Application of rhizopus nigricans exopolysaccharides in preparation of medicine for treating or preventing gastrointestinal tumors

A technology of digestive tract tumor and Rhizopus niger, which is applied in the fields of medicine and health food

Inactive Publication Date: 2014-09-17
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the functional research on the exopolysaccharide of Rhizopus niger has become a hot spot in the research of black mold, but the research on

Method used

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  • Application of rhizopus nigricans exopolysaccharides in preparation of medicine for treating or preventing gastrointestinal tumors
  • Application of rhizopus nigricans exopolysaccharides in preparation of medicine for treating or preventing gastrointestinal tumors
  • Application of rhizopus nigricans exopolysaccharides in preparation of medicine for treating or preventing gastrointestinal tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation of embodiment 1 Rhizopus niger exopolysaccharide

[0041] (1) Take Rhizopus niger and inoculate it in PDA medium, activate and cultivate it under the condition of 28 ℃ for 3 days to obtain the activated mycelia; Cultivate under the condition of 120rpm / min for 7 days to obtain a fermented liquid;

[0042] The composition of each liter of PDA medium is as follows:

[0043] Potato 200g, sucrose 20g, agar 15g, tap water 1000mL, pH natural.

[0044] The composition per liter of liquid medium is as follows:

[0045] Liquid PDB fermentation medium, the components per liter are as follows: 200g potatoes, 20g sucrose, 1000mL tap water, natural pH.

[0046] (2) Filter the fermented liquid with four layers of gauze, remove mycelia and spores, collect the filtered fermented liquid, centrifuge at 4000rpm / min for 15min, collect the supernatant, and place the collected supernatant in a rotary evaporator to concentrate , concentrated to one-third of the original volum...

Embodiment 2

[0048] Example 2 Inhibitory effect of Rhizopus niger exopolysaccharide combined with cyclophosphamide on growth of gastric cancer xenografts in nude mice

[0049] Nude mice were inoculated with gastric cancer cell lines to establish a human gastric cancer nude mouse xenograft tumor model, and gastric cancer nude mice were treated by intragastric administration, and the effects of Rhizopus niger exopolysaccharide combined with chemotherapy drugs on gastric cancer tumor-bearing mice were observed.

[0050] experimental method:

[0051] Gastric cancer MGC803 cells in the logarithmic phase were collected and diluted to 5×10 in PBS 7 individual / mL. Under sterile conditions, inoculate 0.2 mL of cell suspension in the left armpit of nude mice, observe for 3-5 days, and wait for the emergence of hard nodules the size of rice grains in the armpit as a criterion for successful modeling.

[0052] When the tumor rapidly grew to a diameter of 5 mm, they were randomly divided into 4 grou...

Embodiment 3

[0061] Example 3 Inhibitory effect of Rhizopus niger exopolysaccharide on human rectal cancer xenograft tumor growth in nude mice

[0062] The inhibitory effect of EPS on colon cancer solid tumors was studied by establishing a human colon cancer SE480 cell xenograft mouse model.

[0063] experimental method:

[0064] Human colon cancer cell SE480 xenografts were taken, ground to make a cell suspension, and inoculated in the left axilla of 24 nude mice. The inoculated mice were randomly divided into 4 groups, 6 in each group, respectively model group, polysaccharide low-dose group (25mg / kg, the Rhizopus niger extracellular crude polysaccharide that embodiment 1 made), polysaccharide middle-dose group (50mg / kg, the rhizopus niger extracellular crude polysaccharide that embodiment 1 makes), polysaccharide high-dose group (75mg / kg, the rhizopus niger extracellular crude polysaccharide that embodiment 1 makes), the model group gavages the same dosage every day Normal saline, ad...

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Abstract

The invention relates to an application of rhizopus nigricans exopolysaccharides in preparation of a medicine for treating or preventing gastrointestinal tumors. The method for preparing the rhizopus nigricans exopolysaccharides comprises the following steps: (1) activating and performing fermental cultivation on rhizopus nigricans with a strain collection number of CGMCC NO.8436, and preparing fermentation liquor; (2) separating and purifying the fermentation liquor, and preparing a crude extracting solution; and (3) discoloring the crude extracting solution by resin columns, oscillating to remove proteins through a Seveage reagent, performing column chromatography purification on Sephadex G-50 sephadex, and preparing the rhizopus nigricans exopolysaccharides. An animal tumor model is established by utilizing the rhizopus nigricans exopolysaccharides, the tumor activity of an entity anti-alimentary system of the rhizopus nigricans exopolysaccharides is explored, the animal tumor experiment anti-tumor result proves that the rhizopus nigricans exopolysaccharides can obviously inhibit growth of tumor blocks of stomach cancer tumor mice and colon cancer tumor mice, and when the rhizopus nigricans exopolysaccharides are combined with modern chemotherapy drug cyclophosphamide, the anti-tumor curative effect is obviously higher than the anti-tumor effect of a single cyclophosphamide drug.

Description

technical field [0001] The invention relates to the application of Rhizopus niger exopolysaccharide in the preparation of medicines for treating or preventing digestive tract tumors, and belongs to the technical field of medicine and health food. Background technique [0002] With the development of the society and the rapid improvement of the economy, people's life rhythm is accelerated, the ways are diversified, and the air pollution is getting worse, the population is aging and other factors, resulting in a sharp increase in the sub-health population, and the incidence of malignant tumors in our country And the death rate continues to increase, and now the cancer death rate has risen to the first place, and has become a social issue that people are increasingly concerned about. Digestive tract tumors are common malignant tumors worldwide, mainly including gastric cancer, esophageal cancer, colorectal cancer and liver cancer. With the improvement of people's living standa...

Claims

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Application Information

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IPC IPC(8): A61K31/715C08B37/00A61P35/00
Inventor 陈靠山侯东于志丹禹文茜陈国创石磊周南
Owner SHANDONG UNIV
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