention has the characteristics of high maneuverability, being suitable for large-scale industrial production and the like, and has favorable market prospects.

The invention discloses composite bacteria for removing oil from kitchen waste and a preparation method for the composite bacteria. The preparation method comprises the following steps of: performing high-density culture on strains of trichoderma viride, bacillus subtilis, black rhizopus, photosynthetic bacteria, candida lipolytica, bacillus licheniformis, nitrosomonas europaea and nitrobacter winogradskyi respectively to obtain microbial dry hypopus powder; and mixing 15 to 20 weight parts of trichoderma viride, 10 to 15 weight parts of bacillus subtilis, 25 to 40 weight parts of black rhizopus, 5 to 10 weight parts of photosynthetic bacteria, 5 to 10 weight parts of candida lipolytica, 10 to 15 weight parts of bacillus licheniformis, 5 to 15 weight parts of nitrosomonas europaea and 5 to 15 weight parts of nitrobacter winogradskyi to obtain the composite bacteria for removing the oil from the kitchen waste. The composite bacteria can quickly decompose lipid ingredients in the kitchen waste when used for treating the kitchen waste; and compared with the prior art, the method has the advantages that: the production investment and the production cost are low, and the method is convenient to use.

The invention discloses food waste deodorization composite bacteria and a preparation method thereof, and the preparation method comprises the following steps: respectively performing high-density culture on strains of trichoderma viride, bacillus subtilis, bread mold, photosynthetic bacteria, saccharomyces cerevisiae, bacillus licheniformis, nitrosomonas europaea and nitrobacterwinogradskyi, performing dehydration and drying on various types of monoxenie obtained by high-density culture to prepare hypopus microbial dry powder, and then mixing according to the weight ratio of 10-20 parts of the trichoderma viride, 10-15 parts of the bacillus subtilis, 20-30 parts of the bread mold, 5-10 parts of the photosynthetic bacteria, 10-20 parts of the saccharomyces cerevisiae, 10-15 parts of the bacillus licheniformis, 5-15 parts of the nitrosomonas europaea and the 5-15 parts of the nitrobacterwinogradskyi to prepare the food waste deodorization composite bacteria. The food waste deodorization composite bacteria are used for deodorization of waste; and compared with the prior art, the method has the advantages that the production investment is low, the production cost is low, the use is convenient and the deodorization effect is good.

The invention, relating to the field of pharmaceutical synthesis, particularly relates to a preparation method of hydrocortisone, comprising the following steps: using an intermediate 17 alfa-hydroxy-4-pregnene-3,11,20- triketone (II) in a Rhizopus nigricans method as raw material, successively carrying out ketal reaction, reduction reaction, hydrolysis reaction, iodine adding reaction, and displacement reaction to obtain hydrocortisone acetate (VII), and finally carrying out sodium hydroxide hydrolysis reaction to obtain the hydrocortisone (I). The invention has the advantages of easy obtainment of raw material, common auxiliary materials, and no need of using toxic, highly toxic, and carcinogenic reagents. The method is more efficient than the process in the prior art, and the products has good quality and yield.

The invention relates to a compound bacterial agent for efficiently converting pig excrements, and a preparation method thereof comprises the following steps of: (1) respectively carrying out activation and expanding culture on bacillus subtilis powder and Lactobacillus plantarum powder to obtain two bacterial suspensions, then inoculating the two bacterial suspensions to a bacterial fermentation medium in a volume ratio of 1:1 for compound fermentation co-culture, and subsequently, filtering fermentation liquor, adding a protective agent to the obtained filtrate and carrying out spray drying to prepare compound bacterial powder; (2) respectively carrying out activation and expanding culture on mucor racemosus powder, aspergillus niger powder, Aspergillus oryzae powder, Rhizopus nigricans powder and Candida utilis powder to obtain five bacterial suspensions, then inoculating the five bacterial suspensions to a fungus fermentation medium in a volume ratio of 1:1:1:1:1 for ventilation compound co-culture, and subsequently filtering fermentation liquor, adding a protective agent to the obtained filtrate and carrying out spray drying to prepare compound fungus powder; and (3) metering the compound bacterial powder and the compound fungus powder in a weight ratio of 1:1 and evenly mixing.

