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Rhizopus nigricans polysaccharide with antitumor activity and application thereof

A technology of Rhizopus niger and Rhizopus niger, applied in the field of biomedicine, can solve the problems of unsynthesizable natural polysaccharides, high development cost, resource limitation, etc., and achieve the effects of reducing tumor volume and weight, increasing body weight, and inhibiting cell growth.

Inactive Publication Date: 2014-09-03
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Natural polysaccharides are almost impossible to synthesize, and most of the polysaccharide drugs currently used clinically or under research are limited by resources and the development cost is high

Method used

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  • Rhizopus nigricans polysaccharide with antitumor activity and application thereof
  • Rhizopus nigricans polysaccharide with antitumor activity and application thereof
  • Rhizopus nigricans polysaccharide with antitumor activity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A strain of Rhizopus nigricans RN-9, which has the activity of producing rhizopus nigricans hyphal polysaccharides that inhibit the growth of gastric cancer BGC-823 cells, was preserved in the China Committee for Microorganism Culture Collection on November 5, 2013. Center, address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, deposit number CGMCC NO.8436.

[0050] Strain characteristics: creeping hyphae, well-developed roots, many branches, brown, the distance between two nodes is 1-3 mm; sporangia are spherical or oval, brown, 65-350 microns in diameter, with small crystals on the membrane, easy to dissolve; Cystic axis spherical, elliptical, ovoid or irregular, thin and smooth membrane, light brown, about 70 microns in diameter, about 90 microns in height; central axis base exists, 25-214 microns in diameter; spore shape is asymmetrical, subspherical, egg Shaped or polygonal, with lines on the s...

Embodiment 2

[0052] The application of Rhizopus nigricans (Rhizopus nigricans) RN-9 described in embodiment 1 in the preparation of Rhizopus nigricans mycelia polysaccharide with the activity of inhibiting the growth of gastric cancer BGC-823 cells, the steps are as follows:

[0053] (1) Inoculate Rhizopus nigricans RN-9 with strain preservation number CGMCC NO.8436 in PDA medium, and activate it at 28°C for 24 hours to obtain activated hyphae;

[0054] The PDA medium has the following components per liter: 200 g of potatoes, 20 g of sucrose, 2 g of agar, distilled tap water to 1000 mL, and natural pH;

[0055] (2) Transfer the activated mycelium prepared in step (1) to the PDB fermentation medium, culture at 28 ± 1° C., cultivate on a shaker at 120 rpm for 10 days, and filter with 4 layers of gauze to obtain liquid fermentation mycelia;

[0056] The potato sucrose liquid culture medium has the following components per liter: 200g of potatoes, 20g of sucrose, tap water constant to 1000mL, ...

Embodiment 3

[0067] Effect of RPS administration in vitro on the proliferation activity of gastric cancer BGC-823 cells

[0068] The full name of MTT is 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, and the Chinese chemical name is 3-(4,5-Dimethylthiazol-2)-2,5-diphenyl tetrazolium bromide. MTT method, also known as MTT colorimetric method, is a method for detecting cell survival and growth. The detection principle is: succinate dehydrogenase in the mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-purple crystalline formazan and deposit in the cells, while dead cells have no such function. Dimethyl sulfoxide (DMSO) can dissolve formazan in cells, and its light absorption value is measured at a wavelength of 570nm with an enzyme-linked immunosorbent detector, which can indirectly reflect the number of living cells. Within a certain cell number range, the amount of MTT crystal formation is proportional to the cell number. This method has been w...

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Abstract

The invention relates to rhizopus nigricans polysaccharide which has the activity of inhibiting growth of a cell BGC-823 and an application thereof. Rhizopus nigricans RN-9 with the rhizopus nigricans polysaccharide which has the activity of inhibiting growth of a cell BGC-823 of gastric cancer is preserved in China General Microbiological Culture Collection Center on 5th, November, 2013 with the preservation number of CGMCC NO.8436. The invention further provides the application of RN-9 in preparing rhizopus nigricans polysaccharide which has the activity of inhibiting growth of a cell BGC-823 of gastric cancer. The RN-9 provided by the invention has the function of generating rhizopus nigricans polysaccharide which has the activity of inhibiting growth of the cell BGC-823 of gastric cancer.

Description

technical field [0001] The invention relates to a Rhizopus niger mycelia polysaccharide with the activity of inhibiting the growth of BGC-823 cells and an application thereof, belonging to the technical field of biomedicine. Background technique [0002] With the continuous growth of the world's total population and the intensification of population aging, as well as the increase in high-risk behaviors of cancer year by year, the number of cancer patients worldwide has increased dramatically. According to statistics from the American Cancer Society (ACS), about 7.6 million cancer patients died worldwide in 2008, of which 740,000 died from gastric cancer, and about 230,000 died of gastric cancer in China every year. Improper diet and Helicobacter pylori infection are important causes of gastric cancer. In my country, Linqu County in Shandong Province, Zhuanghe County in Liaoning Province, and Changle County in Fujian Province are the most representative areas with high incid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P19/04C08B37/00A61K31/715A61P35/00C12R1/845
Inventor 陈靠山陈国创张鹏英禹文茜
Owner SHANDONG UNIV
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