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Mixed fermentation liquid inducing botrytis cinerea resistance and preparation method of mixed fermentation liquid

A technology of tomato gray mold and mixed fermentation is applied in the field of biological control of tomato gray mold, which can solve the problems of harsh conditions such as temperature and light, restricting the safe production of tomato, slow effect of biological pesticides, etc. Ease of mass production, effect of inducing systemic resistance

Active Publication Date: 2017-06-06
SHANDONG AGRI & ENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, due to the lack of disease-resistant varieties in production, and Botrytis cinerea has produced resistance to a variety of chemical fungicides, the control effect is greatly reduced, and the pesticide application mainly targets the fruit, resulting in excessive pesticide residues in the fruit. Safe Production of Tomatoes
Vegetable farmers mainly use chemical pesticides to prevent and control the disease in production, which can restore 15-30% of the total output. However, agricultural product pollution incidents occur one after another, and food safety issues have become a hot topic in people's livelihood
[0004] At present, there are also biopesticides sold and used in the market to prevent and control tomato botrytis, but the existing biopesticides are mainly used after the occurrence of tomato botrytis, and the biopesticides are slow to take effect, and are relatively harsh on the temperature and light conditions of the use environment , and also have a certain impact on tomato yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] In one embodiment of the present application, the preparation method of the mixed fermented liquid that induces and resists Botrytis cinerea is provided, and the steps are as follows:

[0032] (1) The Rhizopus niger with the preservation number CGMCC NO.8436 and the Trichoderma pseudoconii with the preservation number CGMCC NO.1443 are activated; the method for the activation treatment is to inoculate the Rhizopus niger and the Trichoderma pseudoconii On the PDA plate medium, culture at 24°C for 48 hours.

[0033] (2) Inoculate the activated Rhizopus niger and Trichoderma pseudoconii into the fermentation medium, cultivate for 5-7 days under the conditions of 22-30°C and 100-130r / min, filter, and incubate at 35-45°C Concentrate the filtrate for 5-10 times to obtain the mixed fermentation liquid for inducing and resisting tomato cinerea.

[0034] Since the currently known function of the Rhizopus niger with the preservation number CGMCC NO.1443 is to produce rhizopus ni...

Embodiment 1

[0040] Embodiment 1: the preparation of the mixed fermented liquid that induces and resists tomato cinerea

[0041] (1) Activation of the strain

[0042] The Rhizopus niger with the preservation number CGMCC NO.8436 and the Trichoderma pseudoconii with the preservation number CGMCC No.1443 were inoculated on the PDA plate medium, and cultured at a constant temperature of 24° C. for 48 hours.

[0043] PDA plate medium: 200g of potatoes (peeled, dig out the bud eyes), 20g of glucose, 20g of agar, 1000ml of water, cut the potatoes into small pieces about 0.5cm thick, put them in water, boil for 20min, filter with 4 layers of gauze, and take the filtrate (Add water to make up to 1000ml), after boiling, add glucose and agar in turn, after completely dissolving, divide into 250ml Erlenmeyer flasks, the filling volume is 100ml, sterilize under high pressure steam at 126°C and 0.15MPa for 30min, pour plate, spare.

[0044] (2) Preparation of mixed fermentation broth:

[0045] Use a...

Embodiment 2

[0047] Embodiment 2: the preparation of the mixed fermented liquid that induces and resists tomato cinerea

[0048] (1) Activation of the strain

[0049] The Rhizopus niger with the preservation number CGMCC NO.8436 and the Trichoderma pseudoconii with the preservation number CGMCC No.1443 were inoculated on the PDA plate medium, and cultured at a constant temperature of 24° C. for 48 hours.

[0050] The PDA plate medium is the same as in Example 1.

[0051] (2) Preparation of mixed fermentation broth:

[0052] Use a Φ6mm hole puncher to make fungus cakes on the edge of the PDA medium covered with colonies of Trichoderma pseudokonii and Rhizopus niger, and inoculate the cakes of Trichoderma pseudokonii and Rhizopus niger at a ratio of 3:1 with a sterile inoculation loop In the PDB medium, cultivated in a constant temperature oscillator at 22°C and 130r / min for 7 days, filtered through 4 layers of gauze, and concentrated the filtrate 5 times at 35°C to obtain the mixed fermen...

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Abstract

The invention discloses mixed fermentation liquid inducing botrytis cinerea resistance and a preparation method of the mixed fermentation liquid. The mixed fermentation liquid is prepared by mixed fermentation of rhizopus nigricans with a preservation number of CGMCC NO. 8436 and trichoderma pseudokoningii with a preservation number of CGMCC NO. 1443. The mixed fermentation liquid can obviously improve induced resistance of tomatoes against botrytis; the rhizopus nigricans and the trichoderma pseudokoningii used in the mixed fermentation liquid are derived from a natural environment and can effectively induce the systematic resistance of the tomatoes against botrytis cinerea; a use method of the mixed fermentation liquid is simple and convenient; the mixed fermentation liquid has no environment pollution and is nontoxic to human and livestock; a production method of the fermentation liquid is simple and convenient and short in period; the used raw materials are cheap; large-scale massive production is easy; the production cost is low.

Description

technical field [0001] The invention relates to the technical field of biological control of Botrytis cinerea, in particular to a mixed fermentation liquid for inducing and resisting Botrytis cinerea and a preparation method thereof. Background technique [0002] Plant-induced disease resistance, also known as systemic acquired resistance (SAR), refers to the fact that certain parts of plants can be induced to produce some protective substances after being treated with low-toxicity or attenuated pathogenic bacteria or pathogenic bacteria metabolites, so that the plants are protected from The original susceptibility state is transformed into partial or overall resistance to a certain or a certain type of pathogen infection. The SAR induced by the inducer has the following four characteristics and advantages: First, it is systemic, that is, the reaction can spread from the induction site to the whole plant, can occur at the induction site, and can also be transmitted to the un...

Claims

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Application Information

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IPC IPC(8): C12P39/00A01N63/04A01P3/00C12R1/845C12R1/885
CPCA01N63/30C12P39/00
Inventor 国淑梅牛贞福高瑞杰于安军张鹤
Owner SHANDONG AGRI & ENG UNIV
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