The invention relates to a Kefir source Lactobacillus paracasei subsp. tolerans strain with antimicrobial properties and its bacteriostatic composition. The strain FX-6-1 provided by the invention is preserved in China Center for Type Culture Collection on Feb. 23, 2014 with a preservation number of CCTCC M 2014043. The bacteriostatic composition generated by fermentation of the strain can inhibit the growth of Escherichia coli, Staphylococcus aureus and other bacterial pathogenic microorganisms, and Aspergillus flavus, Rhizopus nigricans and other moulds, and has a wide antibacterial spectrum. The bacteriostatic composition generated by fermentation of the strain provided by the invention shows stable properties on heat treatment and enzymolysis treatment, can be used as a preservative in feed and food, and has the potential of development into a medical composition.

The present invention provides a compound Xiaoqu, which comprises mother koji and yeast, wherein the microorganisms in the mother koji comprise Aspergillus niger, Rhizopus nigricans, Rhizopus oryzae and Aspergillus oryzae, the yeast comprises ester-producing yeast and Saccharomyces cerevisiae, a weight ratio of the Aspergillus niger to the Rhizopus nigricans to the Rhizopus oryzae to the Aspergillus oryzae is 14:2:3:1, a weight volume ratio of the mother koji to the ester-producing yeast is 100 g:20 ml, a weight volume ratio of the mother koji to the Saccharomyces cerevisiae is 100 g:10 ml, and the yeast numbers in the ester-producing yeast and the Saccharomyces cerevisiae are not less than 1*10<8> / mL. According to the present invention, the wind producing capacity of the compound Xiaoqu is improved; the ester production ability is storing; and through the gas chromatography analysis, the number and the type of the produced flavor substances are increased compared to the ordinary Xiaoqu.

The invention relates to a preparation method and application of total nutrient microorganism biocontrol organic fertilizer. The preparation method of the total nutrient microorganism biocontrol organic fertilizer comprises the following steps: firstly, carrying out plate cultivation on trichoderma pseudokoningii TRIPI and rhizopus nigricans RN-9; secondly, carrying out PDB liquid culture on TRIPI and RN-9 hypha blocks; thirdly, carrying out fermentation culture on a liquid culture solution, so that fermentation liquor is obtained; fourthly, inoculating the fermentation liquor to farmyard manure raw materials, uniformly mixing, stockpiling and fermenting, so that a fermented material is obtained; fifthly, adding other ingredients into the fermented material, and uniformly mixing, so that the total nutrient microorganism biocontrol organic fertilizer is obtained. The prepared total nutrient microorganism biocontrol organic fertilizer has high inhibition ratio on pathogenic bacteria and high relative control efficiency on fusarium wilt of cucumber; compared with biocontrol organic fertilizer prepared by adopting single strain fermentation liquor, content of pathogenic bacteria in cucumber rhizosphere soil, cucumber root system and cucumber stalk is low, and cucumber blade protective enzyme is quick in response, high in activity and long in duration of time.

The invention relates to rhizopus nigricans polysaccharide which has the activity of inhibiting growth of a cell BGC-823 and an application thereof. Rhizopus nigricans RN-9 with the rhizopus nigricans polysaccharide which has the activity of inhibiting growth of a cell BGC-823 of gastric cancer is preserved in China General Microbiological Culture Collection Center on 5th, November, 2013 with the preservation number of CGMCC NO.8436. The invention further provides the application of RN-9 in preparing rhizopus nigricans polysaccharide which has the activity of inhibiting growth of a cell BGC-823 of gastric cancer. The RN-9 provided by the invention has the function of generating rhizopus nigricans polysaccharide which has the activity of inhibiting growth of the cell BGC-823 of gastric cancer.

