Compound bacterial agent for efficiently converting pig excrements and preparation method and application thereof

Abstract

The invention relates to a compound bacterial agent for efficiently converting pig excrements, and a preparation method thereof comprises the following steps of: (1) respectively carrying out activation and expanding culture on bacillus subtilis powder and Lactobacillus plantarum powder to obtain two bacterial suspensions, then inoculating the two bacterial suspensions to a bacterial fermentation medium in a volume ratio of 1:1 for compound fermentation co-culture, and subsequently, filtering fermentation liquor, adding a protective agent to the obtained filtrate and carrying out spray drying to prepare compound bacterial powder; (2) respectively carrying out activation and expanding culture on mucor racemosus powder, aspergillus niger powder, Aspergillus oryzae powder, Rhizopus nigricans powder and Candida utilis powder to obtain five bacterial suspensions, then inoculating the five bacterial suspensions to a fungus fermentation medium in a volume ratio of 1:1:1:1:1 for ventilation compound co-culture, and subsequently filtering fermentation liquor, adding a protective agent to the obtained filtrate and carrying out spray drying to prepare compound fungus powder; and (3) metering the compound bacterial powder and the compound fungus powder in a weight ratio of 1:1 and evenly mixing.

Description

technical field [0001] The invention relates to a composite microbial agent for transforming pig excrement and a preparation method thereof, and the application of the composite microbial agent in preparing a fermentation bed for transforming pig excrement. Background technique [0002] Pork is one of the most popular meats among Chinese people, accounting for more than 60% of domestic meat consumption. As the world's largest pork producer, its pork output reached 59.7 million tons in 2009, and pork products accounted for more than half of the world's total. Large-scale farming has developed rapidly, and retail farming has grown steadily. At the same time, the pollution caused by the pig excrement produced by pig breeding to the surrounding environment is becoming increasingly serious, and with the enhancement of people's awareness of environmental protection, this problem has become more prominent. [0003] The use of microorganisms to decompose and transform pig excrement...

AI Technical Summary

Problems solved by technology

Existing various composite microbial bacterial agents that are used to raise pigs in fermented beds, one class is formed by natural inoculation and natural multiplication (such as the invention patent application with publication number CN 101445312A), the inoculum amount and microbial strain composition are unstable, The number of miscellaneous bacteria is large, it is difficult to avoid the growth of harmful bacteria and pathogenic bacteria, and due to the small inoculation amount of natural inoculation, the growth is slow and the conversion efficiency is low; the other type is to make various single bacteria powders separately and mix them in proportion to make composite bacteria agents (For example, the publication number is the invention patent application of CN 101463335A), and its shortcoming is: after being connected to the fermentation bed, various microorganisms need to compete and interact with each other through a long period of time before they can form a relatively stable symbiotic system again. It needs a long adaptation period to start a large number of proliferation and fermentation, the lag period is long, and the conversion efficiency is not high