Food waste fermentation composite bacteria and preparation method thereof
A technology for fermenting compound bacteria and kitchen waste, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve the problems of polluted land, air and water sources, heavy odor of garbage dumps, human body hazards, etc. Prevention and control of soil-borne diseases, strong lipolytic function, and strong amylase activity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0043] High-density culture of Trichoderma viride, Bacillus subtilis, Rhizopus niger, photosynthetic bacteria, Saccharomyces cerevisiae and Bacillus licheniformis:
[0044] (1) Trichoderma viride: Inoculate the Trichoderma viride strain on the purified slant medium into a shake flask filled with an activated medium with a volume ratio of 15%, and the activated medium is wort potato sucrose medium , at a culture temperature of 30°C and a shaker flask rotation speed of 180rpm, activate and cultivate for 32 hours; inoculate the Trichoderma viride strains that have been activated and cultured into a fermenter with a volume ratio of 50% fermentation medium, and the inoculum volume is 1% by volume , the formula of the fermentation medium is glucose 8g / l, corn cob 5g / l, calcium chloride 0.2g / l, magnesium sulfate 0.1g / l, potassium dihydrogen phosphate 18g / l, yeast extract 8g / l, sulfuric acid Ferrous 3mg / l, zinc sulfate 1.0mg / l, cobalt chloride 3.5mg / l and manganese sulfate 1.2mg / l, at...
Embodiment 2
[0052] High-density culture of Trichoderma viride, Bacillus subtilis, Rhizopus niger, photosynthetic bacteria, Saccharomyces cerevisiae and Bacillus licheniformis:
[0053] (1) Trichoderma viride: Inoculate the Trichoderma viride strain on the purified slant culture medium into a shaker flask filled with a 30% volume ratio of activated medium, which is wort potato sucrose medium , at a culture temperature of 30°C and a shaker flask rotation speed of 140rpm, activate and cultivate for 48 hours; inoculate the Trichoderma viride strains that have been activated and cultured into a fermenter with a volume ratio of 70% fermentation medium, and the inoculum volume is 3% by volume , the formula of the fermentation medium is glucose 12g / l, corn cob 6g / l, calcium chloride 0.6g / l, magnesium sulfate 0.5g / l, potassium dihydrogen phosphate 22g / l, yeast extract 12g / l, sulfuric acid Ferrous 7mg / l, zinc sulfate 1.8mg / l, cobalt chloride 4.0mg / l and manganese sulfate 1.8mg / l, at a culture tempe...
Embodiment 3
[0061] High-density culture of Trichoderma viride, Bacillus subtilis, Rhizopus niger, photosynthetic bacteria, Saccharomyces cerevisiae and Bacillus licheniformis:
[0062] (1) Trichoderma viride: inoculate the Trichoderma viride strain on the purified slant medium into a shaker flask filled with an activated medium with a volume ratio of 20%, and the activated medium is wort potato sucrose medium , at a culture temperature of 30°C and a shaker flask rotation speed of 160rpm, activate and cultivate for 36 hours; inoculate the Trichoderma viride strains that have been activated and cultured into a fermenter with a volume ratio of 60% fermentation medium, and the inoculum volume is 2% by volume , the formula of the fermentation medium is glucose 10g / l, corn cob 5.5g / l, calcium chloride 0.4g / l, magnesium sulfate 0.3g / l, potassium dihydrogen phosphate 20g / l, yeast extract 10g / l, Ferrous sulfate 5mg / l, zinc sulfate 1.5mg / l, cobalt chloride 3.7mg / l and manganese sulfate 1.6mg / l, at ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com