The invention relates to an application of rhizopus nigricans exopolysaccharides in preparation of a medicine for treating or preventing gastrointestinal tumors. The method for preparing the rhizopus nigricans exopolysaccharides comprises the following steps: (1) activating and performing fermental cultivation on rhizopus nigricans with a strain collection number of CGMCC NO.8436, and preparing fermentation liquor; (2) separating and purifying the fermentation liquor, and preparing a crude extracting solution; and (3) discoloring the crude extracting solution by resin columns, oscillating to remove proteins through a Seveage reagent, performing column chromatography purification on Sephadex G-50 sephadex, and preparing the rhizopus nigricans exopolysaccharides. An animal tumor model is established by utilizing the rhizopus nigricans exopolysaccharides, the tumor activity of an entity anti-alimentary system of the rhizopus nigricans exopolysaccharides is explored, the animal tumor experiment anti-tumor result proves that the rhizopus nigricans exopolysaccharides can obviously inhibit growth of tumor blocks of stomach cancer tumor mice and colon cancer tumor mice, and when the rhizopus nigricans exopolysaccharides are combined with modern chemotherapy drug cyclophosphamide, the anti-tumor curative effect is obviously higher than the anti-tumor effect of a single cyclophosphamide drug.

The invention provides an application of G6PDH gene for improving steroid C11 alpha-hydroxylation ability of rhizopus nigricans, a method for constructing glucose-6-phosphate dehydrogenase genetically engineered bacteria and a strain screened out after construction. G6PDH is cloned into rhizopus nigricans through a molecular biological method to transform the strain, and the G6PDH catalyzes NADPH regeneration in the transformed rhizopus nigricans cells to provide coenzyme necessary for a catalytic reaction of a cytochrome P450 enzyme system so as to improve the steroid C11 alpha-hydroxylation ability of the rhizopus nigricans. The application provided by the invention has the beneficial effects that the genetically engineered bacteria created by the method provided by the invention are used for converting Wolfowitz oxide C11 alpha-hydroxylation to produce mold oxide; compared with a starting strain, the strain grows faster and is high in raw material utilization rate and high in conversion rate and conversion efficiency, so that the strain has far-reaching theoretical significance and high application value in development and application process of steroid medicine.

The invention provides a method for improving the fermentation yield of natamycin, and relates to the method for improving the fermentation yield of natamycin. The fermentation yield of the natamycin is improved; meanwhile, the renninum fermentation waste thalli are reasonably used; waste materials are changed into valuable materials. The method comprises the following steps of filtering rice rhizopus nigricans thalli to remove excessive water; preparing suspension; adding lyticase into the suspension for wall breaking enzymolysis to obtain enzymolysis liquid; fermenting 0 to 48h under the existence of natamycin; adding the enzymolysis liquid into the natamycin culture medium; obtaining natamycin after the fermentation. The method has the advantages that the rice rhizopus nigricans fermentation waste thalli for producing chymosin are added into the natamycin fermentation culture medium, so that the yield for producing the strain natamycin can be obviously improved; meanwhile, the problem that the rice rhizopus nigricans fermentation waste thalli are difficult to treat is solved; double benefits are achieved.

The invention relates to mixed distiller's yeast for glutinous rice wine and a preparation method of the mixed distiller's yeast, and belongs to the technical field of distiller's yeast processing. The mixed distiller's yeast for the glutinous rice wine, provided by the invention, comprises the following components in percentage by weight: 68-72 percent of rhizopus oryzae bacterial liquid, 18-22 percent of saccharomyces cerevisiae bacterial liquid, 4.8-5.2 percent of rhizopus chinentis bacterial liquid and 4.8-5.2 percent of rhizopus nigricans bacterial liquid; the bacteria concentration of the rhizopus oryzae bacterial liquid is 1*10<3>-1*10<4> cfu / mL; the bacteria concentration of the saccharomyces cerevisiae bacterial liquid is 1*10<6>-1*10<7> cfu / mL; the bacteria concentration of the rhizopus chinentis bacterial liquid is 1*10<2>-1*10<3> cfu / mL; the bacteria concentration of the rhizopus nigricans bacterial liquid is 1*10<2>-1*10<3> cfu / mL. The mixed distiller's yeast is obtained according to the preparation method provided by the invention. The mixed distiller's yeast provided by the invention is easy to store and can be directly used for the production of the glutinous rice wine, and the produced glutinous rice wine is stable in flavor and high in safety performance.

The invention discloses a microbial preparation for rapid treatment of soil with heavy metal pollution and a production method thereof. The microbial preparation comprises the following components in parts by weight: 25-40 parts of an Aspergillus niger agent, 25-40 parts of a Rhizopus nigricans agent, 10-15 parts of a Saccharomyces cerevisiae agent, 5-10 parts of a filamentous cyanobacteria agent, and 15-20 parts of a Listeria monocytogenes agent; the Aspergillus niger agent is preserved in GSH protective solution; the Saccharomyces cerevisiae agent is preserved in distilled water; the filamentous cyanobacteria agent is preserved in a glycerin solution whose mass percent is 30%; the Listeria monocytogenes agent is preserved in a glycerin solution whose mass percent is 10%. The invention also discloses a purification, cultivation, preservation and production method of the microbial preparation. The microbial preparation has the advantages of multiple bacterial strains which effective complemented, high efficiency, good economical efficiency, and wide application range.

The invention provides a brewing process for preparing nutritious vinegar by mixed fermentation through composite strains. The raw materials used in the brewing process include grain materials, distiller's yeast, and composite strains prepared koji, wherein the composite strains included in the composite strains prepared koji consist of more than one of Rhizopus nigricans, saccharomycetes and acetic bacteria. The grain materials are cleaned, soaked in water and stirred. The grain materialsare fully cooked after soaking, cooled to room temperature by spraying cold water, and mixed with koji andplaced in a fermentation tank, pre-fermentation is performed after evenly mixing, the composite strains prepared koji is added into the fermentation tank after the completion of the pre-fermentationand evenly mixed, and the nutritious vinegar is obtained by sealed fermentation for more than 18 days. The brewing process for preparing the nutritious vinegar by mixed fermentation through the composite strains adopts the composite strains to prepare the nutritious vinegar through mixed fermentation. The prepared nutritious vinegar is dark brown, bright in colour and lustre, has no obvious precipitation, is fragrant and strong in aroma, sour but not astringent in taste, fragrant and slightly sweet, has no foreign smell and is nutritious.

The invention discloses a composite bacterial liquid and a preparation method thereof. The preparation method comprises 1, nutrient solution preparation: taking 20-30 parts by weight of malt meal and 60-80 parts by weight of starch, carrying out starch pasting, adding malt meal into the paste, carrying out saccharification and carrying out vortex separation to obtain a culture solution, 2, inoculation: inoculating the culture solution with mixed culture comprising equal weights of rhizopus nigricans, aspergillus niger, monascus, geotrichum candidum and rice wine yeast, 3, fermentation, and 4, filtration: removing solids in the fermentation broth by a filer to obtain a composite bacteria liquid. Through combination with corresponding traditional Chinese medicines, the composite bacterial liquid can improve traditional Chinese medicine effects.

The invention relates to a rhizopus nigricans hypha liposome direct transformation method. The method includes the steps of transformation of rhizopus nigricans hypha liposome and subculture. The steps of the transformation of the rhizopus nigricans hypha liposome comprise firstly mixing the tender rhizopus nigricans hypha and mannitol water solutions, grinding and obtaining bacterium suspension for use; secondly uniformly mixing liposome 2000 and plasmid pEGFP-C1, standing 15-60 minutes at the temperature of -5-5 DEG C, then transferring the mixture to the bacterium suspension, uniformly mixing, and standing 15-60 minutes at the temperature of -5-5 DEG C; and thirdly coating the mixing liquid obtained from the second step on a potato dextrose agar (PDA) plate, and cultivating the mixing liquid for 1-5 days at the temperature of 25-30 DEG C. The steps of the subculture comprise picking transformant which is provided with fluorescent though detection of a fluorescent microscope in the third step, inoculating the transformant onto the PDA plate to cultivate the transformant for 1-5 days at the temperature of 25-30 DEG C, and carrying out the subculture for 5-8 times. The method is easy and convenient to carry out, and high in conversion rate, transformant genetic stability is achived, protoplast is needless to be prepared, and complicated devices are needless.

The invention discloses mixed fermentation liquid inducing botrytis cinerea resistance and a preparation method of the mixed fermentation liquid. The mixed fermentation liquid is prepared by mixed fermentation of rhizopus nigricans with a preservation number of CGMCC NO. 8436 and trichoderma pseudokoningii with a preservation number of CGMCC NO. 1443. The mixed fermentation liquid can obviously improve induced resistance of tomatoes against botrytis; the rhizopus nigricans and the trichoderma pseudokoningii used in the mixed fermentation liquid are derived from a natural environment and can effectively induce the systematic resistance of the tomatoes against botrytis cinerea; a use method of the mixed fermentation liquid is simple and convenient; the mixed fermentation liquid has no environment pollution and is nontoxic to human and livestock; a production method of the fermentation liquid is simple and convenient and short in period; the used raw materials are cheap; large-scale massive production is easy; the production cost is low.

The invention discloses a preparation method of a biological fertilizer with a function of soil remediation. With nitrogen-fixing bacteria, bacillus mucilaginosus and rhizopus nigricans as compound bacteria, and iron element modified hydrotalcite as a template, the biological fertilizer can effectively immobilize a variety of heavy metal elements in soil and especially has extremely good immobilization effect on cadmium, chromium and mercury elements, so heavy metal elements in the soil can no longer enter plant bodies and water bodies, and the function of soil remediation can be effectively achieved.

The invention provides a preparation method of an organic fermented fertilizer. The preparation method comprises the following steps: (1) taking pitaya peel, watermelon peel and mango peel, dicing the peel, conducting pre-freezing, and then performing freeze-drying and crushing to obtain composite peel powder; (2) taking coconut residues and Chinese fevervine herb leaf residues, adding a fermentation inoculant I composed of bacillus licheniformis and aspergillus niger, and carrying out primary fermentation to obtain a mixed residue fermentation material; and (3) adding the composite peel powder into the mixed residue fermentation material, conducting mixing, adding a fermentation inoculant II composed of kloeckera apiculata, paenibacillus polymyxa and rhizopus nigricans, conducting stirring, and carrying out secondary fermentation to obtain the organic fermentation fertilizer. The fertilizer has comprehensive antiviral and growth-promoting effects on Capsicum chinense Jacquin and the like, improves crop yield and crop quality, and realizes efficient planting.

The invention provides a composite microbial agent with a biocontrol function and a preparation method and application of the composite microbial agent, and belongs to the technical field of bio-organic fertilizers. By optimizing the proportion, the multifunctional effect of the compound microbial agent is fully exerted, antagonism does not exist between the compound microbial agent and the compound microbial agent, the effective viable count reaches the maximum, and the compound microbial agent has the effects of generating active substances for promoting crop growth, supplying fertilizer tocrops and leading and planting beneficial microorganism dominant groups to crop root system soil, meanwhile, the trichoderma harzianum in the microbial agent is a biocontrol bacterium which is commonly used for preventing plant diseases caused by pathogenic bacteria such as pythium, rhizoctonia solani, fusarium, rhizopus nigricans, cylindrosporium, sclerotinia sclerotiorum, sclerotium rolfsii andthe like, and can effectively prevent soil-borne diseases of crops.

The invention relates to a multifunctional plant conditioning matrix. The multifunctional plant conditioning matrix comprises, by weight, 20 to 300 parts of a rooting agent, 10 to 70 parts of a water-retaining agent, 1000 to 2000 parts of soil, and 10 to 50 parts of a heavy metal ion adsorbent. The heavy metal ion adsorbent comprises orange peel, corn, and bran which are taken as base materials, and a mixed bacterial agent which is taken as a modifier and comprises beer yeast, Gordon amarae, aspergillus oryzae, cladosporium sp, and rhizopus nigricans. Beneficial effects are that: organic combination of agricultural crop with bacteria is realized; heavy metal ions can be absorbed effectively; water retention, slow release, fertilizer retention, soil improvement, and biological activation effects are achieved; transplanted plant survival rate is increased, and soil structure is improved at the same time; the ingredients of the multifunctional plant conditioning matrix are degradable organic matters, so that environmental pollution is not caused.

The invention discloses a preparation method of fungal symbiotically-fermented anti-tumor turmeric. The preparation method comprises the following steps: washing rice / glutinous rice, soaking, and drying through filtration; adding turmeric powder in proportion to obtain a mixture, wherein the addition ratio of the turmeric powder to the rice / glutinous rice is (1-30):(70-99); autoclaving or steamingthe mixture; cooling the autoclaved or steamed mixture; inoculating the cooled mixture with monascus and rhizopus nigricans; uniformly stirring the inoculated mixture, and placing in a container forfermentation; drying the fermented mixture; crushing the dried mixture; packaging the crushed mixture as required to obtain a finished product. Correspondingly, the invention further provides the fungal symbiotically-fermented anti-tumor turmeric prepared by the preparation method. According to the invention, the fungal symbiotically-fermented anti-tumor turmeric has an obvious anti-tumor effect,and side effects such as contact dermatitis, gastrointestinal pain, regurgitation, vomit and diarrhea of the turmeric are removed.

The invention relates to high-dietary-fiber fermented purple glutinous rice capable of replenishing blood. The high-dietary-fiber fermented purple glutinous rice is prepared from polished glutinous rice, purified water, bean dregs, red jujube juice, rhizopus nigricans, microzymes, acid protease and the like, and is prepared through the steps of cleaning, soaking, steaming, cooling, preparing, inoculating, fermenting, subpacking, sterilizing, cooling and the like. According to the high-dietary-fiber fermented purple glutinous rice capable of replenishing blood, a specific formula cooperates with a specific preparation method, so that the high-dietary-fiber fermented purple glutinous rice is thorough in fermentation, and is free from the phenomenon that the high-dietary-fiber fermented purple glutinous rice is too sour. The high-dietary-fiber fermented purple glutinous rice is rich in flavor, and good in mouth feel, and can be accepted by vast consumers, the content of the dietary fibersis as high as 1.50%, and when the high-dietary-fiber fermented purple glutinous rice and rice dumpling are eaten, the rice dumplings can be easier to digest. The high-dietary-fiber fermented purple glutinous rice has the function of replenishing blood, is particularly suitable for crowds of lying-in women, crows suffering from anemia and the like. Finished products are clear and transparent, freefrom phenomena of being precipitated and being turbid, and good in stability, and the shelf life is as long as 12 months. The embodiment of the high-dietary-fiber fermented purple glutinous rice is simple and easy to implement, and industrialized production is easy to realize.

The invention discloses a genetically engineered bacterium for high yield of ergothioneine, and belongs to the field of genetic engineering. The ergothioneine synthesis yield of the recombinant bacterium BWEGT-12 provided by the invention reaches the highest within 72 hours, and the yield is 110 mg / L. According to the method, ergothioneine synthesis gene clusters with different sources are screened firstly, ergothioneine synthesis genes from rhizopus nigricans and radiation-resistant red bacillus are screened, and the yield of 65mg / L and 77mg / L of ergothioneine is achieved preliminarily. Furthermore, chassis cells are improved, enhanced expression or knockout is carried out on related genes in metabolic pathways of histidine, cysteine and methionine, and the yield of 81mg / L of ergothioneine is achieved. In order to achieve higher yield, genes in the whole ways of methionine circulation and folic acid circulation are adjusted in a targeted manner, and finally the yield of 110mg / L of ergothioneine is achieved. According to the invention, efficient synthesis of ergothioneine is realized in escherichia coli, and the market supply of ergothioneine can be expanded.

The invention provides a method for preparing an efficient decomposition agent special for edible mushroom dregs. The main components of the decomposition agent are kluyveromyces marxianus, bacillus mucilaginosus and rhizopus nigricans, the specially prepared mixed bacterial species is good in decomposition effect, more than half of the time is saved compared with traditional methods, the cost is low, and the effect is good; the mushroom dreg decomposition material prepared by the decomposition agent is applied to the formula of a microbial fertilizer, other raw materials such as microelements are not required to be added besides the mushroom dreg decomposition material, vermiculite, traditional nitrogen phosphorus, potassium fertilizers and water, the cost is low, and anthrax disease of the sugar orange can be effectively prevented and treated.